Antigenic diversity and cross-reactivity of avian influenza H5N1 viruses in Egypt between 2006 and 2011
Influenza epidemics are a major health concern worldwide. Highly pathogenic avian influenza (HPAI) H5N1 viruses in Egypt have been subject to rapid genetic and antigenic changes since the first outbreak in February 2006 and have been endemic in poultry in Egypt since 2008. In this study, 33 H5N1 vir...
Gespeichert in:
| Veröffentlicht in: | Journal of general virology 2012-12, Vol.93 (Pt 12), p.2564-2574 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2574 |
|---|---|
| container_issue | Pt 12 |
| container_start_page | 2564 |
| container_title | Journal of general virology |
| container_volume | 93 |
| creator | EL-SHESHENY, Rabeh KAYALI, Ghazi KANDEIL, Ahmed ZHIPENG CAI BARAKAT, Ahmed B GHANIM, Hossam ALI, Mohamed A |
| description | Influenza epidemics are a major health concern worldwide. Highly pathogenic avian influenza (HPAI) H5N1 viruses in Egypt have been subject to rapid genetic and antigenic changes since the first outbreak in February 2006 and have been endemic in poultry in Egypt since 2008. In this study, 33 H5N1 viruses isolated from avian hosts were antigenically analysed by using a panel of eight mAbs raised against the A/Viet Nam/1203/04 (H5N1; clade 1) and A/bar-headed goose/Qinghai-lake/1A/05 (H5N1; clade 2.2) influenza viruses. Rats were immunized with inactivated whole-virus vaccine produced by reverse genetics with the haemagglutinin and neuraminidase genes of eight antigenically different HPAI H5N1 virus isolates and six internal genes from A/Puerto Rico/8/1934 (PR8) to produce polyclonal antibodies. Cross-reactivity between the obtained polyclonal antibodies and the isolated viruses was assayed. Antigenic cartography of the isolated viruses showed that three antigenic clusters were defined based on haemagglutination inhibition (HI) analysis using mAbs and the majority of viruses isolated in 2010 and 2011 fell into two of these clusters. An antigenic map based on polyclonal rat antisera showed that all virus isolates fell within one extended cluster. Accordingly, continuous surveillance and antigenic characterization will help us determine which virus isolate(s) should be used in poultry vaccine preparation. |
| doi_str_mv | 10.1099/vir.0.043299-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1551629408</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1551629408</sourcerecordid><originalsourceid>FETCH-LOGICAL-c464t-bfc2aa059e1ed4292da7262a0206bcda127c2e789792d469b4ccb6ab447b62c73</originalsourceid><addsrcrecordid>eNqFkb1v2zAQxYmiQeOkXTsWXApkkXs88UMcgyBNAgTpks4ERZ0MFjLtkpIL96-PErvNmOmAd797OLzH2GcBSwHWftvFvIQlyBqtreAdWwipVYXz6j1bACBWohbmlJ2V8gtASKnMB3aKaJU2tVqw1WUa44pSDLyLO8oljnvuU8dD3pRSZfJhjLtncdNzv4s-8Zj6YaL01_Nb9SD4_MFUqMwyv17ttyNvafxDlDgC6BcrBCE-spPeD4U-Hec5-_n9-vHqtrr_cXN3dXlfBanlWLV9QO9BWRLUSbTYeYMaPSDoNnReoAlIprFmXkltWxlCq30rpWk1BlOfs4uD7zZvfk9URreOJdAw-ESbqTihlNBoJTRvo8I02spa2hldHtCXVDL1bpvj2ue9E-Cee3BzCg7coQcH88GXo_fUrqn7j_8Lfga-HgFfgh_67FOI5ZXTWjcKm_oJibCPPQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1178694349</pqid></control><display><type>article</type><title>Antigenic diversity and cross-reactivity of avian influenza H5N1 viruses in Egypt between 2006 and 2011</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>EL-SHESHENY, Rabeh ; KAYALI, Ghazi ; KANDEIL, Ahmed ; ZHIPENG CAI ; BARAKAT, Ahmed B ; GHANIM, Hossam ; ALI, Mohamed A</creator><creatorcontrib>EL-SHESHENY, Rabeh ; KAYALI, Ghazi ; KANDEIL, Ahmed ; ZHIPENG CAI ; BARAKAT, Ahmed B ; GHANIM, Hossam ; ALI, Mohamed A</creatorcontrib><description>Influenza epidemics are a major health concern worldwide. Highly pathogenic avian influenza (HPAI) H5N1 viruses in Egypt have been subject to rapid genetic and antigenic changes since the first outbreak in February 2006 and have been endemic in poultry in Egypt since 2008. In