Nanotopography-guided migration of T cells
T cells navigate a wide variety of tissues and organs for immune surveillance and effector functions. Although nanoscale topographical structures of extracellular matrices and stromal/endothelial cell surfaces in local tissues may guide the migration of T cells, there has been little opportunity to...
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Veröffentlicht in: | The Journal of immunology (1950) 2012-09, Vol.189 (5), p.2266-2273 |
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container_title | The Journal of immunology (1950) |
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creator | Kwon, Keon Woo Park, Hyoungjun Song, Kwang Hoon Choi, Jong-Cheol Ahn, Hyungmin Park, Moon Jeong Suh, Kahp-Yang Doh, Junsang |
description | T cells navigate a wide variety of tissues and organs for immune surveillance and effector functions. Although nanoscale topographical structures of extracellular matrices and stromal/endothelial cell surfaces in local tissues may guide the migration of T cells, there has been little opportunity to study how nanoscale topographical features affect T cell migration. In this study, we systematically investigated mechanisms of nanotopography-guided migration of T cells using nanoscale ridge/groove surfaces. The velocity and directionality of T cells on these nanostructured surfaces were quantitatively assessed with and without confinement, which is a key property of three-dimensional interstitial tissue spaces for leukocyte motility. Depending on the confinement, T cells exhibited different mechanisms for nanotopography-guided migration. Without confinement, actin polymerization-driven leading edge protrusion was guided toward the direction of nanogrooves via integrin-mediated adhesion. In contrast, T cells under confinement appeared to migrate along the direction of nanogrooves purely by mechanical effects, and integrin-mediated adhesion was dispensable. Therefore, surface nanotopography may play a prominent role in generating migratory patterns for T cells. Because the majority of cells in periphery migrate along the topography of extracellular matrices with much lower motility than T cells, nanotopography-guided migration of T cells would be an important strategy to efficiently perform cell-mediated immune responses by increasing chances of encountering other cells within a given amount of time. |
doi_str_mv | 10.4049/jimmunol.1102273 |
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Although nanoscale topographical structures of extracellular matrices and stromal/endothelial cell surfaces in local tissues may guide the migration of T cells, there has been little opportunity to study how nanoscale topographical features affect T cell migration. In this study, we systematically investigated mechanisms of nanotopography-guided migration of T cells using nanoscale ridge/groove surfaces. The velocity and directionality of T cells on these nanostructured surfaces were quantitatively assessed with and without confinement, which is a key property of three-dimensional interstitial tissue spaces for leukocyte motility. Depending on the confinement, T cells exhibited different mechanisms for nanotopography-guided migration. Without confinement, actin polymerization-driven leading edge protrusion was guided toward the direction of nanogrooves via integrin-mediated adhesion. In contrast, T cells under confinement appeared to migrate along the direction of nanogrooves purely by mechanical effects, and integrin-mediated adhesion was dispensable. Therefore, surface nanotopography may play a prominent role in generating migratory patterns for T cells. Because the majority of cells in periphery migrate along the topography of extracellular matrices with much lower motility than T cells, nanotopography-guided migration of T cells would be an important strategy to efficiently perform cell-mediated immune responses by increasing chances of encountering other cells within a given amount of time.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.1102273</identifier><identifier>PMID: 22844118</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Animals ; Cell Communication - immunology ; Cell Membrane - immunology ; Cell Membrane - ultrastructure ; Cell Movement - immunology ; Lymphocyte Activation - immunology ; Mice ; Mice, Transgenic ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Microscopy, Interference ; Molecular Sequence Data ; Nanotechnology - instrumentation ; Nanotechnology - methods ; Surface Properties ; T-Lymphocyte Subsets - cytology ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - ultrastructure</subject><ispartof>The Journal of immunology (1950), 2012-09, Vol.189 (5), p.2266-2273</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-aeb6c2b51f34bbe8abd45ae28e4decf8aeec4499c070762e71ecf1af06cc2bec3</citedby><cites>FETCH-LOGICAL-c374t-aeb6c2b51f34bbe8abd45ae28e4decf8aeec4499c070762e71ecf1af06cc2bec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22844118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kwon, Keon Woo</creatorcontrib><creatorcontrib>Park, Hyoungjun</creatorcontrib><creatorcontrib>Song, Kwang Hoon</creatorcontrib><creatorcontrib>Choi, Jong-Cheol</creatorcontrib><creatorcontrib>Ahn, Hyungmin</creatorcontrib><creatorcontrib>Park, Moon Jeong</creatorcontrib><creatorcontrib>Suh, Kahp-Yang</creatorcontrib><creatorcontrib>Doh, Junsang</creatorcontrib><title>Nanotopography-guided migration of T cells</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>T cells navigate a wide variety of tissues and organs for immune surveillance and effector functions. Although nanoscale topographical structures of extracellular matrices and stromal/endothelial cell surfaces in local tissues may guide the migration of T cells, there has been little opportunity to study how nanoscale topographical features affect T cell migration. In this study, we systematically investigated mechanisms of nanotopography-guided migration of T cells using nanoscale ridge/groove surfaces. The velocity and directionality of T cells on these nanostructured surfaces were quantitatively assessed with and without confinement, which is a key property of three-dimensional interstitial tissue spaces for leukocyte motility. Depending on the confinement, T cells exhibited different mechanisms for nanotopography-guided migration. Without confinement, actin polymerization-driven leading edge protrusion was guided toward the direction of nanogrooves via integrin-mediated adhesion. In contrast, T cells under confinement appeared to migrate along the direction of nanogrooves purely by mechanical effects, and integrin-mediated adhesion was dispensable. Therefore, surface nanotopography may play a prominent role in generating migratory patterns for T cells. Because the majority of cells in periphery migrate along the topography of extracellular matrices with much lower motility than T cells, nanotopography-guided migration of T cells would be an important strategy to efficiently perform cell-mediated immune responses by increasing chances of encountering other cells within a given amount of time.