Liquid chromatographic determination of caffeine in biologic fluids
A high-performance liquid chromatographic procedure was developed for the determination of caffeine in various biologic fluids and coffee. A reversed-phase column and UV detection at 254 nm were used to obtain a sensitivity of 0.1 μg/ml caffeine in serum and saliva using a sample volume of 0.1 ml. C...
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| Veröffentlicht in: | Journal of Chromatography A 1982-01, Vol.229 (2), p.387-395 |
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| container_end_page | 395 |
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| container_issue | 2 |
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| container_title | Journal of Chromatography A |
| container_volume | 229 |
| creator | Haughey, David B. Greenberg, Richard Schaal, Stephen F. Lima, John J. |
| description | A high-performance liquid chromatographic procedure was developed for the determination of caffeine in various biologic fluids and coffee. A reversed-phase column and UV detection at 254 nm were used to obtain a sensitivity of 0.1 μg/ml caffeine in serum and saliva using a sample volume of 0.1 ml. Caffeine metabolites and commonly ingested xanthines do not interfere with the assay. The within-day coefficients of variation were 9.8 and 9.9% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. The day-to-day coefficients of variation were 6.8 and 6.6% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. Serum and saliva caffeine concentrations were determined following a single oral dose of coffee and an intravenous infusion of caffeine in one subject. Computer estimates of caffeine pharmacokinetic parameters in one subject are in excellent agreement with previously published values. |
| doi_str_mv | 10.1016/S0378-4347(00)84281-4 |
| format | Article |
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A reversed-phase column and UV detection at 254 nm were used to obtain a sensitivity of 0.1 μg/ml caffeine in serum and saliva using a sample volume of 0.1 ml. Caffeine metabolites and commonly ingested xanthines do not interfere with the assay. The within-day coefficients of variation were 9.8 and 9.9% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. The day-to-day coefficients of variation were 6.8 and 6.6% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. Serum and saliva caffeine concentrations were determined following a single oral dose of coffee and an intravenous infusion of caffeine in one subject. 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A reversed-phase column and UV detection at 254 nm were used to obtain a sensitivity of 0.1 μg/ml caffeine in serum and saliva using a sample volume of 0.1 ml. Caffeine metabolites and commonly ingested xanthines do not interfere with the assay. The within-day coefficients of variation were 9.8 and 9.9% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. The day-to-day coefficients of variation were 6.8 and 6.6% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. Serum and saliva caffeine concentrations were determined following a single oral dose of coffee and an intravenous infusion of caffeine in one subject. Computer estimates of caffeine pharmacokinetic parameters in one subject are in excellent agreement with previously published values.</description><subject>Caffeine - blood</subject><subject>Caffeine - pharmacology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Coffee - analysis</subject><subject>Humans</subject><issn>0378-4347</issn><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAUhT2ASin8hEqZEAwBPxNnQqjiJVViAGbLsa9boyRu7QSJf09Kq65MV7rnnPv4EJoTfEswKe7eMStlzhkvrzG-kZxKkvMTND22z9B5Sl8YM44pnaBJiauCl2yKFku_HbzNzDqGVvdhFfVm7U1moYfY-k73PnRZcJnRzoHvIPNdVvvQhNXocs2YTRfo1OkmweWhztDn0-PH4iVfvj2_Lh6WuWEF7nNBJK2MdLSgpKRVLQ1x0loidGkomJprArWGajyfE1fYsi6YMJLUhZBVLSyboav93E0M2wFSr1qfDDSN7iAMSREhGBGSj0axN5oYUorg1Cb6VscfRbDaAVN_wNSOjMJY_QFTu9z8sGCoW7DH1IHWqN_vdRi__PYQVTIeOgPWRzC9ssH_s-EXY2t82g</recordid><startdate>19820101</startdate><enddate>19820101</enddate><creator>Haughey, David B.</creator><creator>Greenberg, Richard</creator><creator>Schaal, Stephen F.</creator><creator>Lima, John J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19820101</creationdate><title>Liquid chromatographic determination of caffeine in biologic fluids</title><author>Haughey, David B. ; Greenberg, Richard ; Schaal, Stephen F. ; Lima, John J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-51829c8f2621729b8c1f8dd15a7c2ecb4a1ebae984241f6d7b635c81b6589b5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Caffeine - blood</topic><topic>Caffeine - pharmacology</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Coffee - analysis</topic><topic>Humans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haughey, David B.</creatorcontrib><creatorcontrib>Greenberg, Richard</creatorcontrib><creatorcontrib>Schaal, Stephen F.</creatorcontrib><creatorcontrib>Lima, John J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haughey, David B.</au><au>Greenberg, Richard</au><au>Schaal, Stephen F.</au><au>Lima, John J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid chromatographic determination of caffeine in biologic fluids</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr</addtitle><date>1982-01-01</date><risdate>1982</risdate><volume>229</volume><issue>2</issue><spage>387</spage><epage>395</epage><pages>387-395</pages><issn>0378-4347</issn><issn>0021-9673</issn><abstract>A high-performance liquid chromatographic procedure was developed for the determination of caffeine in various biologic fluids and coffee. A reversed-phase column and UV detection at 254 nm were used to obtain a sensitivity of 0.1 μg/ml caffeine in serum and saliva using a sample volume of 0.1 ml. Caffeine metabolites and commonly ingested xanthines do not interfere with the assay. The within-day coefficients of variation were 9.8 and 9.9% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. The day-to-day coefficients of variation were 6.8 and 6.6% at plasma caffeine concentrations of 2 and 10 μg/ml, respectively. Serum and saliva caffeine concentrations were determined following a single oral dose of coffee and an intravenous infusion of caffeine in one subject. Computer estimates of caffeine pharmacokinetic parameters in one subject are in excellent agreement with previously published values.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7096473</pmid><doi>10.1016/S0378-4347(00)84281-4</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0378-4347 |
| ispartof | Journal of Chromatography A, 1982-01, Vol.229 (2), p.387-395 |
| issn | 0378-4347 0021-9673 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | Caffeine - blood Caffeine - pharmacology Chromatography, High Pressure Liquid Coffee - analysis Humans |
| title | Liquid chromatographic determination of caffeine in biologic fluids |
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