The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine
This study investigated the effects of dietary valine on the growth, intestinal immune response, tight junction proteins transcript abundance and gene expression of immune-related signaling molecules in the intestine of young grass carp (Ctenopharyngodon idella). Six iso-nitrogenous diets containing...
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| Veröffentlicht in: | Fish & shellfish immunology 2014-09, Vol.40 (1), p.197-207 |
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| creator | Luo, Jian-Bo Feng, Lin Jiang, Wei-Dan Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Zhang, Yong-An Zhou, Xiao-Qiu |
| description | This study investigated the effects of dietary valine on the growth, intestinal immune response, tight junction proteins transcript abundance and gene expression of immune-related signaling molecules in the intestine of young grass carp (Ctenopharyngodon idella). Six iso-nitrogenous diets containing graded levels of valine (4.3–19.1 g kg−1 diet) were fed to the fish for 8 weeks. The results showed that percentage weight gain (PWG), feed intake and feed efficiency of fish were the lowest in fish fed the valine-deficient diet (P |
| doi_str_mv | 10.1016/j.fsi.2014.07.003 |
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•Valine deficiency decreased immune status of intestine.•Valine regulated the gene expression of NF-κB P65, IκBα, TOR.•Valine regulated tight junction proteins transcript abundance.]]></description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2014.07.003</identifier><identifier>PMID: 25014314</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animal Feed - analysis ; Animals ; Carps - genetics ; Carps - immunology ; Diet - veterinary ; Dietary Supplements - analysis ; Fish Proteins - genetics ; Fish Proteins - metabolism ; Gene Expression Regulation ; Grass carp ; I-kappa B Proteins - genetics ; I-kappa B Proteins - metabolism ; Immune status ; Immunity, Mucosal - drug effects ; Intestinal Mucosa - drug effects ; Intestine ; NF-KappaB Inhibitor alpha ; Tight junction proteins ; Tight Junction Proteins - genetics ; Tight Junction Proteins - metabolism ; TOR Serine-Threonine Kinases - genetics ; TOR Serine-Threonine Kinases - metabolism ; Transcription Factor RelA - genetics ; Transcription Factor RelA - metabolism ; Valine - pharmacology ; Valine deficiency</subject><ispartof>Fish & shellfish immunology, 2014-09, Vol.40 (1), p.197-207</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-fcfab86d3feb80a3d40e3eaf30e61d3ca3339e3e8c8ac64c607320ba17a524443</citedby><cites>FETCH-LOGICAL-c353t-fcfab86d3feb80a3d40e3eaf30e61d3ca3339e3e8c8ac64c607320ba17a524443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464814002447$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25014314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Jian-Bo</creatorcontrib><creatorcontrib>Feng, Lin</creatorcontrib><creatorcontrib>Jiang, Wei-Dan</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Wu, Pei</creatorcontrib><creatorcontrib>Jiang, Jun</creatorcontrib><creatorcontrib>Kuang, Sheng-Yao</creatorcontrib><creatorcontrib>Tang, Ling</creatorcontrib><creatorcontrib>Zhang, Yong-An</creatorcontrib><creatorcontrib>Zhou, Xiao-Qiu</creatorcontrib><title>The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description><![CDATA[This study investigated the effects of dietary valine on the growth, intestinal immune response, tight junction proteins transcript abundance and gene expression of immune-related signaling molecules in the intestine of young grass carp (Ctenopharyngodon idella). Six iso-nitrogenous diets containing graded levels of valine (4.3–19.1 g kg−1 diet) were fed to the fish for 8 weeks. The results showed that percentage weight gain (PWG), feed intake and feed efficiency of fish were the lowest in fish fed the valine-deficient diet (P < 0.05). In addition, valine deficiency decreased lysozyme, acid phosphatase activities and complement 3 content in the intestine (P < 0.05), down-regulated mRNA levels of interleukin 10, transforming growth factor β1, IκBα and target of rapamycin (TOR) (P < 0.05), and up-regulated tumor necrosis factor α, interleukin 8 and nuclear factor κB P65 (NF-κB P65) gene expression (P < 0.05). Additionally, valine deficiency significantly decreased transcript of Occludin, Claudin b, Claudin c, Claudin 3, and ZO-1 (P < 0.05), and improved Claudin 15 expression in the fish intestine (P < 0.05). However, valine did not have a significant effect on expression of Claudin 12 in the intestine of grass carp (P > 0.05). In conclusion, valine deficiency decreased fish growth and intestinal immune status, as well as regulated gene expression of tight junction proteins, NF-κB P65, IκBα and TOR in the fish intestine. Based on the quadratic regression analysis of lysozyme activity or PWG, the dietary valine requirement of young grass carp (268–679 g) were established to be 14.47 g kg−1 diet (4.82 g 100 g−1 CP) or 14.00 g kg−1 diet (4.77 g 100 g−1 CP), respectively.
