Neutrophil activation by surface bound IgG is via a pertussis toxin insensitive G protein
Pre-treatment of neutrophils with either pertussis or cholera toxins does not inhibit neutrophil activation by surface bound IgG. In contrast, pretreatment with the phorbol ester, phorbol myristate acetate, results in a dose dependent inhibition of degranulation by surface bound IgG. This inhibition...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1989-11, Vol.164 (3), p.983-989 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Blackburn, Warren D. Heck, Louis W. |
| description | Pre-treatment of neutrophils with either pertussis or cholera toxins does not inhibit neutrophil activation by surface bound IgG. In contrast, pretreatment with the phorbol ester, phorbol myristate acetate, results in a dose dependent inhibition of degranulation by surface bound IgG. This inhibition is similar to that seen with soluble ligands where it is thought to be due to interference with the interaction of an activated guanine nucleotide binding protein with phospholipase C (J. Biol. Chem.,
262
, 6121, 1987). More directly, GTP binding and GTPase activity are enhanced when human neutrophil membranes are incubated in wells containing surface bound IgG. Neither of these G protein functions were inhibited when membranes were prepared in the presence of pertussis toxin, suggesting that neutrophil activation by surface bound IgG proceeds by a mechanism that involves a pertussis toxin insensitive G protein. |
| doi_str_mv | 10.1016/0006-291X(89)91766-X |
| format | Article |
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262
, 6121, 1987). More directly, GTP binding and GTPase activity are enhanced when human neutrophil membranes are incubated in wells containing surface bound IgG. Neither of these G protein functions were inhibited when membranes were prepared in the presence of pertussis toxin, suggesting that neutrophil activation by surface bound IgG proceeds by a mechanism that involves a pertussis toxin insensitive G protein.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(89)91766-X</identifier><identifier>PMID: 2556130</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Cell Membrane - immunology ; Cell Membrane - metabolism ; Cholera Toxin - pharmacology ; GTP Phosphohydrolases - blood ; GTP-Binding Proteins - blood ; Humans ; Immunoglobulin G - physiology ; In Vitro Techniques ; Kinetics ; Lactoferrin - blood ; Neutrophils - immunology ; Neutrophils - physiology ; Pertussis Toxin ; Receptors, Antigen, B-Cell - physiology ; Superoxides - blood ; Tetradecanoylphorbol Acetate - pharmacology ; Virulence Factors, Bordetella - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 1989-11, Vol.164 (3), p.983-989</ispartof><rights>1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-fa03f1a7a7e32c30cfea99f02774fd5f0a5c7d19e016068d7c60f29305c38533</citedby><cites>FETCH-LOGICAL-c388t-fa03f1a7a7e32c30cfea99f02774fd5f0a5c7d19e016068d7c60f29305c38533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X8991766X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2556130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blackburn, Warren D.</creatorcontrib><creatorcontrib>Heck, Louis W.</creatorcontrib><title>Neutrophil activation by surface bound IgG is via a pertussis toxin insensitive G protein</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Pre-treatment of neutrophils with either pertussis or cholera toxins does not inhibit neutrophil activation by surface bound IgG. In contrast, pretreatment with the phorbol ester, phorbol myristate acetate, results in a dose dependent inhibition of degranulation by surface bound IgG. This inhibition is similar to that seen with soluble ligands where it is thought to be due to interference with the interaction of an activated guanine nucleotide binding protein with phospholipase C (J. Biol. Chem.,
262
