Polyphenol-rich extract from Pinus sylvestris L. bark--chemical and antitumor studies
Pinus sylvestris L. is a very important timber tree in Romania, the bark being the main waste from the wood processing. The aim of this study was to investigate the chemical composition and antitumor effects against HeLa cells of a polyphenol-rich extract from Pinus sylvestris L. bark. The polypheno...
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| Veröffentlicht in: | Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi 2014-04, Vol.118 (2), p.551-557 |
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| container_title | Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi |
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| creator | Amalinei, Roxana Laura Mihailescu Trifan, Adriana Cioanca, Oana Miron, S D Mihai, C T Rotinberg, P Miron, Anca |
| description | Pinus sylvestris L. is a very important timber tree in Romania, the bark being the main waste from the wood processing.
The aim of this study was to investigate the chemical composition and antitumor effects against HeLa cells of a polyphenol-rich extract from Pinus sylvestris L. bark.
The polyphenolic content and profile were analyzed by means of spectrophotometry and RP-HPLC-UV/ESI-MS in the negative ion mode. The antitumor activity was investigated using two in vitro assays: MTT and flow cytometric apoptosis assays.
Pine bark extract contained a high total phenolic content (48.16 +/- 0.29%). RP-HPLC-UV/ESI-MS analysis allowed the identification of taxifolin, a taxifolin-hexoside and several procyanidins (two monomers, three dimers and three trimers). At 200 microg/mL, pine bark extract exhibited a high cytotoxicity against HeLa cells (82.10 +/- 1.95%). Flow cytometry revealed the ability of pine bark extract to induce apoptosis: 71.97 +/- 0.96% apoptotic cells were determined in HeLa cells after a 48 h incubation with pine bark extract (200 microg/mL).
The ability of pine bark extract to reduce viability and induce apoptosis in HeLa cells suggests the presence of compounds with antitumor properties and encourages further studies for their isolation and characterization. |
| format | Article |
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The aim of this study was to investigate the chemical composition and antitumor effects against HeLa cells of a polyphenol-rich extract from Pinus sylvestris L. bark.
The polyphenolic content and profile were analyzed by means of spectrophotometry and RP-HPLC-UV/ESI-MS in the negative ion mode. The antitumor activity was investigated using two in vitro assays: MTT and flow cytometric apoptosis assays.
Pine bark extract contained a high total phenolic content (48.16 +/- 0.29%). RP-HPLC-UV/ESI-MS analysis allowed the identification of taxifolin, a taxifolin-hexoside and several procyanidins (two monomers, three dimers and three trimers). At 200 microg/mL, pine bark extract exhibited a high cytotoxicity against HeLa cells (82.10 +/- 1.95%). Flow cytometry revealed the ability of pine bark extract to induce apoptosis: 71.97 +/- 0.96% apoptotic cells were determined in HeLa cells after a 48 h incubation with pine bark extract (200 microg/mL).
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The aim of this study was to investigate the chemical composition and antitumor effects against HeLa cells of a polyphenol-rich extract from Pinus sylvestris L. bark.
The polyphenolic content and profile were analyzed by means of spectrophotometry and RP-HPLC-UV/ESI-MS in the negative ion mode. The antitumor activity was investigated using two in vitro assays: MTT and flow cytometric apoptosis assays.
Pine bark extract contained a high total phenolic content (48.16 +/- 0.29%). RP-HPLC-UV/ESI-MS analysis allowed the identification of taxifolin, a taxifolin-hexoside and several procyanidins (two monomers, three dimers and three trimers). At 200 microg/mL, pine bark extract exhibited a high cytotoxicity against HeLa cells (82.10 +/- 1.95%). Flow cytometry revealed the ability of pine bark extract to induce apoptosis: 71.97 +/- 0.96% apoptotic cells were determined in HeLa cells after a 48 h incubation with pine bark extract (200 microg/mL).
