Effects of Deferoxamine on the Repair Ability of Dental Pulp Cells In Vitro
Abstract Introduction In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia...
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| Veröffentlicht in: | Journal of endodontics 2014-08, Vol.40 (8), p.1100-1104 |
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| creator | Jiang, Long, DDS, PhD Peng, Wei-Wei, DDS, MDS Li, Li-Fen, DDS, PhD Du, Rong, DDS, PhD Wu, Tian-Tian, DDS, MDS Zhou, Zhuo-Jun, DDS, MDS Zhao, Jun-Jun, DDS, PhD Yang, Ya, DDS, PhD Qu, Dong-Lin, BDS Zhu, Ya-Qin, DDS, PhD |
| description | Abstract Introduction In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs. Methods DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting. Results Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO. Conclusions These results suggest that DFO might improve the repair ability of DPCs by HIF-1α. |
| doi_str_mv | 10.1016/j.joen.2013.12.016 |
| format | Article |
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However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs. Methods DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting. Results Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO. Conclusions These results suggest that DFO might improve the repair ability of DPCs by HIF-1α.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2013.12.016</identifier><identifier>PMID: 25069915</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Alkaline Phosphatase - analysis ; Calcification, Physiologic - drug effects ; Cell Culture Techniques ; Cell Differentiation - drug effects ; Cell Hypoxia - drug effects ; Cell Movement - drug effects ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Coloring Agents ; Deferoxamine ; Deferoxamine - pharmacology ; Dental Pulp - cytology ; Dental Pulp - drug effects ; dental pulp cells ; Dentistry ; Endocrinology & Metabolism ; Fluorescent Dyes ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit - analysis ; hypoxia-inducible factors-1α ; Odontoblasts - drug effects ; Osteocalcin - analysis ; Regeneration - drug effects ; repair ability ; Siderophores - pharmacology ; Tetrazolium Salts ; Thiazoles ; Young Adult</subject><ispartof>Journal of endodontics, 2014-08, Vol.40 (8), p.1100-1104</ispartof><rights>American Association of Endodontists</rights><rights>2014 American Association of Endodontists</rights><rights>Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-c294d845d7c7f612954bb102e484cda2e3c0e55b76155962f1df5cb1212da9e03</citedby><cites>FETCH-LOGICAL-c547t-c294d845d7c7f612954bb102e484cda2e3c0e55b76155962f1df5cb1212da9e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.joen.2013.12.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25069915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Long, DDS, PhD</creatorcontrib><creatorcontrib>Peng, Wei-Wei, DDS, MDS</creatorcontrib><creatorcontrib>Li, Li-Fen, DDS, PhD</creatorcontrib><creatorcontrib>Du, Rong, DDS, PhD</creatorcontrib><creatorcontrib>Wu, Tian-Tian, DDS, MDS</creatorcontrib><creatorcontrib>Zhou, Zhuo-Jun, DDS, MDS</creatorcontrib><creatorcontrib>Zhao, Jun-Jun, DDS, PhD</creatorcontrib><creatorcontrib>Yang, Ya, DDS, PhD</creatorcontrib><creatorcontrib>Qu, Dong-Lin, BDS</creatorcontrib><creatorcontrib>Zhu, Ya-Qin, DDS, PhD</creatorcontrib><title>Effects of Deferoxamine on the Repair Ability of Dental Pulp Cells In Vitro</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract Introduction In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs. Methods DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting. Results Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO. Conclusions These results suggest that DFO might improve the repair ability of DPCs by HIF-1α.</description><subject>Adolescent</subject><subject>Alkaline Phosphatase - analysis</subject><subject>Calcification, Physiologic - drug effects</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Hypoxia - drug effects</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Coloring Agents</subject><subject>Deferoxamine</subject><subject>Deferoxamine - pharmacology</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>dental pulp cells</subject><subject>Dentistry</subject><subject>Endocrinology & Metabolism</subject><subject>Fluorescent Dyes</subject><subject>Humans</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - analysis</subject><subject>hypoxia-inducible factors-1α</subject><subject>Odontoblasts - drug effects</subject><subject>Osteocalcin - analysis</subject><subject>Regeneration - drug effects</subject><subject>repair ability</subject><subject>Siderophores - pharmacology</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Young