•Valine deficiency decreased immune status of intestine.•Valine regulated the gene expression of NF-κB P65, IκBα, TOR.•Valine regulated tight junction proteins transcript abundance.]]></description><subject>Animal Feed - analysis</subject><subject>Animals</subject><subject>Carps - genetics</subject><subject>Carps - immunology</subject><subject>Diet - veterinary</subject><subject>Dietary Supplements - analysis</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Grass carp</subject><subject>I-kappa B Proteins - genetics</subject><subject>I-kappa B Proteins - metabolism</subject><subject>Immune status</subject><subject>Immunity, Mucosal - drug effects</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestine</subject><subject>NF-KappaB Inhibitor alpha</subject><subject>Tight junction proteins</subject><subject>Tight Junction Proteins - genetics</subject><subject>Tight Junction Proteins - metabolism</subject><subject>TOR Serine-Threonine Kinases - genetics</subject><subject>TOR Serine-Threonine Kinases - metabolism</subject><subject>Transcription Factor RelA - genetics</subject><subject>Transcription Factor RelA - metabolism</subject><subject>Valine - pharmacology</subject><subject>Valine deficiency</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO1DAQRSMEYoaBD2CDvBwWaew4rxYr1OIljcRmWFsVu5KuVmIH25lRfpmvwKF7WLIqq3zrlq9Plr0VfCe4qD-cdn2gXcFFuePNjnP5LLsWfF_l-33ZPN_OFc_LumyvslchnDjntaz5y-yqqNKMFOV19vv-iIymGcijYWQjhkgWRjYt2oVUaZoWiyysIeLEupU9wEipYbAnTWj1ynrn2eoWO7DBQwhMg5_Z7SGidfMR_GoHZ5xlZHAc4T2jwJLKaYKYVj5SPCYz7RECJYunfRHikoTWMI_DMkLcLiMNx8hOi9WRkuPsXUSygUUPNmhPc2TQLdaA1SmVZXELd8mEr7MXPYwB31zqTfbzy-f7w7f87sfX74dPd7mWlYx5r3vo2trIHruWgzQlR4nQS461MFKDlHKfOq1uQdelrnkjC96BaKAqyrKUN9nt2Tc979eSdquJgt6yW3RLUKKqipY3VdEkqThLtXcheOzV7GlKX6YEVxtidVIJsdoQK96ohDjNvLvYL92E5t_EE9Mk-HgWYAr5QOhV-AsKTWKsozKO_mP_B4qyv2I</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Luo, Jian-Bo</creator><creator>Feng, Lin</creator><creator>Jiang, Wei-Dan</creator><creator>Liu, Yang</creator><creator>Wu, Pei</creator><creator>Jiang, Jun</creator><creator>Kuang, Sheng-Yao</creator><creator>Tang, Ling</creator><creator>Zhang, Yong-An</creator><creator>Zhou, Xiao-Qiu</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140901</creationdate><title>The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine</title><author>Luo, Jian-Bo ; Feng, Lin ; Jiang, Wei-Dan ; Liu, Yang ; Wu, Pei ; Jiang, Jun ; Kuang, Sheng-Yao ; Tang, Ling ; Zhang, Yong-An ; Zhou, Xiao-Qiu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-fcfab86d3feb80a3d40e3eaf30e61d3ca3339e3e8c8ac64c607320ba17a524443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animal Feed - analysis</topic><topic>Animals</topic><topic>Carps - genetics</topic><topic>Carps - immunology</topic><topic>Diet - veterinary</topic><topic>Dietary Supplements - analysis</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Grass carp</topic><topic>I-kappa B Proteins - genetics</topic><topic>I-kappa B Proteins - metabolism</topic><topic>Immune status</topic><topic>Immunity, Mucosal - drug effects</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestine</topic><topic>NF-KappaB Inhibitor alpha</topic><topic>Tight junction proteins</topic><topic>Tight Junction Proteins - genetics</topic><topic>Tight Junction Proteins - metabolism</topic><topic>TOR Serine-Threonine Kinases - genetics</topic><topic>TOR Serine-Threonine Kinases - metabolism</topic><topic>Transcription Factor RelA - genetics</topic><topic>Transcription Factor RelA - metabolism</topic><topic>Valine - pharmacology</topic><topic>Valine