Outbreaks and genetic diversity of Francisella noatunensis subsp orientalis isolated from farm-raised Nile tilapia (Oreochromis niloticus) in Brazil
Francisella noatunensis subsp orientalis (FNO) is an emerging pathogen of warm water tilapia in a number of different countries. The disease caused by this bacterium in fish is characterized by a systemic granulomatous infection that causes high mortality rates during outbreaks. FNO has been previou...
Gespeichert in:
| Veröffentlicht in: | Genetics and molecular research 2014-07, Vol.13 (3), p.5704-5712 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5712 |
|---|---|
| container_issue | 3 |
| container_start_page | 5704 |
| container_title | Genetics and molecular research |
| container_volume | 13 |
| creator | Leal, C A G Tavares, G C Figueiredo, H C P |
| description | Francisella noatunensis subsp orientalis (FNO) is an emerging pathogen of warm water tilapia in a number of different countries. The disease caused by this bacterium in fish is characterized by a systemic granulomatous infection that causes high mortality rates during outbreaks. FNO has been previously described in Asia, Europe, and Central and North America. Its occurrence in South America has never been described. Since 2012, outbreaks of a granulomatous disease have been recorded in cage farms of Nile tilapia (Oreochromis niloticus L.) in Brazil. The current study aimed to identify the etiologic agent of recent francisellosis outbreaks at Brazilian tilapia farms, and to characterize the genetic diversity of the pathogen from farms with distinct geographic origins and without epidemiological connections. Bacteriological analysis of 44 diseased Nile tilapia collected from five cage farms in Brazil was performed during 2012 and 2013. The farms were in different locations and had no recent history of animal or biological material transport between each other. Sixty-two FNO isolates were identified on the basis of FNO-specific qPCR. The main predisposing factors for the occurrence of outbreaks on Brazilian farms were lower water temperature ( |
| doi_str_mv | 10.4238/2014.July.25.26 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1553321445</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1553321445</sourcerecordid><originalsourceid>FETCH-LOGICAL-c338t-fea864ccae1c8835e4f76cc7d94ea17a7471b3da0b15398e0facf72149eee6d13</originalsourceid><addsrcrecordid>eNpNkctuFDEQRS0EIiGwZoe8DIue-NntWUJEeChiNrC2qt1lMHjswXYjDd_BB-NRAmJVpdK59bqEPOdso4Q0V4JxtfmwxuNG6I0YH5BzPk7joEfDHv6Xn5EntX5jTGhl2GNyJjTnkxTmnPzerW0uCN8rhbTQL5iwBUeX8BNLDe1Is6c3BZILFWMEmjK0NWGqodK6zvVAcwmYGsReCDVHaLhQX_Keeij7oUAXLvRjiEhbiHAIQC93BbP72pmuSSHmPnGtL2lI9HWBXyE-JY88xIrP7uMF-Xzz5tP1u-F29_b99avbwUlp2uARzKicA-TOGKlR-Wl0blq2CoFPMKmJz3IBNnMttwaZB-cnwdUWEceFywtyedf3UPKPFWuzfSN3ujNhXqvlWkvZeaU7enWHupJrLejtoYQ9lKPlzJ6ssCcr7MkKK7QVY1e8uG--zntc_vF_fy__AFlRiaY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1553321445</pqid></control><display><type>article</type><title>Outbreaks and genetic diversity of Francisella noatunensis subsp orientalis isolated from farm-raised Nile tilapia (Oreochromis niloticus) in Brazil</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Leal, C A G ; Tavares, G C ; Figueiredo, H C P</creator><creatorcontrib>Leal, C A G ; Tavares, G C ; Figueiredo, H C P</creatorcontrib><description>Francisella noatunensis subsp orientalis (FNO) is an emerging pathogen of warm water tilapia in a number of different countries. The disease caused by this bacterium in fish is characterized by a systemic granulomatous infection that causes high mortality rates during outbreaks. FNO has been previously described in Asia, Europe, and Central and North America. Its occurrence in South America has never been described. Since 2012, outbreaks of a granulomatous disease have been recorded in cage farms of Nile tilapia (Oreochromis niloticus L.) in Brazil. The current study aimed to identify the etiologic agent of recent francisellosis outbreaks at Brazilian tilapia farms, and to characterize the genetic diversity of the pathogen from farms with distinct geographic origins and without epidemiological connections. Bacteriological analysis of 44 diseased Nile tilapia collected from five cage farms in Brazil was performed during 2012 and 2013. The farms were in different locations and had no recent history of animal or biological material transport between each other. Sixty-two FNO isolates were identified on the basis of FNO-specific qPCR. The main predisposing factors for the occurrence of outbreaks on Brazilian farms were lower water temperature (<22°C) and life stage of fish, affecting mainly fry, fingerlings and young adults (live weight <100 g). The genetic diversity of the Brazilian FNO isolates was evaluated using repetitive extragenic palindromic-PCR. The isolates from different origins were shown to be clonally related. This is the first report of the occurrence and genetic diversity of FNO in South America.