False‐Positive Results in Metagenomic Virus Discovery: A Strong Case for Follow‐Up Diagnosis

A viral metagenomic approach using virion enrichment, random amplification and next‐generation sequencing was used to investigate an undiagnosed cluster of dairy cattle presenting with high persistent fever, unresponsive to anti‐microbial and anti‐inflammatory treatment, diarrhoea and redness of nos...

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Veröffentlicht in:	Transboundary and emerging diseases 2014-08, Vol.61 (4), p.293-299
Hauptverfasser:	Rosseel, T, Pardon, B, De Clercq, K, Ozhelvaci, O, Van Borm, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal diseases Animals bioinformatics blood serum Cattle Cattle Diseases - diagnosis Cattle Diseases - virology contamination dairy cattle diagnostic techniques Diagnostic tests diarrhea DNA viruses DNA, Viral - classification DNA, Viral - genetics False Positive Reactions Female fever Genomics Metagenomics next-generation sequencing nose nucleic acids Parvoviridae Infections - diagnosis Parvoviridae Infections - veterinary Parvoviridae Infections - virology parvovirus Real-Time Polymerase Chain Reaction - methods reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - genetics RNA, Viral - isolation & purification Specimen Handling - methods Veterinary medicine viral metagenomics virion viruses Viruses - genetics Viruses - isolation & purification
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