Thalassolituus marinus sp. nov., a hydrocarbon-utilizing marine bacterium

Gram-negative strains, motile by a single polar flagellum, non-pigmented and with a curved rod-shaped morphology, designated IMCC1826T and IMCC1883, were isolated from a surface seawater sample from the Yellow Sea. The two strains shared 99.9 % 16S rRNA gene sequence similarity and showed 92 % DNA–D...

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Veröffentlicht in:International journal of systematic and evolutionary microbiology 2013-06, Vol.63 (Pt 6), p.2234-2238
Hauptverfasser: Choi, Ahyoung, Cho, Jang-Cheon
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description Gram-negative strains, motile by a single polar flagellum, non-pigmented and with a curved rod-shaped morphology, designated IMCC1826T and IMCC1883, were isolated from a surface seawater sample from the Yellow Sea. The two strains shared 99.9 % 16S rRNA gene sequence similarity and showed 92 % DNA–DNA relatedness, suggesting that they belonged to the same genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates were related most closely to the type strain of Thalassolituus oleivorans with a sequence similarity of 96.4 % and formed a robust phyletic lineage with T. oleivorans. DNA–DNA relatedness between the two strains and T. oleivorans DSM 14913T was 8.7–11.6 %. A putative alkane hydroxylase (alkB) gene was detected in strain IMCC1826T by PCR, but the amino acid sequence of the gene was distantly related to that of the AlkB homologue of T. oleivorans DSM 14913T. As expected from the presence of the alkB gene, the new strains utilized n-tetradecane and n-hexadecane as a carbon source. The DNA G+C content was 54.6–56.0 mol% and the main isoprenoid quinone detected was Q-9. Polar lipids of strain IMCC1826T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and amino-group-containing lipids. On the basis of taxonomic data obtained in this study, strains IMCC1826T and IMCC1883 represent a novel species of the genus Thalassolituus, for which the name Thalassolituus marinus sp. nov. is proposed, with IMCC1826T ( = KCTC 23084T = NBRC 107590T) as the type strain.
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The two strains shared 99.9 % 16S rRNA gene sequence similarity and showed 92 % DNA–DNA relatedness, suggesting that they belonged to the same genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates were related most closely to the type strain of Thalassolituus oleivorans with a sequence similarity of 96.4 % and formed a robust phyletic lineage with T. oleivorans. DNA–DNA relatedness between the two strains and T. oleivorans DSM 14913T was 8.7–11.6 %. A putative alkane hydroxylase (alkB) gene was detected in strain IMCC1826T by PCR, but the amino acid sequence of the gene was distantly related to that of the AlkB homologue of T. oleivorans DSM 14913T. As expected from the presence of the alkB gene, the new strains utilized n-tetradecane and n-hexadecane as a carbon source. The DNA G+C content was 54.6–56.0 mol% and the main isoprenoid quinone detected was Q-9. 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Polar lipids of strain IMCC1826T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and amino-group-containing lipids. On the basis of taxonomic data obtained in this study, strains IMCC1826T and IMCC1883 represent a novel species of the genus Thalassolituus, for which the name Thalassolituus marinus sp. nov. is proposed, with IMCC1826T ( = KCTC 23084T = NBRC 107590T) as the type strain.