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cell Communication - immunology</subject><subject>Cell Membrane - immunology</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Movement - immunology</subject><subject>Lymphocyte Activation - immunology</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Fluorescence</subject><subject>Microscopy, Interference</subject><subject>Molecular Sequence Data</subject><subject>Nanotechnology - instrumentation</subject><subject>Nanotechnology - methods</subject><subject>Surface Properties</subject><subject>T-Lymphocyte Subsets - cytology</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - ultrastructure</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAQxS0EoqGwM6GMCCnl_BHbGVHFl1TBUubIcS4lVRKHOBn63-OqLSvT6U7vPd37EXJLYSFAZI_bum2nzjULSoExxc9IRNMUEilBnpMIwjGhSqoZufJ-CwASmLgkM8a0EJTqiDx8mM6NrnebwfTfu2Qz1SWWcVuHfaxdF7sqXscWm8Zfk4vKNB5vjnNOvl6e18u3ZPX5-r58WiWWKzEmBgtpWZHSiouiQG2KUqQGmUZRoq20QbRCZJkFBUoyVDRcqalA2mBDy-fk_pDbD-5nQj_mbe33H5gO3eTz0JDKUJan_0uBC5lqLWWQwkFqB-f9gFXeD3Vrhl0Q5XuY-QlmfoQZLHfH9KlosfwznOjxXxI3cis</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Kwon, Keon Woo</creator><creator>Park, Hyoungjun</creator><creator>Song, Kwang Hoon</creator><creator>Choi, Jong-Cheol</creator><creator>Ahn, Hyungmin</creator><creator>Park, Moon Jeong</creator><creator>Suh, Kahp-Yang</creator><creator>Doh, Junsang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20120901</creationdate><title>Nanotopography-guided migration of T cells</title><author>Kwon, Keon Woo ; Park, Hyoungjun ; Song, Kwang Hoon ; Choi, Jong-Cheol ; Ahn, Hyungmin ; Park, Moon Jeong ; Suh, Kahp-Yang ; Doh, Junsang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-aeb6c2b51f34bbe8abd45ae28e4decf8aeec4499c070762e71ecf1af06cc2bec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cell Communication - immunology</topic><topic>Cell Membrane - immunology</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell Movement - immunology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Electron, Transmission</topic><topic>Microscopy, Fluorescence</topic><topic>Microscopy, Interference</topic><topic>Molecular Sequence Data</topic><topic>Nanotechnology - instrumentation</topic><topic>Nanotechnology - methods</topic><topic>Surface Properties</topic><topic>T-Lymphocyte Subsets - cytology</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwon, Keon Woo</creatorcontrib><creatorcontrib>Park, Hyoungjun</creatorcontrib><creatorcontrib>Song, Kwang Hoon</creatorcontrib><creatorcontrib>Choi, Jong-Cheol</creatorcontrib><creatorcontrib>Ahn, Hyungmin</creatorcontrib><creatorcontrib>Park, Moon Jeong</creatorcontrib><creatorcontrib>Suh, Kahp-Yang</creatorcontrib><creatorcontrib>Doh, Junsang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwon, Keon Woo</au><au>Park, Hyoungjun</au><au>Song, Kwang Hoon</au><au>Choi, Jong-Cheol</au><au>Ahn, Hyungmin</au><au>Park, Moon Jeong</au><au>Suh, Kahp-Yang</au><au>Doh, Junsang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanotopography-guided migration of T cells</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>189</volume><issue>5</issue><spage>2266</spage><epage>2273</epage><pages>2266-2273</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>T cells navigate a wide variety of tissues and organs for immune surveillance and effector functions. Although nanoscale topographical structures of extracellular matrices and stromal/endothelial cell surfaces in local tissues may guide the migration of T cells, there has been little opportunity to study how nanoscale topographical features affect T cell migration. In this study, we systematically investigated mechanisms of nanotopography-guided migration of T cells using nanoscale ridge/groove surfaces. The velocity and directionality of T cells on these nanostructured surfaces were quantitatively assessed with and without confinement, which is a key property of three-dimensional interstitial tissue spaces for leukocyte motility. Depending on the confinement, T cells exhibited different mechanisms for nanotopography-guided migration. Without confinement, actin polymerization-driven leading edge protrusion was guided toward the direction of nanogrooves via integrin-mediated adhesion. In contrast, T cells under confinement appeared to migrate along the direction of nanogrooves purely by mechanical effects, and integrin-mediated adhesion was dispensable. Therefore, surface nanotopography may play a prominent role in generating migratory patterns for T cells. Because the majority of cells in periphery migrate along the topography of extracellular matrices with much lower motility than T cells, nanotopography-guided migration of T cells would be an important strategy to efficiently perform cell-mediated immune responses by increasing chances of encountering other cells within a given amount of time.</abstract><cop>United States</cop><pmid>22844118</pmid><doi>10.4049/jimmunol.1102273</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acid Sequence Animals Cell Communication - immunology Cell Membrane - immunology Cell Membrane - ultrastructure Cell Movement - immunology Lymphocyte Activation - immunology Mice Mice, Transgenic Microscopy, Electron, Scanning Microscopy, Electron, Transmission Microscopy, Fluorescence Microscopy, Interference Molecular Sequence Data Nanotechnology - instrumentation Nanotechnology - methods Surface Properties T-Lymphocyte Subsets - cytology T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - ultrastructure |
title | Nanotopography-guided migration of T cells |
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