this study, 33 H5N1 viruses isolated from avian hosts were antigenically analysed by using a panel of eight mAbs raised against the A/Viet Nam/1203/04 (H5N1; clade 1) and A/bar-headed goose/Qinghai-lake/1A/05 (H5N1; clade 2.2) influenza viruses. Rats were immunized with inactivated whole-virus vaccine produced by reverse genetics with the haemagglutinin and neuraminidase genes of eight antigenically different HPAI H5N1 virus isolates and six internal genes from A/Puerto Rico/8/1934 (PR8) to produce polyclonal antibodies. Cross-reactivity between the obtained polyclonal antibodies and the isolated viruses was assayed. Antigenic cartography of the isolated viruses showed that three antigenic clusters were defined based on haemagglutination inhibition (HI) analysis using mAbs and the majority of viruses isolated in 2010 and 2011 fell into two of these clusters. An antigenic map based on polyclonal rat antisera showed that all virus isolates fell within one extended cluster. Accordingly, continuous surveillance and antigenic characterization will help us determine which virus isolate(s) should be used in poultry vaccine preparation.</description><identifier>ISSN: 0022-1317</identifier><identifier>ISSN: 1465-2099</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.043299-0</identifier><identifier>PMID: 22956735</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; Antibodies, Viral ; Antigenic Variation ; Antigens, Viral - chemistry ; Antigens, Viral - genetics ; Biological and medical sciences ; Cross Reactions ; Egypt ; Fundamental and applied biological sciences. Psychology ; Hemagglutinin Glycoproteins, Influenza Virus - genetics ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; Influenza A Virus, H5N1 Subtype - classification ; Influenza A Virus, H5N1 Subtype - genetics ; Influenza A Virus, H5N1 Subtype - immunology ; Influenza A Virus, H5N1 Subtype - isolation & purification ; Influenza in Birds - virology ; Microbiology ; Miscellaneous ; Models, Molecular ; Molecular Sequence Data ; Phylogeny ; Poultry - virology ; Rats ; Sequence Homology, Amino Acid ; Virology</subject><ispartof>Journal of general virology, 2012-12, Vol.93 (Pt 12), p.2564-2574</ispartof><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-bfc2aa059e1ed4292da7262a0206bcda127c2e789792d469b4ccb6ab447b62c73</citedby><cites>FETCH-LOGICAL-c464t-bfc2aa059e1ed4292da7262a0206bcda127c2e789792d469b4ccb6ab447b62c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3745,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26668528$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22956735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EL-SHESHENY, Rabeh</creatorcontrib><creatorcontrib>KAYALI, Ghazi</creatorcontrib><creatorcontrib>KANDEIL, Ahmed</creatorcontrib><creatorcontrib>ZHIPENG CAI</creatorcontrib><creatorcontrib>BARAKAT, Ahmed B</creatorcontrib><creatorcontrib>GHANIM, Hossam</creatorcontrib><creatorcontrib>ALI, Mohamed A</creatorcontrib><title>Antigenic diversity and cross-reactivity of avian influenza H5N1 viruses in Egypt between 2006 and 2011</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Influenza epidemics are a major health concern worldwide. Highly pathogenic avian influenza (HPAI) H5N1 viruses in Egypt have been subject to rapid genetic and antigenic changes since the first outbreak in February 2006 and have been endemic in poultry in Egypt since 2008. In this study, 33 H5N1 viruses isolated from avian hosts were antigenically analysed by using a panel of eight mAbs raised against the A/Viet Nam/1203/04 (H5N1; clade 1) and A/bar-headed goose/Qinghai-lake/1A/05 (H5N1; clade 2.2) influenza viruses. Rats were immunized with inactivated whole-virus vaccine produced by reverse genetics with the haemagglutinin and neuraminidase genes of eight antigenically different HPAI H5N1 virus isolates and six internal genes from A/Puerto