, 6121, 1987). More directly, GTP binding and GTPase activity are enhanced when human neutrophil membranes are incubated in wells containing surface bound IgG. Neither of these G protein functions were inhibited when membranes were prepared in the presence of pertussis toxin, suggesting that neutrophil activation by surface bound IgG proceeds by a mechanism that involves a pertussis toxin insensitive G protein.</description><subject>Adult</subject><subject>Cell Membrane - immunology</subject><subject>Cell Membrane - metabolism</subject><subject>Cholera Toxin - pharmacology</subject><subject>GTP Phosphohydrolases - blood</subject><subject>GTP-Binding Proteins - blood</subject><subject>Humans</subject><subject>Immunoglobulin G - physiology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Lactoferrin - blood</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - physiology</subject><subject>Pertussis Toxin</subject><subject>Receptors, Antigen, B-Cell - physiology</subject><subject>Superoxides - blood</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Virulence Factors, Bordetella - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LAzEQxYMotVa_gUJOoofVyWb_5SJI0VooeumhnkKanWik3a1Jtthvb2pLj56G4b03j_kRcsngjgEr7gGgSFLBZjeVuBWsLIpkdkT6DAQkKYPsmPQPllNy5v0XAGNZIXqkl-Z5wTj0yfsrdsG1q0-7oEoHu1bBtg2db6jvnFEa6bztmpqOP0bUerq2iiq6Qhc67-Me2h_bUNt4bLyNaaQjunJtQNuckxOjFh4v9nNAps9P0-FLMnkbjYePk0TzqgqJUcANU6UqkaeagzaohDCQlmVm6tyAynVZM4HxZSiqutQFmFRwyGM-53xArndnY-13hz7IpfUaFwvVYNt5yfJMAGdpNGY7o3at9w6NXDm7VG4jGcgtULmlJbe0ZCXkH1A5i7Gr_f1uvsT6ENoTjPrDTsf449qik15bbDTW1qEOsm7t_wW__ESGBg</recordid><startdate>19891115</startdate><enddate>19891115</enddate><creator>Blackburn, Warren D.</creator><creator>Heck, Louis W.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19891115</creationdate><title>Neutrophil activation by surface bound IgG is via a pertussis toxin insensitive G protein</title><author>Blackburn, Warren D. ; Heck, Louis W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-fa03f1a7a7e32c30cfea99f02774fd5f0a5c7d19e016068d7c60f29305c38533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adult</topic><topic>Cell Membrane - immunology</topic><topic>Cell Membrane - metabolism</topic><topic>Cholera Toxin - pharmacology</topic><topic>GTP Phosphohydrolases - blood</topic><topic>GTP-Binding Proteins - blood</topic><topic>Humans</topic><topic>Immunoglobulin G - physiology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Lactoferrin - blood</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - physiology</topic><topic>Pertussis Toxin</topic><topic>Receptors, Antigen, B-Cell - physiology</topic><topic>Superoxides - blood</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Virulence Factors, Bordetella - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blackburn, Warren D.</creatorcontrib><creatorcontrib>Heck, Louis W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blackburn, Warren D.</au><au>Heck, Louis W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutrophil activation by surface bound IgG is via a pertussis toxin insensitive G protein</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1989-11-15</date><risdate>1989</risdate><volume>164</volume><issue>3</issue><spage>983</spage><epage>989</epage><pages>983-989</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Pre-treatment of neutrophils with either pertussis or cholera toxins does not inhibit neutrophil activation by surface bound IgG. In contrast, pretreatment with the phorbol ester, phorbol myristate acetate, results in a dose dependent inhibition of degranulation by surface bound IgG. This inhibition is similar to that seen with soluble ligands where it is thought to be due to interference with the interaction of an activated guanine nucleotide binding protein with phospholipase C (J. Biol. Chem.,
262
, 6121, 1987). More directly, GTP binding and GTPase activity are enhanced when human neutrophil membranes are incubated in wells containing surface bound IgG. Neither of these G protein functions were inhibited when membranes were prepared in the presence of pertussis toxin, suggesting that neutrophil activation by surface bound IgG proceeds by a mechanism that involves a pertussis toxin insensitive G protein.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2556130</pmid><doi>10.1016/0006-291X(89)91766-X</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1989-11, Vol.164 (3), p.983-989 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Cell Membrane - immunology Cell Membrane - metabolism Cholera Toxin - pharmacology GTP Phosphohydrolases - blood GTP-Binding Proteins - blood Humans Immunoglobulin G - physiology In Vitro Techniques Kinetics Lactoferrin - blood Neutrophils - immunology Neutrophils - physiology Pertussis Toxin Receptors, Antigen, B-Cell - physiology Superoxides - blood Tetradecanoylphorbol Acetate - pharmacology Virulence Factors, Bordetella - pharmacology |
| title | Neutrophil activation by surface bound IgG is via a pertussis toxin insensitive G protein |
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