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/2014.July.25.26</identifier><identifier>PMID: 25117328</identifier><language>eng</language><publisher>Brazil</publisher><subject>Animals ; Animals, Domestic ; Brazil - epidemiology ; Cichlids - microbiology ; Cluster Analysis ; Disease Outbreaks ; DNA, Bacterial ; Fish Diseases - epidemiology ; Fish Diseases - microbiology ; Francisella - classification ; Francisella - genetics ; Francisella - isolation & purification ; Genetic Variation ; Gram-Negative Bacterial Infections - veterinary ; Ribotyping ; RNA, Ribosomal, 16S - genetics</subject><ispartof>Genetics and molecular research, 2014-07, Vol.13 (3), p.5704-5712</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-fea864ccae1c8835e4f76cc7d94ea17a7471b3da0b15398e0facf72149eee6d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25117328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leal, C A G</creatorcontrib><creatorcontrib>Tavares, G C</creatorcontrib><creatorcontrib>Figueiredo, H C P</creatorcontrib><title>Outbreaks and genetic diversity of Francisella noatunensis subsp orientalis isolated from farm-raised Nile tilapia (Oreochromis niloticus) in Brazil</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Francisella noatunensis subsp orientalis (FNO) is an emerging pathogen of warm water tilapia in a number of different countries. The disease caused by this bacterium in fish is characterized by a systemic granulomatous infection that causes high mortality rates during outbreaks. FNO has been previously described in Asia, Europe, and Central and North America. Its occurrence in South America has never been described. Since 2012, outbreaks of a granulomatous disease have been recorded in cage farms of Nile tilapia (Oreochromis niloticus L.) in Brazil. The current study aimed to identify the etiologic agent of recent francisellosis outbreaks at Brazilian tilapia farms, and to characterize the genetic diversity of the pathogen from farms with distinct geographic origins and without epidemiological connections. Bacteriological analysis of 44 diseased Nile tilapia collected from five cage farms in Brazil was performed during 2012 and 2013. The farms were in different locations and had no recent history of animal or biological material transport between each other. Sixty-two FNO isolates were identified on the basis of FNO-specific qPCR. The main predisposing factors for the occurrence of outbreaks on Brazilian farms were lower water temperature (<22°C) and life stage of fish, affecting mainly fry, fingerlings and young adults (live weight <100 g). The genetic diversity of the Brazilian FNO isolates was evaluated using repetitive extragenic palindromic-PCR. The isolates from different origins were shown to be clonally related. This is the first report of the occurrence and genetic diversity of FNO in South America.</description><subject>Animals</subject><subject>Animals, Domestic</subject><subject>Brazil - epidemiology</subject><subject>Cichlids - microbiology</subject><subject>Cluster Analysis</subject><subject>Disease Outbreaks</subject><subject>DNA, Bacterial</subject><subject>Fish Diseases - epidemiology</subject><subject>Fish Diseases - microbiology</subject><subject>Francisella - classification</subject><subject>Francisella - genetics</subject><subject>Francisella - isolation & purification</subject><subject>Genetic Variation</subject><subject>Gram-Negative Bacterial Infections - veterinary</subject><subject>Ribotyping</subject><subject>RNA, Ribosomal, 16S - genetics</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkctuFDEQRS0EIiGwZoe8DIue-NntWUJEeChiNrC2qt1lMHjswXYjDd_BB-NRAmJVpdK59bqEPOdso4Q0V4JxtfmwxuNG6I0YH5BzPk7joEfDHv6Xn5EntX5jTGhl2GNyJjTnkxTmnPzerW0uCN8rhbTQL5iwBUeX8BNLDe1Is6c3BZILFWMEmjK0NWGqodK6zvVAcwmYGsReCDVHaLhQX_Keeij7oUAXLvRjiEhbiHAIQC93BbP72pmuSSHmPnGtL2lI9HWBXyE-JY88xIrP7uMF-Xzz5tP1u-F29_b99avbwUlp2uARzKicA-TOGKlR-Wl0blq2CoFPMKmJz3IBNnMttwaZB-cnwdUWEceFywtyedf3UPKPFWuzfSN3ujNhXqvlWkvZeaU7enWHupJrLejtoYQ9lKPlzJ6ssCcr7MkKK7QVY1e8uG--zntc_vF_fy__AFlRiaY</recordid><startdate>20140725</startdate><enddate>20140725</enddate><creator>Leal, C A G</creator><creator>Tavares, G C</creator><creator>Figueiredo, H C P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140725</creationdate><title>Outbreaks and genetic diversity of Francisella noatunensis subsp orientalis isolated from farm-raised Nile tilapia (Oreochromis niloticus) in