Adult</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEUhoMoTjv6Ai4kSzdV5uRS1QERhnbUgQbF2zakUieYsrrSJlViv43P4pOZokcXLlwFDt__c_IdQh4Dq4FB82yoh4hTzRmIGnhdRnfIBrbtthJKybtkw5jWFRdaX5AHOQ-MQStEe59ccMUarUFtyP7ae3RzptHTl-gxxR_2ECakcaLzF6Tv8WhDolddGMN8OlPTbEf6bhmPdIfjmOnN9Ovn5zCn-JDc83bM-Oj2vSSfXl1_3L2p9m9f3-yu9pVTsp0rx7Xst1L1rWt9A1wr2XXAOMqtdL3lKBxDpbq2AaV0wz30XrkOOPDeamTikjw99x5T_LZgns0hZFd2sRPGJRtQUjcCGtYWlJ9Rl2LOCb05pnCw6WSAmdWiGcxq0awWDXBTRiX05LZ_6Q7Y_4380VaA52cAyy-_B0wmu4CTwz6kYtP0Mfy__8U_cTeGKTg7fsUT5iEuaSr-DJhcAubDesf1jCAYgBSN-A1dupdK</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Jiang, Long, DDS, PhD</creator><creator>Peng, Wei-Wei, DDS, MDS</creator><creator>Li, Li-Fen, DDS, PhD</creator><creator>Du, Rong, DDS, PhD</creator><creator>Wu, Tian-Tian, DDS, MDS</creator><creator>Zhou, Zhuo-Jun, DDS, MDS</creator><creator>Zhao, Jun-Jun, DDS, PhD</creator><creator>Yang, Ya, DDS, PhD</creator><creator>Qu, Dong-Lin, BDS</creator><creator>Zhu, Ya-Qin, DDS, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140801</creationdate><title>Effects of Deferoxamine on the Repair Ability of Dental Pulp Cells In Vitro</title><author>Jiang, Long, DDS, PhD ; Peng, Wei-Wei, DDS, MDS ; Li, Li-Fen, DDS, PhD ; Du, Rong, DDS, PhD ; Wu, Tian-Tian, DDS, MDS ; Zhou, Zhuo-Jun, DDS, MDS ; Zhao, Jun-Jun, DDS, PhD ; Yang, Ya, DDS, PhD ; Qu, Dong-Lin, BDS ; Zhu, Ya-Qin, DDS, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c547t-c294d845d7c7f612954bb102e484cda2e3c0e55b76155962f1df5cb1212da9e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adolescent</topic><topic>Alkaline Phosphatase - analysis</topic><topic>Calcification, Physiologic - drug effects</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Hypoxia - drug effects</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Coloring Agents</topic><topic>Deferoxamine</topic><topic>Deferoxamine - pharmacology</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - drug effects</topic><topic>dental pulp cells</topic><topic>Dentistry</topic><topic>Endocrinology & Metabolism</topic><topic>Fluorescent Dyes</topic><topic>Humans</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - analysis</topic><topic>hypoxia-inducible factors-1α</topic><topic>Odontoblasts - drug effects</topic><topic>Osteocalcin - analysis</topic><topic>Regeneration - drug effects</topic><topic>repair ability</topic><topic>Siderophores - pharmacology</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Long, DDS, PhD</creatorcontrib><creatorcontrib>Peng, Wei-Wei, DDS, MDS</creatorcontrib><creatorcontrib>Li, Li-Fen, DDS, PhD</creatorcontrib><creatorcontrib>Du, Rong, DDS, PhD</creatorcontrib><creatorcontrib>Wu, Tian-Tian, DDS, MDS</creatorcontrib><creatorcontrib>Zhou, Zhuo-Jun, DDS, MDS</creatorcontrib><creatorcontrib>Zhao, Jun-Jun, DDS, PhD</creatorcontrib><creatorcontrib>Yang, Ya, DDS, PhD</creatorcontrib><creatorcontrib>Qu, Dong-Lin, BDS</creatorcontrib><creatorcontrib>Zhu, Ya-Qin, DDS, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Long, DDS, PhD</au><au>Peng, Wei-Wei, DDS, MDS</au><au>Li, Li-Fen, DDS, PhD</au><au>Du, Rong, DDS, PhD</au><au>Wu, Tian-Tian, DDS, MDS</au><au>Zhou, Zhuo-Jun, DDS, MDS</au><au>Zhao, Jun-Jun, DDS, PhD</au><au>Yang, Ya, DDS, PhD</au><au>Qu, Dong-Lin, BDS</au><au>Zhu, Ya-Qin, DDS, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Deferoxamine on the Repair Ability of Dental Pulp Cells In Vitro</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>40</volume><issue>8</issue><spage>1100</spage><epage>1104</epage><pages>1100-1104</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract Introduction In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs. Methods DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting. Results Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO. Conclusions These results suggest that DFO might improve the repair ability of DPCs by HIF-1α.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25069915</pmid><doi>10.1016/j.joen.2013.12.016</doi><tpages>5</tpages></addata></record> |
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| subjects | Adolescent Alkaline Phosphatase - analysis Calcification, Physiologic - drug effects Cell Culture Techniques Cell Differentiation - drug effects Cell Hypoxia - drug effects Cell Movement - drug effects Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Coloring Agents Deferoxamine Deferoxamine - pharmacology Dental Pulp - cytology Dental Pulp - drug effects dental pulp cells Dentistry Endocrinology & Metabolism Fluorescent Dyes Humans Hypoxia-Inducible Factor 1, alpha Subunit - analysis hypoxia-inducible factors-1α Odontoblasts - drug effects Osteocalcin - analysis Regeneration - drug effects repair ability Siderophores - pharmacology Tetrazolium Salts Thiazoles Young Adult |
| title | Effects of Deferoxamine on the Repair Ability of Dental Pulp Cells In Vitro |
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