In vitro evidence that the pastoral Artemisia campestris species exerts an anthelmintic effect on Haemonchus contortus from sheep
Occurrence of anthelmintic resistant strains of helminths is increasing. The aim of this study was to evaluate the in vitro anthelmintic activity of Artemisia campestris in comparison to albendazole against Haemonchus contortus of sheep. In this respect, in vitro anthelmintic activities of crude aqu...
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| Veröffentlicht in: | Veterinary research communications 2014-09, Vol.38 (3), p.249-255 |
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| creator | Akkari, Hafidh Rtibi, Kais B’chir, Fatma Rekik, Mourad Darghouth, Mohamed Aziz Gharbi, Mohamed |
| description | Occurrence of anthelmintic resistant strains of helminths is increasing. The aim of this study was to evaluate the in vitro anthelmintic activity of Artemisia campestris in comparison to albendazole against Haemonchus contortus of sheep. In this respect, in vitro anthelmintic activities of crude aqueous and crude ethanolic extracts of aerial parts of A. campestris were investigated on eggs and adults of Haemonchus contortus. Chemical analyses revealed that overall profile of both extracts samples were dominated by flavonoids among them quercetin and apigenin derivatives were the most abundant phenolics constituents. Both extract types completely inhibited egg hatching at a concentration close to 2 mg/ml. Lethal concentration 50% of A. campestris ethanolic and aqueous extracts were 0.83 and 1.00 mg/ml respectively (p |
| doi_str_mv | 10.1007/s11259-014-9609-y |
| format | Article |
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The aim of this study was to evaluate the in vitro anthelmintic activity of Artemisia campestris in comparison to albendazole against Haemonchus contortus of sheep. In this respect, in vitro anthelmintic activities of crude aqueous and crude ethanolic extracts of aerial parts of A. campestris were investigated on eggs and adults of Haemonchus contortus. Chemical analyses revealed that overall profile of both extracts samples were dominated by flavonoids among them quercetin and apigenin derivatives were the most abundant phenolics constituents. Both extract types completely inhibited egg hatching at a concentration close to 2 mg/ml. Lethal concentration 50% of A. campestris ethanolic and aqueous extracts were 0.83 and 1.00 mg/ml respectively (p < 0.05). The ethanolic extract showed better in vitro activity against adult parasites than the aqueous extract in terms of the paralysis and/or death of the worms at different hours post-treatment. Dose dependent activity was also observed for both extract. After 8 and 24 h of exposure, the ethanolic extract induced 91.3 and 100% mortality at the highest tested concentration respectively, while the aqueous extract induced 3.22 and 70.96% at the same concentration respectively.To our knowledge, these results depict for the first time that A. campestris possesses in vitro anti-Haemonchus contortus properties.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-014-9609-y</identifier><identifier>PMID: 25027867</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>adults ; aerial parts ; albendazole ; Animals ; Anthelmintics - chemistry ; Anthelmintics - isolation & purification ; Anthelmintics - pharmacology ; apigenin ; Artemisia - chemistry ; Artemisia campestris ; Biomedical and Life Sciences ; chemical analysis ; death ; eggs ; Ethanol - chemistry ; Haemonchus - drug effects ; Haemonchus contortus ; hatching ; helminths ; Life Sciences ; mortality ; Motor Activity - drug effects ; paralysis ; phenolic compounds ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; quercetin ; Review Article ; Sheep ; Veterinary Medicine/Veterinary Science ; Water - chemistry ; Zoology ; Zygote - drug effects</subject><ispartof>Veterinary research communications, 2014-09, Vol.38 (3), p.249-255</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-b72236769fe02b5e8028d3f5e97ab5cc83520cd6a73f8c261299e3f87ad95b073</citedby><cites>FETCH-LOGICAL-c439t-b72236769fe02b5e8028d3f5e97ab5cc83520cd6a73f8c261299e3f87ad95b073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-014-9609-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-014-9609-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25027867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akkari, Hafidh</creatorcontrib><creatorcontrib>Rtibi, Kais</creatorcontrib><creatorcontrib>B’chir, Fatma</creatorcontrib><creatorcontrib>Rekik, Mourad</creatorcontrib><creatorcontrib>Darghouth, Mohamed Aziz</creatorcontrib><creatorcontrib>Gharbi, Mohamed</creatorcontrib><title>In vitro evidence that the pastoral Artemisia campestris species exerts an anthelmintic effect on Haemonchus contortus from sheep</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>Occurrence of anthelmintic resistant strains of helminths is increasing. The aim of this study was to evaluate the in vitro anthelmintic activity of Artemisia campestris in comparison to albendazole against Haemonchus contortus of sheep. In this respect, in vitro anthelmintic activities of crude aqueous and crude ethanolic extracts of aerial parts of A. campestris were investigated on eggs and adults of Haemonchus contortus. Chemical analyses revealed that overall profile of both extracts samples were dominated by flavonoids among them quercetin and apigenin derivatives were the most abundant phenolics