The ability of pine bark extract to reduce viability and induce apoptosis in HeLa cells suggests the presence of compounds with antitumor properties and encourages further studies for their isolation and characterization.</description><subject>Anti-Inflammatory Agents, Non-Steroidal - chemistry</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Chromatography, Reverse-Phase</subject><subject>HeLa Cells - drug effects</subject><subject>Humans</subject><subject>Phytotherapy - methods</subject><subject>Pinus sylvestris</subject><subject>Plant Bark</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Polyphenols - analysis</subject><subject>Polyphenols - chemistry</subject><subject>Polyphenols - pharmacology</subject><subject>Proanthocyanidins - chemistry</subject><subject>Proanthocyanidins - pharmacology</subject><subject>Quercetin - analogs & derivatives</subject><subject>Quercetin - chemistry</subject><subject>Quercetin - pharmacology</subject><subject>Romania</subject><subject>Spectrophotometry</subject><issn>0048-7848</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtKxDAARbNQnGGcX5As3UTyaNJ0KYMvGHBAXZc0SWkwbWoeYv_eguPicDeHy-VegC3GlUS1rOQG7FNyHcaC15Xk_ApsKMe1qBnego9T8Ms82Cl4FJ0eoP3JUekM-xhGeHJTSTAt_tumHF2CxzvYqfiJkB7s6LTyUE1mJbtcxhBhysU4m67BZa98svtz7sDb48P74RkdX59eDvdHNFNCMuqsqI2SjPVCkhWuNWmwNEI3uJe6MbpjPVVCa9pJpqgVVAohmOFGUcl24PavdY7hq6wL29Elbb1Xkw0ltYRzgimWVbOqN2e1dKM17RzdqOLS_h_BfgFCQVqk</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Amalinei, Roxana Laura Mihailescu</creator><creator>Trifan, Adriana</creator><creator>Cioanca, Oana</creator><creator>Miron, S D</creator><creator>Mihai, C T</creator><creator>Rotinberg, P</creator><creator>Miron, Anca</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Polyphenol-rich extract from Pinus sylvestris L. bark--chemical and antitumor studies</title><author>Amalinei, Roxana Laura Mihailescu ; Trifan, Adriana ; Cioanca, Oana ; Miron, S D ; Mihai, C T ; Rotinberg, P ; Miron, Anca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-be67da833f681f685cc1908d6c90f8c9dcb3f2a6cc2b83a2e6286663d5da283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anti-Inflammatory Agents, Non-Steroidal - chemistry</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Chromatography, Reverse-Phase</topic><topic>HeLa Cells - drug effects</topic><topic>Humans</topic><topic>Phytotherapy - methods</topic><topic>Pinus sylvestris</topic><topic>Plant Bark</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Polyphenols - analysis</topic><topic>Polyphenols - chemistry</topic><topic>Polyphenols - pharmacology</topic><topic>Proanthocyanidins - chemistry</topic><topic>Proanthocyanidins - pharmacology</topic><topic>Quercetin - analogs & derivatives</topic><topic>Quercetin - chemistry</topic><topic>Quercetin - pharmacology</topic><topic>Romania</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amalinei, Roxana Laura Mihailescu</creatorcontrib><creatorcontrib>Trifan, Adriana</creatorcontrib><creatorcontrib>Cioanca, Oana</creatorcontrib><creatorcontrib>Miron, S D</creatorcontrib><creatorcontrib>Mihai, C T</creatorcontrib><creatorcontrib>Rotinberg, P</creatorcontrib><creatorcontrib>Miron, Anca</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amalinei, Roxana Laura Mihailescu</au><au>Trifan, Adriana</au><au>Cioanca, Oana</au><au>Miron, S D</au><au>Mihai, C T</au><au>Rotinberg, P</au><au>Miron, Anca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyphenol-rich extract from Pinus sylvestris L. bark--chemical and antitumor studies</atitle><jtitle>Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi</jtitle><addtitle>Rev Med Chir Soc Med Nat Iasi</addtitle><date>2014-04</date><risdate>2014</risdate><volume>118</volume><issue>2</issue><spage>551</spage><epage>557</epage><pages>551-557</pages><issn>0048-7848</issn><abstract>Pinus sylvestris L. is a very important timber tree in Romania, the bark being the main waste from the wood processing.
The aim of this study was to investigate the chemical composition and antitumor effects against HeLa cells of a polyphenol-rich extract from Pinus sylvestris L. bark.
The polyphenolic content and profile were analyzed by means of spectrophotometry and RP-HPLC-UV/ESI-MS in the negative ion mode. The antitumor activity was investigated using two in vitro assays: MTT and flow cytometric apoptosis assays.
Pine bark extract contained a high total phenolic content (48.16 +/- 0.29%). RP-HPLC-UV/ESI-MS analysis allowed the identification of taxifolin, a taxifolin-hexoside and several procyanidins (two monomers, three dimers and three trimers). At 200 microg/mL, pine bark extract exhibited a high cytotoxicity against HeLa cells (82.10 +/- 1.95%). Flow cytometry revealed the ability of pine bark extract to induce apoptosis: 71.97 +/- 0.96% apoptotic cells were determined in HeLa cells after a 48 h incubation with pine bark extract (200 microg/mL).
The ability of pine bark extract to reduce viability and induce apoptosis in HeLa cells suggests the presence of compounds with antitumor properties and encourages further studies for their isolation and characterization.</abstract><cop>Romania</cop><pmid>25076730</pmid><tpages>7</tpages></addata></record> |
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| subjects | Anti-Inflammatory Agents, Non-Steroidal - chemistry Anti-Inflammatory Agents, Non-Steroidal - pharmacology Apoptosis - drug effects Chromatography, Reverse-Phase HeLa Cells - drug effects Humans Phytotherapy - methods Pinus sylvestris Plant Bark Plant Extracts - analysis Plant Extracts - chemistry Plant Extracts - pharmacology Polyphenols - analysis Polyphenols - chemistry Polyphenols - pharmacology Proanthocyanidins - chemistry Proanthocyanidins - pharmacology Quercetin - analogs & derivatives Quercetin - chemistry Quercetin - pharmacology Romania Spectrophotometry |
| title | Polyphenol-rich extract from Pinus sylvestris L. bark--chemical and antitumor studies |
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