Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein
Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E(123S)); however, JEV with an asparagine residue (E(123N)) has also been isolated. To compare the g...
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| Veröffentlicht in: | Journal of general virology 2013-01, Vol.94 (Pt 1), p.90-96 |
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| container_title | Journal of general virology |
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| creator | Yamaguchi, Yukie Nukui, Yoko Kotaki, Akira Sawabe, Kyoko Saijo, Masayuki Watanabe, Haruo Kurane, Ichiro Takasaki, Tomohiko Tajima, Shigeru |
| description | Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E(123S)); however, JEV with an asparagine residue (E(123N)) has also been isolated. To compare the growth properties and pathogenicity of E(123S) and E(123N) JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-E(S123N)) in the E(123S)-type strain Mie/41/2002 background. The growth rate of rJEV-Mie41-E(S123N) was similar to that of Mie/41/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie/41/2002) and rJEV-Mie41-E(S123N). Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo. |
| doi_str_mv | 10.1099/vir.0.044925-0 |
| format | Article |
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The majority of JEV strains have a serine residue at this position (E(123S)); however, JEV with an asparagine residue (E(123N)) has also been isolated. To compare the growth properties and pathogenicity of E(123S) and E(123N) JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-E(S123N)) in the E(123S)-type strain Mie/41/2002 background. The growth rate of rJEV-Mie41-E(S123N) was similar to that of Mie/41/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie/41/2002) and rJEV-Mie41-E(S123N). Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo.</description><identifier>ISSN: 0022-1317</identifier><identifier>ISSN: 1465-2099</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.044925-0</identifier><identifier>PMID: 23052392</identifier><language>eng</language><publisher>England</publisher><subject>Amino Acid Substitution ; Animals ; Asparagine - genetics ; Asparagine - metabolism ; Cell Line ; Culicidae - virology ; Encephalitis Virus, Japanese - genetics ; Encephalitis Virus, Japanese - growth & development ; Encephalitis Virus, Japanese - metabolism ; Female ; Japanese encephalitis virus ; Mice ; Phylogeny ; Serine - genetics ; Serine - metabolism ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - metabolism</subject><ispartof>Journal of general virology, 2013-01, Vol.94 (Pt 1), p.90-96</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-130b648c03db8ce7383abd8546f73494002c6799ecb28c83e0c5d5849cd000d03</citedby><cites>FETCH-LOGICAL-c434t-130b648c03db8ce7383abd8546f73494002c6799ecb28c83e0c5d5849cd000d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,3750,27933,27934</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23052392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamaguchi, Yukie</creatorcontrib><creatorcontrib>Nukui, Yoko</creatorcontrib><creatorcontrib>Kotaki, Akira</creatorcontrib><creatorcontrib>Sawabe, Kyoko</creatorcontrib><creatorcontrib>Saijo, Masayuki</creatorcontrib><creatorcontrib>Watanabe, Haruo</creatorcontrib><creatorcontrib>Kurane, Ichiro</creatorcontrib><creatorcontrib>Takasaki, Tomohiko</creatorcontrib><creatorcontrib>Tajima, Shigeru</creatorcontrib><title>Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E(123S)); however, JEV with an asparagine residue (E(123N)) has also been isolated. To compare the growth properties and pathogenicity of E(123S) and E(123N) JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-E(S123N)) in the E(123S)-type strain Mie/41/2002 background. The growth rate of rJEV-Mie41-E(S123N) was similar to that of Mie/41/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie/41/2002) and rJEV-Mie41-E(S123N). Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo.</description><subject>Amino Acid Substitution</subject><subject>Animals</subject><subject>Asparagine - genetics</subject><subject>Asparagine - metabolism</subject><subject>Cell Line</subject><subject>Culicidae - virology</subject><subject>Encephalitis Virus, Japanese - genetics</subject><subject>Encephalitis Virus, Japanese - growth & development</subject><subject>Encephalitis Virus, Japanese - metabolism</subject><subject>Female</subject><subject>Japanese encephalitis virus</subject><subject>Mice</subject><subject>Phylogeny</subject><subject>Serine - genetics</subject><subject>Serine - metabolism</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - metabolism</subject><issn>0022-1317</issn><issn>1465-2099</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctPwzAMxiMEYmNw5Yhy5NLhvNrmiKbx0iQucI7SNGVBXVuSFAn-erIHXDnZln-yP_tD6JLAnICUN5_Oz2EOnEsqMjhCU8JzkdHUOkZTAEozwkgxQWchvAMQzkVxiiaUgaBM0imKi7X22kTr3beOru9w32CNQ6o7m8U-02FIwFuqcBirEF0cd5iOeOiD2-WEMuw6HNcWP-lBdzZYbDtjh7VuExFwUjkGvMSD76N13Tk6aXQb7MUhztDr3fJl8ZCtnu8fF7erzHDGY1IOVc5LA6yuSmMLVjJd1aXgeVMwLnk6z-SFlNZUtDQls2BELUouTQ0ANbAZut7PTXs_Rhui2rhgbNsmif0YFBGC5Dy9Qv6PpndBTkiKMzTfo8b3IXjbqMG7jfZfioDamqLSuQrU3hS1lXF1mD1WG1v_4b8usB-DZIhS</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Yamaguchi, Yukie</creator><creator>Nukui, Yoko</creator><creator>Kotaki, Akira</creator><creator>Sawabe, Kyoko</creator><creator>Saijo, Masayuki</creator><creator>Watanabe, Haruo</creator><creator>Kurane, Ichiro</creator><creator>Takasaki, Tomohiko</creator><creator>Tajima, Shigeru</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>201301</creationdate><title>Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein</title><author>Yamaguchi, Yukie ; Nukui, Yoko ; Kotaki, Akira ; Sawabe, Kyoko ; Saijo, Masayuki ; Watanabe, Haruo ; Kurane, Ichiro ; Takasaki, Tomohiko ; Tajima, Shigeru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-130b648c03db8ce7383abd8546f73494002c6799ecb28c83e0c5d5849cd000d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino Acid Substitution</topic><topic>Animals</topic><topic>Asparagine - genetics</topic><topic>Asparagine - metabolism</topic><topic>Cell Line</topic><topic>Culicidae - virology</topic><topic>Encephalitis Virus, Japanese - genetics</topic><topic>Encephalitis Virus, Japanese - growth & development</topic><topic>Encephalitis Virus, Japanese - metabolism</topic><topic>Female</topic><topic>Japanese encephalitis virus</topic><topic>Mice</topic><topic>Phylogeny</topic><topic>Serine - genetics</topic><topic>Serine - metabolism</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamaguchi, Yukie</creatorcontrib><creatorcontrib>Nukui, Yoko</creatorcontrib><creatorcontrib>Kotaki, Akira</creatorcontrib><creatorcontrib>Sawabe, Kyoko</creatorcontrib><creatorcontrib>Saijo, Masayuki</creatorcontrib><creatorcontrib>Watanabe, Haruo</creatorcontrib><creatorcontrib>Kurane, Ichiro</creatorcontrib><creatorcontrib>Takasaki, Tomohiko</creatorcontrib><creatorcontrib>Tajima, Shigeru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamaguchi, Yukie</au><au>Nukui, Yoko</au><au>Kotaki, Akira</au><au>Sawabe, Kyoko</au><au>Saijo, Masayuki</au><au>Watanabe, Haruo</au><au>Kurane, Ichiro</au><au>Takasaki, Tomohiko</au><au>Tajima, Shigeru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2013-01</date><risdate>2013</risdate><volume>94</volume><issue>Pt 1</issue><spage>90</spage><epage>96</epage><pages>90-96</pages><issn>0022-1317</issn><issn>1465-2099</issn><eissn>1465-2099</eissn><abstract>Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E(123S)); however, JEV with an asparagine residue (E(123N)) has also been isolated. To compare the growth properties and pathogenicity of E(123S) and E(123N) JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-E(S123N)) in the E(123S)-type strain Mie/41/2002 background. The growth rate of rJEV-Mie41-E(S123N) was similar to that of Mie/41/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie/41/2002) and rJEV-Mie41-E(S123N). Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo.</abstract><cop>England</cop><pmid>23052392</pmid><doi>10.1099/vir.0.044925-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Substitution Animals Asparagine - genetics Asparagine - metabolism Cell Line Culicidae - virology Encephalitis Virus, Japanese - genetics Encephalitis Virus, Japanese - growth & development Encephalitis Virus, Japanese - metabolism Female Japanese encephalitis virus Mice Phylogeny Serine - genetics Serine - metabolism Viral Envelope Proteins - genetics Viral Envelope Proteins - metabolism |
| title | Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein |
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