constituents. Both extract types completely inhibited egg hatching at a concentration close to 2 mg/ml. Lethal concentration 50% of A. campestris ethanolic and aqueous extracts were 0.83 and 1.00 mg/ml respectively (p < 0.05). The ethanolic extract showed better in vitro activity against adult parasites than the aqueous extract in terms of the paralysis and/or death of the worms at different hours post-treatment. Dose dependent activity was also observed for both extract. After 8 and 24 h of exposure, the ethanolic extract induced 91.3 and 100% mortality at the highest tested concentration respectively, while the aqueous extract induced 3.22 and 70.96% at the same concentration respectively.To our knowledge, these results depict for the first time that A. campestris possesses in vitro anti-Haemonchus contortus properties.</description><subject>adults</subject><subject>aerial parts</subject><subject>albendazole</subject><subject>Animals</subject><subject>Anthelmintics - chemistry</subject><subject>Anthelmintics - isolation & purification</subject><subject>Anthelmintics - pharmacology</subject><subject>apigenin</subject><subject>Artemisia - chemistry</subject><subject>Artemisia campestris</subject><subject>Biomedical and Life Sciences</subject><subject>chemical analysis</subject><subject>death</subject><subject>eggs</subject><subject>Ethanol - chemistry</subject><subject>Haemonchus - drug effects</subject><subject>Haemonchus contortus</subject><subject>hatching</subject><subject>helminths</subject><subject>Life Sciences</subject><subject>mortality</subject><subject>Motor Activity - drug effects</subject><subject>paralysis</subject><subject>phenolic compounds</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>quercetin</subject><subject>Review Article</subject><subject>Sheep</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Water - chemistry</subject><subject>Zoology</subject><subject>Zygote - drug effects</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUFvFSEUhYnR2Gf1B7hREjdupl5gGIZl06ht0qQL7ZowzJ2-aWZgBKbxLf3n0k41xkUTAjfhOwdODiFvGZwwAPUpMcalroDVlW5AV4dnZMekEpWq6-Y52QFrZKVEC0fkVUq3AKBbEC_JEZfAVduoHfl14endmGOgeDf26B3SvLe5bEgXm3KIdqKnMeM8ptFSZ-cFU45jomlBN2Ki-BNjTtT6sopqmkefR0dxGNBlGjw9tzgH7_Zroi744pjLNMQw07RHXF6TF4OdEr55PI_J9ZfP38_Oq8urrxdnp5eVq4XOVac4F41q9IDAO4kt8LYXg0StbCeda4Xk4PrGKjG0jjeMa41lVLbXsgMljsnHzXeJ4cdaQpgSyeE0WY9hTYZJyYWqgcuCfvgPvQ1r9OV3DxQDWeumUGyjXAwpRRzMEsfZxoNhYO77MVs_pvRj7vsxh6J59-i8djP2fxV_CikA34BUrvwNxn-efsL1_SYabDD2prRjrr_xAgCwEooL8Rv7JaZL</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Akkari, Hafidh</creator><creator>Rtibi, Kais</creator><creator>B’chir, Fatma</creator><creator>Rekik, Mourad</creator><creator>Darghouth, Mohamed Aziz</creator><creator>Gharbi, Mohamed</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20140901</creationdate><title>In vitro evidence that the pastoral Artemisia campestris species exerts an anthelmintic effect on Haemonchus contortus from sheep</title><author>Akkari, Hafidh ; 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The aim of this study was to evaluate the in vitro anthelmintic activity of Artemisia campestris in comparison to albendazole against Haemonchus contortus of sheep. In this respect, in vitro anthelmintic activities of crude aqueous and crude ethanolic extracts of aerial parts of A. campestris were investigated on eggs and adults of Haemonchus contortus. Chemical analyses revealed that overall profile of both extracts samples were dominated by flavonoids among them quercetin and apigenin derivatives were the most abundant phenolics constituents. Both extract types completely inhibited egg hatching at a concentration close to 2 mg/ml. Lethal concentration 50% of A. campestris ethanolic and aqueous extracts were 0.83 and 1.00 mg/ml respectively (p < 0.05). The ethanolic extract showed better in vitro activity against adult parasites than the aqueous extract in terms of the paralysis and/or death of the worms at different hours post-treatment. Dose dependent activity was also observed for both extract. After 8 and 24 h of exposure, the ethanolic extract induced 91.3 and 100% mortality at the highest tested concentration respectively, while the aqueous extract induced 3.22 and 70.96% at the same concentration respectively.To our knowledge, these results depict for the first time that A. campestris possesses in vitro anti-Haemonchus contortus properties.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>25027867</pmid><doi>10.1007/s11259-014-9609-y</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Veterinary research communications, 2014-09, Vol.38 (3), p.249-255 |
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| subjects | adults aerial parts albendazole Animals Anthelmintics - chemistry Anthelmintics - isolation & purification Anthelmintics - pharmacology apigenin Artemisia - chemistry Artemisia campestris Biomedical and Life Sciences chemical analysis death eggs Ethanol - chemistry Haemonchus - drug effects Haemonchus contortus hatching helminths Life Sciences mortality Motor Activity - drug effects paralysis phenolic compounds Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology quercetin Review Article Sheep Veterinary Medicine/Veterinary Science Water - chemistry Zoology Zygote - drug effects |
| title | In vitro evidence that the pastoral Artemisia campestris species exerts an anthelmintic effect on Haemonchus contortus from sheep |
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