Expression pattern and first functional characterization of riok-1 in Caenorhabditis elegans
•riok-1 is expressed in the neurons, the vulva, the uterus and the spermatheca.•riok-1 and let-60 share spatial and temporal expression patterns.•riok-1 expression is essential for proper gonad development of C. elegans.•riok-1 is a novel modulator of Ras signaling in C. elegans. Rio kinases are aty...
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| Veröffentlicht in: | Gene Expression Patterns 2014-07, Vol.15 (2), p.124-134 |
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| creator | Weinberg, Florian Schulze, Ekkehard Fatouros, Chronis Schmidt, Enrico Baumeister, Ralf Brummer, Tilman |
| description | •riok-1 is expressed in the neurons, the vulva, the uterus and the spermatheca.•riok-1 and let-60 share spatial and temporal expression patterns.•riok-1 expression is essential for proper gonad development of C. elegans.•riok-1 is a novel modulator of Ras signaling in C. elegans.
Rio kinases are atypical serine/threonine kinases that emerge as potential cooperation partners in Ras-driven tumors. In the current study, we performed an RNAi screen in Caenorhabditis elegans to identify suppressors of oncogenic Ras signaling. Aberrant Ras/Raf signaling in C. elegans leads to the formation of a multi-vulva (Muv) phenotype. We found that depletion of riok-1, the C. elegans orthologue of the mammalian RioK1, suppressed the Muv phenotype. By using a promoter GFP construct, we could show that riok-1 is expressed in neuronal cells, the somatic gonad, the vulva, the uterus and the spermatheca. Furthermore, we observed developmental defects in the gonad upon riok-1 knockdown in a wildtype background. Our data suggest that riok-1 is a modulator of the Ras signaling pathway, suggesting implications for novel interventions in the context of Ras-driven tumors. |
| doi_str_mv | 10.1016/j.gep.2014.05.005 |
| format | Article |
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Rio kinases are atypical serine/threonine kinases that emerge as potential cooperation partners in Ras-driven tumors. In the current study, we performed an RNAi screen in Caenorhabditis elegans to identify suppressors of oncogenic Ras signaling. Aberrant Ras/Raf signaling in C. elegans leads to the formation of a multi-vulva (Muv) phenotype. We found that depletion of riok-1, the C. elegans orthologue of the mammalian RioK1, suppressed the Muv phenotype. By using a promoter GFP construct, we could show that riok-1 is expressed in neuronal cells, the somatic gonad, the vulva, the uterus and the spermatheca. Furthermore, we observed developmental defects in the gonad upon riok-1 knockdown in a wildtype background. Our data suggest that riok-1 is a modulator of the Ras signaling pathway, suggesting implications for novel interventions in the context of Ras-driven tumors.</description><identifier>ISSN: 1567-133X</identifier><identifier>EISSN: 1872-7298</identifier><identifier>DOI: 10.1016/j.gep.2014.05.005</identifier><identifier>PMID: 24929033</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Butadienes - chemistry ; Caenorhabditis elegans - embryology ; Caenorhabditis elegans Proteins - metabolism ; Cell Lineage ; Enzyme Inhibitors - chemistry ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Gonad development ; Gonads - embryology ; LET-60 ; Molecular Sequence Data ; Multi-vulva phenotype ; Neurons - metabolism ; Nitriles - chemistry ; Phenotype ; Protein-Serine-Threonine Kinases - metabolism ; RIOK-1 ; RNA Interference ; RNAi screen ; Signal Transduction ; Time Factors ; Vulval development</subject><ispartof>Gene Expression Patterns, 2014-07, Vol.15 (2), p.124-134</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-214e9e720ba191cf462014f5e88fc089fb183c5d186329b72210ee1f2742f1b63</citedby><cites>FETCH-LOGICAL-c353t-214e9e720ba191cf462014f5e88fc089fb183c5d186329b72210ee1f2742f1b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gep.2014.05.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24929033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weinberg, Florian</creatorcontrib><creatorcontrib>Schulze, Ekkehard</creatorcontrib><creatorcontrib>Fatouros, Chronis</creatorcontrib><creatorcontrib>Schmidt, Enrico</creatorcontrib><creatorcontrib>Baumeister, Ralf</creatorcontrib><creatorcontrib>Brummer, Tilman</creatorcontrib><title>Expression pattern and first functional characterization of riok-1 in Caenorhabditis elegans</title><title>Gene Expression Patterns</title><addtitle>Gene Expr Patterns</addtitle><description>•riok-1 is expressed in the neurons, the vulva, the uterus and the spermatheca.•riok-1 and let-60 share spatial and temporal expression patterns.•riok-1 expression is essential for proper gonad development of C. elegans.•riok-1 is a novel modulator of Ras signaling in C. elegans.