Rico/8/1934 (PR8) to produce polyclonal antibodies. Cross-reactivity between the obtained polyclonal antibodies and the isolated viruses was assayed. Antigenic cartography of the isolated viruses showed that three antigenic clusters were defined based on haemagglutination inhibition (HI) analysis using mAbs and the majority of viruses isolated in 2010 and 2011 fell into two of these clusters. An antigenic map based on polyclonal rat antisera showed that all virus isolates fell within one extended cluster. Accordingly, continuous surveillance and antigenic characterization will help us determine which virus isolate(s) should be used in poultry vaccine preparation.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibodies, Viral</subject><subject>Antigenic Variation</subject><subject>Antigens, Viral - chemistry</subject><subject>Antigens, Viral - genetics</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>Egypt</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - genetics</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - immunology</subject><subject>Influenza A Virus, H5N1 Subtype - classification</subject><subject>Influenza A Virus, H5N1 Subtype - genetics</subject><subject>Influenza A Virus, H5N1 Subtype - immunology</subject><subject>Influenza A Virus, H5N1 Subtype - isolation & purification</subject><subject>Influenza in Birds - virology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Poultry - virology</subject><subject>Rats</subject><subject>Sequence Homology, Amino Acid</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1v2zAQxYmiQeOkXTsWXApkkXs88UMcgyBNAgTpks4ERZ0MFjLtkpIL96-PErvNmOmAd797OLzH2GcBSwHWftvFvIQlyBqtreAdWwipVYXz6j1bACBWohbmlJ2V8gtASKnMB3aKaJU2tVqw1WUa44pSDLyLO8oljnvuU8dD3pRSZfJhjLtncdNzv4s-8Zj6YaL01_Nb9SD4_MFUqMwyv17ttyNvafxDlDgC6BcrBCE-spPeD4U-Hec5-_n9-vHqtrr_cXN3dXlfBanlWLV9QO9BWRLUSbTYeYMaPSDoNnReoAlIprFmXkltWxlCq30rpWk1BlOfs4uD7zZvfk9URreOJdAw-ESbqTihlNBoJTRvo8I02spa2hldHtCXVDL1bpvj2ue9E-Cee3BzCg7coQcH88GXo_fUrqn7j_8Lfga-HgFfgh_67FOI5ZXTWjcKm_oJibCPPQ</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>EL-SHESHENY, Rabeh</creator><creator>KAYALI, Ghazi</creator><creator>KANDEIL, Ahmed</creator><creator>ZHIPENG CAI</creator><creator>BARAKAT, Ahmed B</creator><creator>GHANIM, Hossam</creator><creator>ALI, Mohamed A</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T2</scope><scope>7U2</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20121201</creationdate><title>Antigenic diversity and cross-reactivity of avian influenza H5N1 viruses in Egypt between 2006 and 2011</title><author>EL-SHESHENY, Rabeh ; KAYALI, Ghazi ; KANDEIL, Ahmed ; ZHIPENG CAI ; BARAKAT, Ahmed B ; GHANIM, Hossam ; ALI, Mohamed A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-bfc2aa059e1ed4292da7262a0206bcda127c2e789792d469b4ccb6ab447b62c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibodies, Viral</topic><topic>Antigenic Variation</topic><topic>Antigens, Viral - chemistry</topic><topic>Antigens, Viral - genetics</topic><topic>Biological and medical sciences</topic><topic>Cross Reactions</topic><topic>Egypt</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - genetics</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - immunology</topic><topic>Influenza A Virus, H5N1 Subtype - classification</topic><topic>Influenza A Virus, H5N1 Subtype - genetics</topic><topic>Influenza A Virus, H5N1 Subtype - immunology</topic><topic>Influenza A Virus, H5N1 Subtype - isolation & purification</topic><topic>Influenza in Birds - virology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Poultry - virology</topic><topic>Rats</topic><topic>Sequence Homology, Amino Acid</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EL-SHESHENY, Rabeh</creatorcontrib><creatorcontrib>KAYALI, Ghazi</creatorcontrib><creatorcontrib>KANDEIL, Ahmed</creatorcontrib><creatorcontrib>ZHIPENG CAI</creatorcontrib><creatorcontrib>BARAKAT, Ahmed