Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant
Nitrous oxide (N2O) production and expression of genes capable of its reduction were investigated in two full-scale parallel plug-flow activated sludge systems. These two systems continuously received wastewater with the same constituents, but operated under distinct nitrification efficiencies due t...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2014-09, Vol.118 (3), p.289-297 |
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| creator | Song, Kang Suenaga, Toshikazu Hamamoto, Aki Satou, Kouichi Riya, Shohei Hosomi, Masaaki Terada, Akihiko |
| description | Nitrous oxide (N2O) production and expression of genes capable of its reduction were investigated in two full-scale parallel plug-flow activated sludge systems. These two systems continuously received wastewater with the same constituents, but operated under distinct nitrification efficiencies due to mixed liquor suspended solid (MLSS) concentration and the different hydraulic retention times (HRTs). A shorter HRT in system 2 resulted in a lower nitrification efficiency (40–60%) in conjunction with a high N2O emission (50.6 mg-N/L/day), whereas there was a higher nitrification efficiency (>99%) in system 1 with low N2O emission (22.6 mg-N/L/day). The DNA abundance of functional genes responsible for nitrification and denitrification were comparable in both systems, but transcription of nosZ mRNA in the lower N2O emission system (system 1) was one order of magnitude higher than that in the higher N2O emission system (system 2). The diversity and evenness of the nosZ gene were nearly identical; however, the predominant N2O reducing bacteria were phylogenetically distinct. Phylogenetic analysis indicated that N2O-reducing strains only retrieved in system 1 were close to the genera Rhodobacter, Oligotropha and Shinella, whereas they were close to the genera Mesorhizobium only in system 2. The distinct predominant N2O reducers may directly or indirectly influence N2O emissions. |
| doi_str_mv | 10.1016/j.jbiosc.2014.02.028 |
| format | Article |
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These two systems continuously received wastewater with the same constituents, but operated under distinct nitrification efficiencies due to mixed liquor suspended solid (MLSS) concentration and the different hydraulic retention times (HRTs). A shorter HRT in system 2 resulted in a lower nitrification efficiency (40–60%) in conjunction with a high N2O emission (50.6 mg-N/L/day), whereas there was a higher nitrification efficiency (>99%) in system 1 with low N2O emission (22.6 mg-N/L/day). The DNA abundance of functional genes responsible for nitrification and denitrification were comparable in both systems, but transcription of nosZ mRNA in the lower N2O emission system (system 1) was one order of magnitude higher than that in the higher N2O emission system (system 2). The diversity and evenness of the nosZ gene were nearly identical; however, the predominant N2O reducing bacteria were phylogenetically distinct. Phylogenetic analysis indicated that N2O-reducing strains only retrieved in system 1 were close to the genera Rhodobacter, Oligotropha and Shinella, whereas they were close to the genera Mesorhizobium only in system 2. The distinct predominant N2O reducers may directly or indirectly influence N2O emissions.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2014.02.028</identifier><identifier>PMID: 24725963</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Abundance of DNA and mRNA ; Bacteria - classification ; Bacteria - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biological and medical sciences ; Biological treatment of waters ; Bioreactors ; Biotechnology ; Denitrification ; Environment and pollution ; Fundamental and applied biological sciences. Psychology ; Gene transcription ; Industrial applications and implications. Economical aspects ; N2O reductase gene (nosZ) ; N2O reduction bacteria ; Nitrification ; Nitrous Oxide - metabolism ; Nitrous oxide reduction ; Oxidation-Reduction ; Phylogeny ; Transcription, Genetic ; Waste Disposal, Fluid ; Waste Water - microbiology ; Wastewater treatment plants</subject><ispartof>Journal of bioscience and bioengineering, 2014-09, Vol.118 (3), p.289-297</ispartof><rights>2014 The Society for Biotechnology, Japan</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-fe7212e8090c2b51d16df50d6d3e9b6e39fa733c0560773ab56c7213461890263</citedby><cites>FETCH-LOGICAL-c555t-fe7212e8090c2b51d16df50d6d3e9b6e39fa733c0560773ab56c7213461890263</cites><orcidid>0000-0002-9258-6912</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiosc.2014.02.028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28778587$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24725963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Kang</creatorcontrib><creatorcontrib>Suenaga, Toshikazu</creatorcontrib><creatorcontrib>Hamamoto, Aki</creatorcontrib><creatorcontrib>Satou, Kouichi</creatorcontrib><creatorcontrib>Riya, Shohei</creatorcontrib><creatorcontrib>Hosomi, Masaaki</creatorcontrib><creatorcontrib>Terada, Akihiko</creatorcontrib><title>Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>Nitrous oxide (N2O) production and expression of genes capable of its reduction were investigated in two full-scale parallel plug-flow activated sludge systems. These two systems continuously received wastewater with the same constituents, but operated under distinct nitrification efficiencies due to mixed liquor suspended solid (MLSS) concentration and the different hydraulic retention times (HRTs). A shorter HRT in system 2 resulted in a lower nitrification efficiency (40–60%) in conjunction with a high N2O emission (50.6 mg-N/L/day), whereas there was a higher nitrification efficiency (>99%) in system 1 with low N2O emission (22.6 mg-N/L/day). The DNA abundance of functional genes responsible for nitrification and denitrification were comparable in both systems, but transcription of nosZ mRNA in the lower N2O emission system (system 1) was one order of magnitude higher than that in the higher N2O emission system (system 2). The diversity and evenness of the nosZ gene were nearly identical; however, the predominant N2O reducing bacteria were phylogenetically distinct. Phylogenetic analysis indicated that N2O-reducing strains only retrieved in system 1 were close to the genera Rhodobacter, Oligotropha and Shinella, whereas they were close to the genera Mesorhizobium only in system 2. The distinct predominant N2O reducers may directly or indirectly influence N2O emissions.</description><subject>Abundance of DNA and mRNA</subject><subject>Bacteria - classification</subject><subject>Bacteria - metabolism</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Denitrification</subject><subject>Environment and pollution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene transcription</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>N2O reductase gene (nosZ)</subject><subject>N2O reduction bacteria</subject><subject>Nitrification</subject><subject>Nitrous Oxide - metabolism</subject><subject>Nitrous oxide reduction</subject><subject>Oxidation-Reduction</subject><subject>Phylogeny</subject><subject>Transcription, Genetic</subject><subject>Waste Disposal, Fluid</subject><subject>Waste Water - microbiology</subject><subject>Wastewater treatment plants</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhiNERT_gHyDkCxIHsvgjjpMLUlVBQarUSzlbE3sCXjlOsJ22-wf43fWyC9yQRpqR9bwz43mr6jWjG0ZZ-2G72Q5uTmbDKWs2lJfonlVnTDSqbhrOnu_rrq-Z4uK0Ok9pSylTVLEX1SlvFJd9K86qX5fDGiwEg-9JjhCSiW7Jbg7E4z36RCBYsvzY-fk7hh2ZRzKAyRgdEAMLDB73b8HlOK-JzI_OIoloV_O7hwsEyLQGZ9wCnjxAyvgARV5mIeQJQyaLh5BfVicj-ISvjvmi-vb5093Vl_rm9vrr1eVNbaSUuR5Rccaxoz01fJDMstaOktrWCuyHFkU_ghLCUNlSpQQMsjVFIZqWdT3lrbio3h36LnH-uWLKenLJoC87YPmAZlJyoVghC9ocUBPnlCKOeolugrjTjOq9A3qrDw7ovQOa8hJdkb05TliHCe1f0Z-TF-DtEYBkwI_l6Malf1ynVCc7VbiPB664gPcOo07GYTHKuogmazu7_2_yBEPuqAc</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Song, Kang</creator><creator>Suenaga, Toshikazu</creator><creator>Hamamoto, Aki</creator><creator>Satou, Kouichi</creator><creator>Riya, Shohei</creator><creator>Hosomi, Masaaki</creator><creator>Terada, Akihiko</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9258-6912</orcidid></search><sort><creationdate>20140901</creationdate><title>Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant</title><author>Song, Kang ; Suenaga, Toshikazu ; Hamamoto, Aki ; Satou, Kouichi ; Riya, Shohei ; Hosomi, Masaaki ; Terada, Akihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-fe7212e8090c2b51d16df50d6d3e9b6e39fa733c0560773ab56c7213461890263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Abundance of DNA and mRNA</topic><topic>Bacteria - classification</topic><topic>Bacteria - metabolism</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biological treatment of waters</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Denitrification</topic><topic>Environment and pollution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene transcription</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>N2O reductase gene (nosZ)</topic><topic>N2O reduction bacteria</topic><topic>Nitrification</topic><topic>Nitrous Oxide - metabolism</topic><topic>Nitrous oxide reduction</topic><topic>Oxidation-Reduction</topic><topic>Phylogeny</topic><topic>Transcription, Genetic</topic><topic>Waste Disposal, Fluid</topic><topic>Waste Water - microbiology</topic><topic>Wastewater treatment plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Kang</creatorcontrib><creatorcontrib>Suenaga, Toshikazu</creatorcontrib><creatorcontrib>Hamamoto, Aki</creatorcontrib><creatorcontrib>Satou, Kouichi</creatorcontrib><creatorcontrib>Riya, Shohei</creatorcontrib><creatorcontrib>Hosomi, Masaaki</creatorcontrib><creatorcontrib>Terada, Akihiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Kang</au><au>Suenaga, Toshikazu</au><au>Hamamoto, Aki</au><au>Satou, Kouichi</au><au>Riya, Shohei</au><au>Hosomi, Masaaki</au><au>Terada, Akihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>118</volume><issue>3</issue><spage>289</spage><epage>297</epage><pages>289-297</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Nitrous oxide (N2O) production and expression of genes capable of its reduction were investigated in two full-scale parallel plug-flow activated sludge systems. These two systems continuously received wastewater with the same constituents, but operated under distinct nitrification efficiencies due to mixed liquor suspended solid (MLSS) concentration and the different hydraulic retention times (HRTs). A shorter HRT in system 2 resulted in a lower nitrification efficiency (40–60%) in conjunction with a high N2O emission (50.6 mg-N/L/day), whereas there was a higher nitrification efficiency (>99%) in system 1 with low N2O emission (22.6 mg-N/L/day). The DNA abundance of functional genes responsible for nitrification and denitrification were comparable in both systems, but transcription of nosZ mRNA in the lower N2O emission system (system 1) was one order of magnitude higher than that in the higher N2O emission system (system 2). The diversity and evenness of the nosZ gene were nearly identical; however, the predominant N2O reducing bacteria were phylogenetically distinct. Phylogenetic analysis indicated that N2O-reducing strains only retrieved in system 1 were close to the genera Rhodobacter, Oligotropha and Shinella, whereas they were close to the genera Mesorhizobium only in system 2. The distinct predominant N2O reducers may directly or indirectly influence N2O emissions.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>24725963</pmid><doi>10.1016/j.jbiosc.2014.02.028</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9258-6912</orcidid></addata></record> |
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| ispartof | Journal of bioscience and bioengineering, 2014-09, Vol.118 (3), p.289-297 |
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| language | eng |
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| subjects | Abundance of DNA and mRNA Bacteria - classification Bacteria - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Biological treatment of waters Bioreactors Biotechnology Denitrification Environment and pollution Fundamental and applied biological sciences. Psychology Gene transcription Industrial applications and implications. Economical aspects N2O reductase gene (nosZ) N2O reduction bacteria Nitrification Nitrous Oxide - metabolism Nitrous oxide reduction Oxidation-Reduction Phylogeny Transcription, Genetic Waste Disposal, Fluid Waste Water - microbiology Wastewater treatment plants |
| title | Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant |
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