Inhibitory Effects of Areca Nut Extract on Expression of Complement Receptors and Fc Receptors in Human Neutrophils
Background: Chewing of areca quid increases the prevalence of periodontal diseases. Areca nut extract (ANE) inhibits the phagocytic activity of human neutrophils. This in vitro study investigates the effects of ANE on complement‐ and antibody‐opsonized phagocytosis by neutrophils. Expression of comp...
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| Veröffentlicht in: | Journal of periodontology (1970) 2014-08, Vol.85 (8), p.1096-1106 |
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| container_title | Journal of periodontology (1970) |
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| creator | Lee, Ya‐Yun Lin, Ming‐Bin Cheng, Chi‐Fang Chang, Lien‐Yu Liu, Tsung‐Yun Hung, Shan‐Ling |
| description | Background: Chewing of areca quid increases the prevalence of periodontal diseases. Areca nut extract (ANE) inhibits the phagocytic activity of human neutrophils. This in vitro study investigates the effects of ANE on complement‐ and antibody‐opsonized phagocytosis by neutrophils. Expression of complement receptors, Fc receptors, and F‐actin in ANE‐treated neutrophils is also analyzed.
Methods: The viability of ANE‐treated neutrophils was determined using the propidium iodide staining method. The possible effects of ANE on the expression of complement receptors and Fc receptors were examined using an immunofluorescence staining method followed by flow cytometry and confocal laser scanning microscopy. The phagocytic activity of neutrophils against complement or immunoglobulin (Ig)G‐opsonized fluorescent beads was analyzed using flow cytometry. Expression of F‐actin was determined using confocal laser scanning microscopy.
Results: ANE significantly inhibited the production of complement receptors (CR1, CR3, and CR4) and Fc receptors (FcγRII and FcγRIII) in a concentration‐dependent manner. Treatment of neutrophils with ANE significantly impaired their ability to phagocytose fluorescent beads. ANE also inhibited phagocytosis of fluorescent beads that were opsonized by complement or IgG. Moreover, expression of F‐actin was inhibited after ANE treatment.
Conclusions: ANE inhibits the complement‐ and IgG‐mediated neutrophil phagocytosis that may result from reduction of the expression of complement receptors, Fc receptors, and F‐actin formation after ANE treatment. The findings suggest that areca nut chewing may jeopardize the defensive functions of neutrophils and affect periodontal health. |
| doi_str_mv | 10.1902/jop.2013.130498 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1549631399</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1549631399</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3436-7f7fb2be249666d45233bdc7eb5d633a47d2ce0aede6f4a1e7f4152f3bef24f03</originalsourceid><addsrcrecordid>eNqFkM9LwzAUgIMoOn-cvUmOXjqTvLS1RxmbU0RF9BzS9IVV2qYmLbr_3oxN8eYpL-HLx-Mj5JyzKS-YuHp3_VQwDlMOTBbXe2TCCwkJZDnbJxPGhEhAFuKIHIfwHq9cAjskR0JCKrOUTUi461Z1WQ_Or-ncWjRDoM7SG49G08dxoPOvwWszUNfFsfcYQh3HiMxc2zfYYjfQFzTYR0Wguqvowvx5qDu6HFvd0UccB-_6Vd2EU3JgdRPwbHeekLfF_HW2TB6ebu9mNw-JAQlZktvclqJEIYssyyqZCoCyMjmWaZUBaJlXwiDTWGFmpeaYW8lTYaFEK6RlcEIut97eu48Rw6DaOhhsGt2hG4PiaTQDh6KI6NUWNd6F4NGq3tet9mvFmdqUVrG02pRW29Lxx8VOPpYtVr_8T9oIpFvgs25w_Z9P3T_PXziL-3wDh1qLeQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1549631399</pqid></control><display><type>article</type><title>Inhibitory Effects of Areca Nut Extract on Expression of Complement Receptors and Fc Receptors in Human Neutrophils</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Lee, Ya‐Yun ; Lin, Ming‐Bin ; Cheng, Chi‐Fang ; Chang, Lien‐Yu ; Liu, Tsung‐Yun ; Hung, Shan‐Ling</creator><creatorcontrib>Lee, Ya‐Yun ; Lin, Ming‐Bin ; Cheng, Chi‐Fang ; Chang, Lien‐Yu ; Liu, Tsung‐Yun ; Hung, Shan‐Ling</creatorcontrib><description>Background: Chewing of areca quid increases the prevalence of periodontal diseases. Areca nut extract (ANE) inhibits the phagocytic activity of human neutrophils. This in vitro study investigates the effects of ANE on complement‐ and antibody‐opsonized phagocytosis by neutrophils. Expression of complement receptors, Fc receptors, and F‐actin in ANE‐treated neutrophils is also analyzed.
