Supercoiled DNA Repair in Thymocyte Fractions Differing in Radiosensitivity
Summary Mouse thymocyte fractions were isolated on the basis of buoyant density in a step-gradient of human serum albumin. These fractions were characterized by cell size, radiosensitivity and hydrocortisone sensitivity, and by labelled precursor incorporation into DNA, RNA and proteins. The relativ...
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| Veröffentlicht in: | International journal of radiation biology 1982-01, Vol.42 (1), p.31-44 |
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| container_title | International journal of radiation biology |
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| creator | Filippovich, I.V. Sorokina, N.I. Soldatenkov, V.A. Romantzev, E.F. |
| description | Summary
Mouse thymocyte fractions were isolated on the basis of buoyant density in a step-gradient of human serum albumin. These fractions were characterized by cell size, radiosensitivity and hydrocortisone sensitivity, and by labelled precursor incorporation into DNA, RNA and proteins. The relative sedimentation of nucleoids in sucrose gradient was also determined. Large and more radioresistant thymocytes were characterized by an increased rate of DNA repair compared with the fraction of radiosensitive small lymphocytes. Nucleoids prepared from the small thymocyte fraction had greater relative sedimentation rates than those derived from large cells. The response of relative sedimentation of nucleoids from large and small thymocyte fractions to ethidium bromide concentration does not permit a conclusion on different superhelix density for DNA in cells of these fractions. At the same time the estimate of the supercoiled domain size for nucleoids of large and small thymocyte fractions showed that in the more radiosensitive small cells domains of greater size were predominant. |
| doi_str_mv | 10.1080/09553008214550891 |
| format | Article |
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Mouse thymocyte fractions were isolated on the basis of buoyant density in a step-gradient of human serum albumin. These fractions were characterized by cell size, radiosensitivity and hydrocortisone sensitivity, and by labelled precursor incorporation into DNA, RNA and proteins. The relative sedimentation of nucleoids in sucrose gradient was also determined. Large and more radioresistant thymocytes were characterized by an increased rate of DNA repair compared with the fraction of radiosensitive small lymphocytes. Nucleoids prepared from the small thymocyte fraction had greater relative sedimentation rates than those derived from large cells. The response of relative sedimentation of nucleoids from large and small thymocyte fractions to ethidium bromide concentration does not permit a conclusion on different superhelix density for DNA in cells of these fractions. At the same time the estimate of the supercoiled domain size for nucleoids of large and small thymocyte fractions showed that in the more radiosensitive small cells domains of greater size were predominant.</description><identifier>ISSN: 0955-3002</identifier><identifier>ISSN: 0020-7616</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553008214550891</identifier><identifier>PMID: 6980853</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Cell Survival - radiation effects ; Cobalt Radioisotopes ; DNA Repair ; DNA, Superhelical - radiation effects ; Dose-Response Relationship, Radiation ; Gamma Rays ; In Vitro Techniques ; Male ; Mice ; T-Lymphocytes - radiation effects ; Time Factors</subject><ispartof>International journal of radiation biology, 1982-01, Vol.42 (1), p.31-44</ispartof><rights>1982 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-fd1d47b46b1db68d6ebb2bd8dc04c1bf9eb7bada07dc7e09054e9ad2e7c768f83</citedby><cites>FETCH-LOGICAL-c432t-fd1d47b46b1db68d6ebb2bd8dc04c1bf9eb7bada07dc7e09054e9ad2e7c768f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553008214550891$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553008214550891$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6980853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filippovich, I.V.</creatorcontrib><creatorcontrib>Sorokina, N.I.</creatorcontrib><creatorcontrib>Soldatenkov, V.A.</creatorcontrib><creatorcontrib>Romantzev, E.F.</creatorcontrib><title>Supercoiled DNA Repair in Thymocyte Fractions Differing in Radiosensitivity</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><description>Summary