deficiency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Jian-Bo</creatorcontrib><creatorcontrib>Feng, Lin</creatorcontrib><creatorcontrib>Jiang, Wei-Dan</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Wu, Pei</creatorcontrib><creatorcontrib>Jiang, Jun</creatorcontrib><creatorcontrib>Kuang, Sheng-Yao</creatorcontrib><creatorcontrib>Tang, Ling</creatorcontrib><creatorcontrib>Zhang, Yong-An</creatorcontrib><creatorcontrib>Zhou, Xiao-Qiu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Jian-Bo</au><au>Feng, Lin</au><au>Jiang, Wei-Dan</au><au>Liu, Yang</au><au>Wu, Pei</au><au>Jiang, Jun</au><au>Kuang, Sheng-Yao</au><au>Tang, Ling</au><au>Zhang, Yong-An</au><au>Zhou, Xiao-Qiu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>40</volume><issue>1</issue><spage>197</spage><epage>207</epage><pages>197-207</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract><![CDATA[This study investigated the effects of dietary valine on the growth, intestinal immune response, tight junction proteins transcript abundance and gene expression of immune-related signaling molecules in the intestine of young grass carp (Ctenopharyngodon idella). Six iso-nitrogenous diets containing graded levels of valine (4.3–19.1 g kg−1 diet) were fed to the fish for 8 weeks. The results showed that percentage weight gain (PWG), feed intake and feed efficiency of fish were the lowest in fish fed the valine-deficient diet (P < 0.05). In addition, valine deficiency decreased lysozyme, acid phosphatase activities and complement 3 content in the intestine (P < 0.05), down-regulated mRNA levels of interleukin 10, transforming growth factor β1, IκBα and target of rapamycin (TOR) (P < 0.05), and up-regulated tumor necrosis factor α, interleukin 8 and nuclear factor κB P65 (NF-κB P65) gene expression (P < 0.05). Additionally, valine deficiency significantly decreased transcript of Occludin, Claudin b, Claudin c, Claudin 3, and ZO-1 (P < 0.05), and improved Claudin 15 expression in the fish intestine (P < 0.05). However, valine did not have a significant effect on expression of Claudin 12 in the intestine of grass carp (P > 0.05). In conclusion, valine deficiency decreased fish growth and intestinal immune status, as well as regulated gene expression of tight junction proteins, NF-κB P65, IκBα and TOR in the fish intestine. Based on the quadratic regression analysis of lysozyme activity or PWG, the dietary valine requirement of young grass carp (268–679 g) were established to be 14.47 g kg−1 diet (4.82 g 100 g−1 CP) or 14.00 g kg−1 diet (4.77 g 100 g−1 CP), respectively.
•Valine deficiency decreased immune status of intestine.•Valine regulated the gene expression of NF-κB P65, IκBα, TOR.•Valine regulated tight junction proteins transcript abundance.]]></abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25014314</pmid><doi>10.1016/j.fsi.2014.07.003</doi><tpages>11</tpages></addata></record> |
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| ispartof | Fish & shellfish immunology, 2014-09, Vol.40 (1), p.197-207 |
| issn | 1050-4648 1095-9947 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal Feed - analysis Animals Carps - genetics Carps - immunology Diet - veterinary Dietary Supplements - analysis Fish Proteins - genetics Fish Proteins - metabolism Gene Expression Regulation Grass carp I-kappa B Proteins - genetics I-kappa B Proteins - metabolism Immune status Immunity, Mucosal - drug effects Intestinal Mucosa - drug effects Intestine NF-KappaB Inhibitor alpha Tight junction proteins Tight Junction Proteins - genetics Tight Junction Proteins - metabolism TOR Serine-Threonine Kinases - genetics TOR Serine-Threonine Kinases - metabolism Transcription Factor RelA - genetics Transcription Factor RelA - metabolism Valine - pharmacology Valine deficiency |
| title | The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine |
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