Methods: The viability of ANE‐treated neutrophils was determined using the propidium iodide staining method. The possible effects of ANE on the expression of complement receptors and Fc receptors were examined using an immunofluorescence staining method followed by flow cytometry and confocal laser scanning microscopy. The phagocytic activity of neutrophils against complement or immunoglobulin (Ig)G‐opsonized fluorescent beads was analyzed using flow cytometry. Expression of F‐actin was determined using confocal laser scanning microscopy.
Results: ANE significantly inhibited the production of complement receptors (CR1, CR3, and CR4) and Fc receptors (FcγRII and FcγRIII) in a concentration‐dependent manner. Treatment of neutrophils with ANE significantly impaired their ability to phagocytose fluorescent beads. ANE also inhibited phagocytosis of fluorescent beads that were opsonized by complement or IgG. Moreover, expression of F‐actin was inhibited after ANE treatment.
Conclusions: ANE inhibits the complement‐ and IgG‐mediated neutrophil phagocytosis that may result from reduction of the expression of complement receptors, Fc receptors, and F‐actin formation after ANE treatment. The findings suggest that areca nut chewing may jeopardize the defensive functions of neutrophils and affect periodontal health.</description><identifier>ISSN: 0022-3492</identifier><identifier>EISSN: 1943-3670</identifier><identifier>DOI: 10.1902/jop.2013.130498</identifier><identifier>PMID: 24354650</identifier><language>eng</language><publisher>United States: American Academy of Periodontology</publisher><subject>Actins ; Actins - drug effects ; Adult ; Areca ; Cell Survival - drug effects ; Cells, Cultured ; Coloring Agents ; complement ; Complement C1 - drug effects ; Dentistry ; Female ; Flow Cytometry ; Fluorescent Antibody Technique, Direct ; Humans ; Integrin alphaXbeta2 - drug effects ; Macrophage-1 Antigen - drug effects ; Male ; Microscopy, Confocal ; Microspheres ; neutrophils ; Neutrophils - drug effects ; Neutrophils - immunology ; Nuts ; phagocytosis ; Phagocytosis - drug effects ; Plant Extracts - pharmacology ; Propidium ; receptors ; Receptors, Complement - drug effects ; Receptors, Fc - drug effects ; Receptors, IgG - drug effects ; Young Adult</subject><ispartof>Journal of periodontology (1970), 2014-08, Vol.85 (8), p.1096-1106</ispartof><rights>2014 American Academy of Periodontology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3436-7f7fb2be249666d45233bdc7eb5d633a47d2ce0aede6f4a1e7f4152f3bef24f03</citedby><cites>FETCH-LOGICAL-c3436-7f7fb2be249666d45233bdc7eb5d633a47d2ce0aede6f4a1e7f4152f3bef24f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1902%2Fjop.2013.130498$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1902%2Fjop.2013.130498$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24354650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Ya‐Yun</creatorcontrib><creatorcontrib>Lin, Ming‐Bin</creatorcontrib><creatorcontrib>Cheng, Chi‐Fang</creatorcontrib><creatorcontrib>Chang, Lien‐Yu</creatorcontrib><creatorcontrib>Liu, Tsung‐Yun</creatorcontrib><creatorcontrib>Hung, Shan‐Ling</creatorcontrib><title>Inhibitory Effects of Areca Nut Extract on Expression of Complement Receptors and Fc Receptors in Human Neutrophils</title><title>Journal of periodontology (1970)</title><addtitle>J Periodontol</addtitle><description>Background: Chewing of areca quid increases the prevalence of periodontal diseases. Areca nut extract (ANE) inhibits the phagocytic activity of human neutrophils. This in vitro study investigates the effects of ANE on complement‐ and antibody‐opsonized phagocytosis by neutrophils. Expression of complement receptors, Fc receptors, and F‐actin in ANE‐treated neutrophils is also analyzed.