Mouse thymocyte fractions were isolated on the basis of buoyant density in a step-gradient of human serum albumin. These fractions were characterized by cell size, radiosensitivity and hydrocortisone sensitivity, and by labelled precursor incorporation into DNA, RNA and proteins. The relative sedimentation of nucleoids in sucrose gradient was also determined. Large and more radioresistant thymocytes were characterized by an increased rate of DNA repair compared with the fraction of radiosensitive small lymphocytes. Nucleoids prepared from the small thymocyte fraction had greater relative sedimentation rates than those derived from large cells. The response of relative sedimentation of nucleoids from large and small thymocyte fractions to ethidium bromide concentration does not permit a conclusion on different superhelix density for DNA in cells of these fractions. At the same time the estimate of the supercoiled domain size for nucleoids of large and small thymocyte fractions showed that in the more radiosensitive small cells domains of greater size were predominant.</description><subject>Animals</subject><subject>Cell Survival - radiation effects</subject><subject>Cobalt Radioisotopes</subject><subject>DNA Repair</subject><subject>DNA, Superhelical - radiation effects</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Gamma Rays</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mice</subject><subject>T-Lymphocytes - radiation effects</subject><subject>Time Factors</subject><issn>0955-3002</issn><issn>0020-7616</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLw0AUhQdRaq3-ABdCVu6id_Kc4KpYq2JRqHUd5nHHTkkydSYR8u9NaXEjuLoHvnMO3EPIJYUbCgxuoUjTGIBFNElTYAU9ImMaZ1EYD-SYjHd80BCdkjPvNzAoiNmIjLKCAUvjMXl577bopDUVqmD2Og2WuOXGBaYJVuu-trJvMZg7LltjGx_MjNboTPO5Myy5MtZj401rvk3bn5MTzSuPF4c7IR_zh9X9U7h4e3y-ny5CmcRRG2pFVZKLJBNUiYypDIWIhGJKQiKp0AWKXHDFIVcyRyggTbDgKsJc5hnTLJ6Q633v1tmvDn1b1sZLrCreoO18SdOkYHkWDUa6N0pnvXeoy60zNXd9SaHcDVj-GXDIXB3KO1Gj-k0cFhv43Z6bRltX8zXyql1L7rDc2M41w-P_tP8Adft-6A</recordid><startdate>19820101</startdate><enddate>19820101</enddate><creator>Filippovich, I.V.</creator><creator>Sorokina, N.I.</creator><creator>Soldatenkov, V.A.</creator><creator>Romantzev, E.F.</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19820101</creationdate><title>Supercoiled DNA Repair in Thymocyte Fractions Differing in Radiosensitivity</title><author>Filippovich, I.V. ; Sorokina, N.I. ; Soldatenkov, V.A. ; Romantzev, E.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-fd1d47b46b1db68d6ebb2bd8dc04c1bf9eb7bada07dc7e09054e9ad2e7c768f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Cell Survival - radiation effects</topic><topic>Cobalt Radioisotopes</topic><topic>DNA Repair</topic><topic>DNA, Superhelical - radiation effects</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Gamma Rays</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mice</topic><topic>T-Lymphocytes - radiation effects</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filippovich, I.V.</creatorcontrib><creatorcontrib>Sorokina, N.I.</creatorcontrib><creatorcontrib>Soldatenkov, V.A.</creatorcontrib><creatorcontrib>Romantzev, E.F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filippovich, I.V.</au><au>Sorokina, N.I.</au><au>Soldatenkov, V.A.</au><au>Romantzev, E.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supercoiled DNA Repair in Thymocyte Fractions Differing in Radiosensitivity</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol Relat Stud Phys Chem Med</addtitle><date>1982-01-01</date><risdate>1982</risdate><volume>42</volume><issue>1</issue><spage>31</spage><epage>44</epage><pages>31-44</pages><issn>0955-3002</issn><issn>0020-7616</issn><eissn>1362-3095</eissn><abstract>Summary
Mouse thymocyte fractions were isolated on the basis of buoyant density in a step-gradient of human serum albumin. These fractions were characterized by cell size, radiosensitivity and hydrocortisone sensitivity, and by labelled precursor incorporation into DNA, RNA and proteins. The relative sedimentation of nucleoids in sucrose gradient was also determined. Large and more radioresistant thymocytes were characterized by an increased rate of DNA repair compared with the fraction of radiosensitive small lymphocytes. Nucleoids prepared from the small thymocyte fraction had greater relative sedimentation rates than those derived from large cells. The response of relative sedimentation of nucleoids from large and small thymocyte fractions to ethidium bromide concentration does not permit a conclusion on different superhelix density for DNA in cells of these fractions. At the same time the estimate of the supercoiled domain size for nucleoids of large and small thymocyte fractions showed that in the more radiosensitive small cells domains of greater size were predominant.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>6980853</pmid><doi>10.1080/09553008214550891</doi><tpages>14</tpages></addata></record> |
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| ispartof | International journal of radiation biology, 1982-01, Vol.42 (1), p.31-44 |
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| source | Taylor & Francis:Master (3349 titles); MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | Animals Cell Survival - radiation effects Cobalt Radioisotopes DNA Repair DNA, Superhelical - radiation effects Dose-Response Relationship, Radiation Gamma Rays In Vitro Techniques Male Mice T-Lymphocytes - radiation effects Time Factors |
| title | Supercoiled DNA Repair in Thymocyte Fractions Differing in Radiosensitivity |
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