The Influence of Collagen III Expression on the Efficiency of Cell Isolation With the Use of Collagenase H

Abstract Objective We previously demonstrated that collagenase H (ColH) plays a crucial role in rat islet isolation, whereas collagenase G (ColG) plays only a supporting role. We also showed that collagen III appears to be one of the key targets of ColH based on a mass spectrometry analysis. In the...

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Veröffentlicht in:	Transplantation proceedings 2014-07, Vol.46 (6), p.1942-1944
Hauptverfasser:	Yoshida, S, Yamagata, Y, Murayama, K, Watanabe, K, Imura, T, Igarashi, Y, Inagaki, A, Fujimori, K, Ohashi, K, Ohuchi, N, Satomi, S, Goto, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Separation Collagen - metabolism Collagenases - metabolism Hepatocytes - metabolism Islets of Langerhans - cytology Islets of Langerhans Transplantation Pancreas - metabolism Rats, Inbred Lew Surgery Thermolysin
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