Methods: The viability of ANE‐treated neutrophils was determined using the propidium iodide staining method. The possible effects of ANE on the expression of complement receptors and Fc receptors were examined using an immunofluorescence staining method followed by flow cytometry and confocal laser scanning microscopy. The phagocytic activity of neutrophils against complement or immunoglobulin (Ig)G‐opsonized fluorescent beads was analyzed using flow cytometry. Expression of F‐actin was determined using confocal laser scanning microscopy.
Results: ANE significantly inhibited the production of complement receptors (CR1, CR3, and CR4) and Fc receptors (FcγRII and FcγRIII) in a concentration‐dependent manner. Treatment of neutrophils with ANE significantly impaired their ability to phagocytose fluorescent beads. ANE also inhibited phagocytosis of fluorescent beads that were opsonized by complement or IgG. Moreover, expression of F‐actin was inhibited after ANE treatment.
Conclusions: ANE inhibits the complement‐ and IgG‐mediated neutrophil phagocytosis that may result from reduction of the expression of complement receptors, Fc receptors, and F‐actin formation after ANE treatment. The findings suggest that areca nut chewing may jeopardize the defensive functions of neutrophils and affect periodontal health.</description><subject>Actins</subject><subject>Actins - drug effects</subject><subject>Adult</subject><subject>Areca</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Coloring Agents</subject><subject>complement</subject><subject>Complement C1 - drug effects</subject><subject>Dentistry</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique, Direct</subject><subject>Humans</subject><subject>Integrin alphaXbeta2 - drug effects</subject><subject>Macrophage-1 Antigen - drug effects</subject><subject>Male</subject><subject>Microscopy, Confocal</subject><subject>Microspheres</subject><subject>neutrophils</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - immunology</subject><subject>Nuts</subject><subject>phagocytosis</subject><subject>Phagocytosis - drug effects</subject><subject>Plant Extracts - pharmacology</subject><subject>Propidium</subject><subject>receptors</subject><subject>Receptors, Complement - drug effects</subject><subject>Receptors, Fc - drug effects</subject><subject>Receptors, IgG - drug effects</subject><subject>Young Adult</subject><issn>0022-3492</issn><issn>1943-3670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUgIMoOn-cvUmOXjqTvLS1RxmbU0RF9BzS9IVV2qYmLbr_3oxN8eYpL-HLx-Mj5JyzKS-YuHp3_VQwDlMOTBbXe2TCCwkJZDnbJxPGhEhAFuKIHIfwHq9cAjskR0JCKrOUTUi461Z1WQ_Or-ncWjRDoM7SG49G08dxoPOvwWszUNfFsfcYQh3HiMxc2zfYYjfQFzTYR0Wguqvowvx5qDu6HFvd0UccB-_6Vd2EU3JgdRPwbHeekLfF_HW2TB6ebu9mNw-JAQlZktvclqJEIYssyyqZCoCyMjmWaZUBaJlXwiDTWGFmpeaYW8lTYaFEK6RlcEIut97eu48Rw6DaOhhsGt2hG4PiaTQDh6KI6NUWNd6F4NGq3tet9mvFmdqUVrG02pRW29Lxx8VOPpYtVr_8T9oIpFvgs25w_Z9P3T_PXziL-3wDh1qLeQ</recordid><startdate>201408</startdate><enddate>201408</enddate><creator>Lee, Ya‐Yun</creator><creator>Lin, Ming‐Bin</creator><creator>Cheng, Chi‐Fang</creator><creator>Chang, Lien‐Yu</creator><creator>Liu, Tsung‐Yun</creator><creator>Hung, Shan‐Ling</creator><general>American Academy of Periodontology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201408</creationdate><title>Inhibitory Effects of Areca Nut Extract on Expression of Complement Receptors and Fc Receptors in Human Neutrophils</title><author>Lee, Ya‐Yun ; Lin, Ming‐Bin ; Cheng, Chi‐Fang ; Chang, Lien‐Yu ; Liu, Tsung‐Yun ; Hung, Shan‐Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3436-7f7fb2be249666d45233bdc7eb5d633a47d2ce0aede6f4a1e7f4152f3bef24f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Actins</topic><topic>Actins - drug effects</topic><topic>Adult</topic><topic>Areca</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Coloring Agents</topic><topic>complement</topic><topic>Complement C1 - drug effects</topic><topic>Dentistry</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique, Direct</topic><topic>Humans</topic><topic>Integrin alphaXbeta2 - drug effects</topic><topic>Macrophage-1 Antigen - drug