Brazil</title><author>Leal, C A G ; Tavares, G C ; Figueiredo, H C P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-fea864ccae1c8835e4f76cc7d94ea17a7471b3da0b15398e0facf72149eee6d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Animals, Domestic</topic><topic>Brazil - epidemiology</topic><topic>Cichlids - microbiology</topic><topic>Cluster Analysis</topic><topic>Disease Outbreaks</topic><topic>DNA, Bacterial</topic><topic>Fish Diseases - epidemiology</topic><topic>Fish Diseases - microbiology</topic><topic>Francisella - classification</topic><topic>Francisella - genetics</topic><topic>Francisella - isolation & purification</topic><topic>Genetic Variation</topic><topic>Gram-Negative Bacterial Infections - veterinary</topic><topic>Ribotyping</topic><topic>RNA, Ribosomal, 16S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leal, C A G</creatorcontrib><creatorcontrib>Tavares, G C</creatorcontrib><creatorcontrib>Figueiredo, H C P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leal, C A G</au><au>Tavares, G C</au><au>Figueiredo, H C P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Outbreaks and genetic diversity of Francisella noatunensis subsp orientalis isolated from farm-raised Nile tilapia (Oreochromis niloticus) in Brazil</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2014-07-25</date><risdate>2014</risdate><volume>13</volume><issue>3</issue><spage>5704</spage><epage>5712</epage><pages>5704-5712</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>Francisella noatunensis subsp orientalis (FNO) is an emerging pathogen of warm water tilapia in a number of different countries. The disease caused by this bacterium in fish is characterized by a systemic granulomatous infection that causes high mortality rates during outbreaks. FNO has been previously described in Asia, Europe, and Central and North America. Its occurrence in South America has never been described. Since 2012, outbreaks of a granulomatous disease have been recorded in cage farms of Nile tilapia (Oreochromis niloticus L.) in Brazil. The current study aimed to identify the etiologic agent of recent francisellosis outbreaks at Brazilian tilapia farms, and to characterize the genetic diversity of the pathogen from farms with distinct geographic origins and without epidemiological connections. Bacteriological analysis of 44 diseased Nile tilapia collected from five cage farms in Brazil was performed during 2012 and 2013. The farms were in different locations and had no recent history of animal or biological material transport between each other. Sixty-two FNO isolates were identified on the basis of FNO-specific qPCR. The main predisposing factors for the occurrence of outbreaks on Brazilian farms were lower water temperature (<22°C) and life stage of fish, affecting mainly fry, fingerlings and young adults (live weight <100 g). The genetic diversity of the Brazilian FNO isolates was evaluated using repetitive extragenic palindromic-PCR. The isolates from different origins were shown to be clonally related. This is the first report of the occurrence and genetic diversity of FNO in South America.</abstract><cop>Brazil</cop><pmid>25117328</pmid><doi>10.4238/2014.July.25.26</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1676-5680 |
| ispartof | Genetics and molecular research, 2014-07, Vol.13 (3), p.5704-5712 |
| issn | 1676-5680 1676-5680 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1553321445 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Animals, Domestic Brazil - epidemiology Cichlids - microbiology Cluster Analysis Disease Outbreaks DNA, Bacterial Fish Diseases - epidemiology Fish Diseases - microbiology Francisella - classification Francisella - genetics Francisella - isolation & purification Genetic Variation Gram-Negative Bacterial Infections - veterinary Ribotyping RNA, Ribosomal, 16S - genetics |
| title | Outbreaks and genetic diversity of Francisella noatunensis subsp orientalis isolated from farm-raised Nile tilapia (Oreochromis niloticus) in Brazil |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T03%3A56%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Outbreaks%20and%20genetic%20diversity%20of%20Francisella%20noatunensis%20subsp%20orientalis%20isolated%20from%20farm-raised%20Nile%20tilapia%20(Oreochromis%20niloticus)%20in%20Brazil&rft.jtitle=Genetics%20and%20molecular%20research&rft.au=Leal,%20C%20A%20G&rft.date=2014-07-25&rft.volume=13&rft.issue=3&rft.spage=5704&rft.epage=5712&rft.pages=5704-5712&rft.issn=1676-5680&rft.eissn=1676-5680&rft_id=info:doi/10.4238/2014.July.25.26&rft_dat=%3Cproquest_cross%3E1553321445%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1553321445&rft_id=info:pmid/25117328&rfr_iscdi=true |