Cryopreservation of Acropora digitifera sperm with use of sucrose and methanol based solution
Coral biodiversity has recently been considered an important topic in environmental studies. Biodiversity could be preserved with successful cryopreservation of endangered species gametes or embryos. Herein, we developed cryopreservation protocols for Acropora digitifera sperm with use of sucrose an...
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| Veröffentlicht in: | Cryobiology 2014-08, Vol.69 (1), p.134-139 |
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| container_title | Cryobiology |
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| creator | Ohki, Shun Morita, Masaya Kitanobo, Seiya Kowalska, Agata A. Kowalski, Radosław Kajetan |
| description | Coral biodiversity has recently been considered an important topic in environmental studies. Biodiversity could be preserved with successful cryopreservation of endangered species gametes or embryos. Herein, we developed cryopreservation protocols for Acropora digitifera sperm with use of sucrose and methanol based extender. We studied cryopreservation of A. digitifera sperm with floating frames, allowing the placement of 250μl French straws 4cm above the liquid nitrogen surface, resulting in a 40°C/min freezing rate. This method enabled the successful cryopreservation of sperm in 0.9M sucrose supplemented with 20% methanol. In this protocol, we used a 1:3 (sperm:extender) dilution ratio. The fertilization ratios of freezing:thawed sperm were similar to the control and reached 63%. This method might be a valuable option in the formation of A. digitifera gene banking. Further studies are needed to explore possibilities of using this method in cryopreservation of other coral’s sperm. |
| doi_str_mv | 10.1016/j.cryobiol.2014.06.005 |
| format | Article |
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Biodiversity could be preserved with successful cryopreservation of endangered species gametes or embryos. Herein, we developed cryopreservation protocols for Acropora digitifera sperm with use of sucrose and methanol based extender. We studied cryopreservation of A. digitifera sperm with floating frames, allowing the placement of 250μl French straws 4cm above the liquid nitrogen surface, resulting in a 40°C/min freezing rate. This method enabled the successful cryopreservation of sperm in 0.9M sucrose supplemented with 20% methanol. In this protocol, we used a 1:3 (sperm:extender) dilution ratio. The fertilization ratios of freezing:thawed sperm were similar to the control and reached 63%. This method might be a valuable option in the formation of A. digitifera gene banking. 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All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-79c42aeee3ba22f0cef89700054e2aea83d0bb5614577fdcce96c10bc585fd7d3</citedby><cites>FETCH-LOGICAL-c401t-79c42aeee3ba22f0cef89700054e2aea83d0bb5614577fdcce96c10bc585fd7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cryobiol.2014.06.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24974821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohki, Shun</creatorcontrib><creatorcontrib>Morita, Masaya</creatorcontrib><creatorcontrib>Kitanobo, Seiya</creatorcontrib><creatorcontrib>Kowalska, Agata A.</creatorcontrib><creatorcontrib>Kowalski, Radosław Kajetan</creatorcontrib><title>Cryopreservation of Acropora digitifera sperm with use of sucrose and methanol based solution</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>Coral biodiversity has recently been considered an important topic in environmental studies. Biodiversity could be preserved with successful cryopreservation of endangered species gametes or embryos. Herein, we developed cryopreservation protocols for Acropora digitifera sperm with use of sucrose and methanol based extender. We studied cryopreservation of A. digitifera sperm with floating frames, allowing the placement of 250μl French straws 4cm above the liquid nitrogen surface, resulting in a 40°C/min freezing rate. This method enabled the successful cryopreservation of sperm in 0.9M sucrose supplemented with 20% methanol. In this protocol, we used a 1:3 (sperm:extender) dilution ratio. The fertilization ratios of freezing:thawed sperm were similar to the control and reached 63%. This method might be a valuable option in the formation of A. digitifera gene banking. Further studies are needed to explore possibilities of using this method in cryopreservation of other coral’s sperm.