effects</topic><topic>Male</topic><topic>Microscopy, Confocal</topic><topic>Microspheres</topic><topic>neutrophils</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - immunology</topic><topic>Nuts</topic><topic>phagocytosis</topic><topic>Phagocytosis - drug effects</topic><topic>Plant Extracts - pharmacology</topic><topic>Propidium</topic><topic>receptors</topic><topic>Receptors, Complement - drug effects</topic><topic>Receptors, Fc - drug effects</topic><topic>Receptors, IgG - drug effects</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Ya‐Yun</creatorcontrib><creatorcontrib>Lin, Ming‐Bin</creatorcontrib><creatorcontrib>Cheng, Chi‐Fang</creatorcontrib><creatorcontrib>Chang, Lien‐Yu</creatorcontrib><creatorcontrib>Liu, Tsung‐Yun</creatorcontrib><creatorcontrib>Hung, Shan‐Ling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontology (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Ya‐Yun</au><au>Lin, Ming‐Bin</au><au>Cheng, Chi‐Fang</au><au>Chang, Lien‐Yu</au><au>Liu, Tsung‐Yun</au><au>Hung, Shan‐Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effects of Areca Nut Extract on Expression of Complement Receptors and Fc Receptors in Human Neutrophils</atitle><jtitle>Journal of periodontology (1970)</jtitle><addtitle>J Periodontol</addtitle><date>2014-08</date><risdate>2014</risdate><volume>85</volume><issue>8</issue><spage>1096</spage><epage>1106</epage><pages>1096-1106</pages><issn>0022-3492</issn><eissn>1943-3670</eissn><abstract>Background: Chewing of areca quid increases the prevalence of periodontal diseases. Areca nut extract (ANE) inhibits the phagocytic activity of human neutrophils. This in vitro study investigates the effects of ANE on complement‐ and antibody‐opsonized phagocytosis by neutrophils. Expression of complement receptors, Fc receptors, and F‐actin in ANE‐treated neutrophils is also analyzed.
Methods: The viability of ANE‐treated neutrophils was determined using the propidium iodide staining method. The possible effects of ANE on the expression of complement receptors and Fc receptors were examined using an immunofluorescence staining method followed by flow cytometry and confocal laser scanning microscopy. The phagocytic activity of neutrophils against complement or immunoglobulin (Ig)G‐opsonized fluorescent beads was analyzed using flow cytometry. Expression of F‐actin was determined using confocal laser scanning microscopy.
Results: ANE significantly inhibited the production of complement receptors (CR1, CR3, and CR4) and Fc receptors (FcγRII and FcγRIII) in a concentration‐dependent manner. Treatment of neutrophils with ANE significantly impaired their ability to phagocytose fluorescent beads. ANE also inhibited phagocytosis of fluorescent beads that were opsonized by complement or IgG. Moreover, expression of F‐actin was inhibited after ANE treatment.
Conclusions: ANE inhibits the complement‐ and IgG‐mediated neutrophil phagocytosis that may result from reduction of the expression of complement receptors, Fc receptors, and F‐actin formation after ANE treatment. The findings suggest that areca nut chewing may jeopardize the defensive functions of neutrophils and affect periodontal health.</abstract><cop>United States</cop><pub>American Academy of Periodontology</pub><pmid>24354650</pmid><doi>10.1902/jop.2013.130498</doi><tpages>11</tpages></addata></record> |
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| subjects | Actins Actins - drug effects Adult Areca Cell Survival - drug effects Cells, Cultured Coloring Agents complement Complement C1 - drug effects Dentistry Female Flow Cytometry Fluorescent Antibody Technique, Direct Humans Integrin alphaXbeta2 - drug effects Macrophage-1 Antigen - drug effects Male Microscopy, Confocal Microspheres neutrophils Neutrophils - drug effects Neutrophils - immunology Nuts phagocytosis Phagocytosis - drug effects Plant Extracts - pharmacology Propidium receptors Receptors, Complement - drug effects Receptors, Fc - drug effects Receptors, IgG - drug effects Young Adult |
| title | Inhibitory Effects of Areca Nut Extract on Expression of Complement Receptors and Fc Receptors in Human Neutrophils |
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