Rio kinases are atypical serine/threonine kinases that emerge as potential cooperation partners in Ras-driven tumors. In the current study, we performed an RNAi screen in Caenorhabditis elegans to identify suppressors of oncogenic Ras signaling. Aberrant Ras/Raf signaling in C. elegans leads to the formation of a multi-vulva (Muv) phenotype. We found that depletion of riok-1, the C. elegans orthologue of the mammalian RioK1, suppressed the Muv phenotype. By using a promoter GFP construct, we could show that riok-1 is expressed in neuronal cells, the somatic gonad, the vulva, the uterus and the spermatheca. Furthermore, we observed developmental defects in the gonad upon riok-1 knockdown in a wildtype background. Our data suggest that riok-1 is a modulator of the Ras signaling pathway, suggesting implications for novel interventions in the context of Ras-driven tumors.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Butadienes - chemistry</subject><subject>Caenorhabditis elegans - embryology</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>Cell Lineage</subject><subject>Enzyme Inhibitors - chemistry</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gonad development</subject><subject>Gonads - embryology</subject><subject>LET-60</subject><subject>Molecular Sequence Data</subject><subject>Multi-vulva phenotype</subject><subject>Neurons - metabolism</subject><subject>Nitriles - chemistry</subject><subject>Phenotype</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>RIOK-1</subject><subject>RNA Interference</subject><subject>RNAi screen</subject><subject>Signal Transduction</subject><subject>Time Factors</subject><subject>Vulval development</subject><issn>1567-133X</issn><issn>1872-7298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UE1P3DAQtSpQ-Wh_QC-Vj1wSPHac2OoJrWhBQuJCJQ6VLMcZg7dZJ9jZivbX49VSjpxmNO9D8x4hX4DVwKA9X9cPONecQVMzWTMmP5BjUB2vOq7VQdll21UgxP0ROcl5zYpGt_ojOeKN5poJcUx-XT7PCXMOU6SzXRZMkdo4UB9SXqjfRrcUyI7UPdpkXcHDP7s70cnTFKbfFdAQ6cpinNKj7YewhExxxAcb8ydy6O2Y8fPrPCU_v1_era6qm9sf16uLm8oJKZaKQ4MaO856Cxqcb9pdJC9RKe-Y0r4HJZwcQLWC677jHBgieN413EPfilNytved0_S0xbyYTcgOx9FGnLbZgJSgQCnOChX2VJemnBN6M6ewsemvAWZ2pZq1KaWa3QeGSVNKLZqvr_bbfoPDm-J_i4XwbU_AEvJPwGSyCxgdDiGhW8wwhXfsXwBg7Ie9</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Weinberg, Florian</creator><creator>Schulze, Ekkehard</creator><creator>Fatouros, Chronis</creator><creator>Schmidt, Enrico</creator><creator>Baumeister, Ralf</creator><creator>Brummer, Tilman</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140701</creationdate><title>Expression pattern and first functional characterization of riok-1 in Caenorhabditis elegans</title><author>Weinberg, Florian ; Schulze, Ekkehard ; Fatouros, Chronis ; Schmidt, Enrico ; Baumeister, Ralf ; Brummer, Tilman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-214e9e720ba191cf462014f5e88fc089fb183c5d186329b72210ee1f2742f1b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Butadienes - chemistry</topic><topic>Caenorhabditis elegans - embryology</topic><topic>Caenorhabditis elegans Proteins - metabolism</topic><topic>Cell Lineage</topic><topic>Enzyme Inhibitors - chemistry</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gonad development</topic><topic>Gonads - embryology</topic><topic>LET-60</topic><topic>Molecular Sequence Data</topic><topic>Multi-vulva phenotype</topic><topic>Neurons - metabolism</topic><topic>Nitriles - chemistry</topic><topic>Phenotype</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>RIOK-1</topic><topic>RNA Interference</topic><topic>RNAi screen</topic><topic>Signal Transduction</topic><topic>Time Factors</topic><topic>Vulval development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weinberg, Florian</creatorcontrib><creatorcontrib>Schulze, Ekkehard</creatorcontrib><creatorcontrib>Fatouros, Chronis</creatorcontrib><creatorcontrib>Schmidt, Enrico</creatorcontrib><creatorcontrib>Baumeister, Ralf</creatorcontrib><creatorcontrib>Brummer, Tilman</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene Expression Patterns</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weinberg, Florian</au><au>Schulze, Ekkehard</au><au>Fatouros, Chronis</au><au>Schmidt, Enrico</au><au>Baumeister, Ralf</au><au>Brummer, Tilman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression pattern and first functional characterization of riok-1 in Caenorhabditis elegans</atitle><jtitle>Gene Expression Patterns</jtitle><addtitle>Gene Expr Patterns</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>15</volume><issue>2</issue><spage>124</spage><epage>134</epage><pages>124-134</pages><issn>1567-133X</issn><eissn>1872-7298</eissn><abstract>•riok-1 is expressed in the neurons, the vulva, the uterus and the spermatheca.•riok-1 and let-60 share spatial and temporal expression patterns.•riok-1 expression is essential for proper gonad development of C. elegans.•riok-1 is a novel modulator of Ras signaling in C. elegans.
Rio kinases are atypical serine/threonine kinases that emerge as potential cooperation partners in Ras-driven tumors. In the current study, we performed an RNAi screen in Caenorhabditis elegans to identify suppressors of oncogenic Ras signaling. Aberrant Ras/Raf signaling in C. elegans leads to the formation of a multi-vulva (Muv) phenotype. We found that depletion of riok-1, the C. elegans orthologue of the mammalian RioK1, suppressed the Muv phenotype. By using a promoter GFP construct, we could show that riok-1 is expressed in neuronal cells, the somatic gonad, the vulva, the uterus and the spermatheca. Furthermore, we observed developmental defects in the gonad upon riok-1 knockdown in a wildtype background. Our data suggest that riok-1 is a modulator of the Ras signaling pathway, suggesting implications for novel interventions in the context of Ras-driven tumors.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24929033</pmid><doi>10.1016/j.gep.2014.05.005</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Base Sequence Butadienes - chemistry Caenorhabditis elegans - embryology Caenorhabditis elegans Proteins - metabolism Cell Lineage Enzyme Inhibitors - chemistry Extracellular Signal-Regulated MAP Kinases - metabolism Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Gonad development Gonads - embryology LET-60 Molecular Sequence Data Multi-vulva phenotype Neurons - metabolism Nitriles - chemistry Phenotype Protein-Serine-Threonine Kinases - metabolism RIOK-1 RNA Interference RNAi screen Signal Transduction Time Factors Vulval development |
| title | Expression pattern and first functional characterization of riok-1 in Caenorhabditis elegans |
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