</description><subject>amino acid sequences</subject><subject>Bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>carbon</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - analysis</subject><subject>flagellum</subject><subject>genes</subject><subject>hexadecane</subject><subject>Hydrocarbons - metabolism</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Oceanospirillaceae - classification</subject><subject>Oceanospirillaceae - genetics</subject><subject>Oceanospirillaceae - isolation &amp; purification</subject><subject>Oceanospirillaceae - metabolism</subject><subject>phosphatidylethanolamines</subject><subject>Phylogeny</subject><subject>polymerase chain reaction</subject><subject>Republic of Korea</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>seawater</subject><subject>Seawater - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>sequence homology</subject><subject>Thalassolituus</subject><subject>Vitamin K 2 - analogs &amp; derivatives</subject><subject>Vitamin K 2 - analysis</subject><subject>Water Microbiology</subject><issn>1466-5034</issn><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1PwzAQhi0EoqWwMkJGBhLOdmzHI6r4qFSJgXa2HMdpXaVJsROk8utJlYLYmO50eu6V7jmErjEkGKR8cJuQQAIppxmN4QSNccp5zICmp3_6EboIYQPQDwDO0YhQnGYYyBjNFmtd6RCayrVdF6Kt9q7ua9glUd18JveRjtb7wjdG-7yp4651lfty9WogbZRr01rvuu0lOit1FezVsU7Q8vlpMX2N528vs-njPDYkI22MhZQGiiyzkjCmuTBgQZCUYW0kFVpiLHHOWVEA10UKhpW2xGCEkIwZXdIJuhtyd7756Gxo1dYFY6tK17bpgsKMYU54BvR_lHLOhMACejQZUOObELwt1c67_sS9wqAOplVvWoEaTKvDws0xu8u3tvjFf9T2wO0AlLpReuVdUMt3Apgd3kClJPQbVaWB5g</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Choi, Ahyoung</creator><creator>Cho, Jang-Cheon</creator><general>International Union of Microbiological Societies</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7TN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20130601</creationdate><title>Thalassolituus marinus sp. nov., a hydrocarbon-utilizing marine bacterium</title><author>Choi, Ahyoung ; 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derivatives</topic><topic>Vitamin K 2 - analysis</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Ahyoung</creatorcontrib><creatorcontrib>Cho, Jang-Cheon</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; 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The two strains shared 99.9 % 16S rRNA gene sequence similarity and showed 92 % DNA–DNA relatedness, suggesting that they belonged to the same genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates were related most closely to the type strain of Thalassolituus oleivorans with a sequence similarity of 96.4 % and formed a robust phyletic lineage with T. oleivorans. DNA–DNA relatedness between the two strains and T. oleivorans DSM 14913T was 8.7–11.6 %. A putative alkane hydroxylase (alkB) gene was detected in strain IMCC1826T by PCR, but the amino acid sequence of the gene was distantly related to that of the AlkB homologue of T. oleivorans DSM 14913T. As expected from the presence of the alkB gene, the new strains utilized n-tetradecane and n-hexadecane as a carbon source. The DNA G+C content was 54.6–56.0 mol% and the main isoprenoid quinone detected was Q-9. Polar lipids of strain IMCC1826T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and amino-group-containing lipids. On the basis of taxonomic data obtained in this study, strains IMCC1826T and IMCC1883 represent a novel species of the genus Thalassolituus, for which the name Thalassolituus marinus sp. nov. is proposed, with IMCC1826T ( = KCTC 23084T = NBRC 107590T) as the type strain.</abstract><cop>England</cop><pub>International Union of Microbiological Societies</pub><pmid>23148102</pmid><doi>10.1099/ijs.0.046383-0</doi><tpages>5</tpages></addata></record>
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subjects amino acid sequences
Bacteria
Bacterial Typing Techniques
Base Composition
carbon
DNA
DNA, Bacterial - genetics
Fatty Acids - analysis
flagellum
genes
hexadecane
Hydrocarbons - metabolism
Molecular Sequence Data
nucleotide sequences
Oceanospirillaceae - classification
Oceanospirillaceae - genetics
Oceanospirillaceae - isolation & purification
Oceanospirillaceae - metabolism
phosphatidylethanolamines
Phylogeny
polymerase chain reaction
Republic of Korea
ribosomal RNA
RNA, Ribosomal, 16S - genetics
seawater
Seawater - microbiology
Sequence Analysis, DNA
sequence homology
Thalassolituus
Vitamin K 2 - analogs & derivatives
Vitamin K 2 - analysis
Water Microbiology
title Thalassolituus marinus sp. nov., a hydrocarbon-utilizing marine bacterium
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