B</creatorcontrib><creatorcontrib>GHANIM, Hossam</creatorcontrib><creatorcontrib>ALI, Mohamed A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EL-SHESHENY, Rabeh</au><au>KAYALI, Ghazi</au><au>KANDEIL, Ahmed</au><au>ZHIPENG CAI</au><au>BARAKAT, Ahmed B</au><au>GHANIM, Hossam</au><au>ALI, Mohamed A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigenic diversity and cross-reactivity of avian influenza H5N1 viruses in Egypt between 2006 and 2011</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>93</volume><issue>Pt 12</issue><spage>2564</spage><epage>2574</epage><pages>2564-2574</pages><issn>0022-1317</issn><issn>1465-2099</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Influenza epidemics are a major health concern worldwide. Highly pathogenic avian influenza (HPAI) H5N1 viruses in Egypt have been subject to rapid genetic and antigenic changes since the first outbreak in February 2006 and have been endemic in poultry in Egypt since 2008. In this study, 33 H5N1 viruses isolated from avian hosts were antigenically analysed by using a panel of eight mAbs raised against the A/Viet Nam/1203/04 (H5N1; clade 1) and A/bar-headed goose/Qinghai-lake/1A/05 (H5N1; clade 2.2) influenza viruses. Rats were immunized with inactivated whole-virus vaccine produced by reverse genetics with the haemagglutinin and neuraminidase genes of eight antigenically different HPAI H5N1 virus isolates and six internal genes from A/Puerto Rico/8/1934 (PR8) to produce polyclonal antibodies. Cross-reactivity between the obtained polyclonal antibodies and the isolated viruses was assayed. Antigenic cartography of the isolated viruses showed that three antigenic clusters were defined based on haemagglutination inhibition (HI) analysis using mAbs and the majority of viruses isolated in 2010 and 2011 fell into two of these clusters. An antigenic map based on polyclonal rat antisera showed that all virus isolates fell within one extended cluster. Accordingly, continuous surveillance and antigenic characterization will help us determine which virus isolate(s) should be used in poultry vaccine preparation.</abstract><cop>Reading</cop><pub>Society for General Microbiology</pub><pmid>22956735</pmid><doi>10.1099/vir.0.043299-0</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 2012-12, Vol.93 (Pt 12), p.2564-2574 |
| issn | 0022-1317 1465-2099 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1551629408 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal Antibodies, Viral Antigenic Variation Antigens, Viral - chemistry Antigens, Viral - genetics Biological and medical sciences Cross Reactions Egypt Fundamental and applied biological sciences. Psychology Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - immunology Influenza A Virus, H5N1 Subtype - classification Influenza A Virus, H5N1 Subtype - genetics Influenza A Virus, H5N1 Subtype - immunology Influenza A Virus, H5N1 Subtype - isolation & purification Influenza in Birds - virology Microbiology Miscellaneous Models, Molecular Molecular Sequence Data Phylogeny Poultry - virology Rats Sequence Homology, Amino Acid Virology |
| title | Antigenic diversity and cross-reactivity of avian influenza H5N1 viruses in Egypt between 2006 and 2011 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T19%3A56%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antigenic%20diversity%20and%20cross-reactivity%20of%20avian%20influenza%20H5N1%20viruses%20in%20Egypt%20between%202006%20and%202011&rft.jtitle=Journal%20of%20general%20virology&rft.au=EL-SHESHENY,%20Rabeh&rft.date=2012-12-01&rft.volume=93&rft.issue=Pt%2012&rft.spage=2564&rft.epage=2574&rft.pages=2564-2574&rft.issn=0022-1317&rft.eissn=1465-2099&rft.coden=JGVIAY&rft_id=info:doi/10.1099/vir.0.043299-0&rft_dat=%3Cproquest_cross%3E1551629408%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1178694349&rft_id=info:pmid/22956735&rfr_iscdi=true |