</description><subject>Animals</subject><subject>Anthozoa</subject><subject>Biodiversity</subject><subject>Coral</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Endangered Species</subject><subject>Fertilization in Vitro</subject><subject>Male</subject><subject>Methanol - pharmacology</subject><subject>Semen Analysis</subject><subject>Semen Preservation - methods</subject><subject>Sperm</subject><subject>Sperm Motility</subject><subject>Sucrose - pharmacology</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9LxDAQxYMouq5-BenRS-skTdPtTVn8B4IXPUpIk6mbpW1q0ir77U1Z9epphuE3b948Qi4oZBSouNpm2u9cbV2bMaA8A5EBFAdkQaGClOUVOyQLAEpTxjickNMQtgAgypwfkxPGq5KvGF2Qt3WUGTwG9J9qtK5PXJPcaO8G51Vi7LsdbYOxDQP6Lvmy4yaZAs5UmCIWW9WbpMNxo3rXJrUKaJLg2mkWOyNHjWoDnv_UJXm9u31ZP6RPz_eP65unVHOgY1pWmjOFiHmtGGtAY7Oqymi34BjnapUbqOtCUF6UZWO0xkpoCrUuVkVjSpMvyeVed_DuY8Iwys4GjW2renRTkLQo8hKEABpRsUdn88FjIwdvO-V3koKco5Vb-RutnKOVIGR0Ehcvfm5MdYfmb-03ywhc7wGMn35a9DJoi71GYz3qURpn_7vxDfUQkC4</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Ohki, Shun</creator><creator>Morita, Masaya</creator><creator>Kitanobo, Seiya</creator><creator>Kowalska, Agata A.</creator><creator>Kowalski, Radosław Kajetan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140801</creationdate><title>Cryopreservation of Acropora digitifera sperm with use of sucrose and methanol based solution</title><author>Ohki, Shun ; Morita, Masaya ; Kitanobo, Seiya ; Kowalska, Agata A. ; Kowalski, Radosław Kajetan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-79c42aeee3ba22f0cef89700054e2aea83d0bb5614577fdcce96c10bc585fd7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Anthozoa</topic><topic>Biodiversity</topic><topic>Coral</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Endangered Species</topic><topic>Fertilization in Vitro</topic><topic>Male</topic><topic>Methanol - pharmacology</topic><topic>Semen Analysis</topic><topic>Semen Preservation - methods</topic><topic>Sperm</topic><topic>Sperm Motility</topic><topic>Sucrose - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohki, Shun</creatorcontrib><creatorcontrib>Morita, Masaya</creatorcontrib><creatorcontrib>Kitanobo, Seiya</creatorcontrib><creatorcontrib>Kowalska, Agata A.</creatorcontrib><creatorcontrib>Kowalski, Radosław Kajetan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohki, Shun</au><au>Morita, Masaya</au><au>Kitanobo, Seiya</au><au>Kowalska, Agata A.</au><au>Kowalski, Radosław Kajetan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of Acropora digitifera sperm with use of sucrose and methanol based solution</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>69</volume><issue>1</issue><spage>134</spage><epage>139</epage><pages>134-139</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>Coral biodiversity has recently been considered an important topic in environmental studies. Biodiversity could be preserved with successful cryopreservation of endangered species gametes or embryos. Herein, we developed cryopreservation protocols for Acropora digitifera sperm with use of sucrose and methanol based extender. We studied cryopreservation of A. digitifera sperm with floating frames, allowing the placement of 250μl French straws 4cm above the liquid nitrogen surface, resulting in a 40°C/min freezing rate. This method enabled the successful cryopreservation of sperm in 0.9M sucrose supplemented with 20% methanol. In this protocol, we used a 1:3 (sperm:extender) dilution ratio. The fertilization ratios of freezing:thawed sperm were similar to the control and reached 63%. This method might be a valuable option in the formation of A. digitifera gene banking. 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| ispartof | Cryobiology, 2014-08, Vol.69 (1), p.134-139 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Anthozoa Biodiversity Coral Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Endangered Species Fertilization in Vitro Male Methanol - pharmacology Semen Analysis Semen Preservation - methods Sperm Sperm Motility Sucrose - pharmacology |
| title | Cryopreservation of Acropora digitifera sperm with use of sucrose and methanol based solution |
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