Profile of PRMT-1 gene polymorphism in hemodialysis patients with increased ADMA levels
to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients. genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection...
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| Veröffentlicht in: | Acta medica Indonesiana 2014-04, Vol.46 (2), p.97-103 |
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| creator | Thaha, Mochammad Nilamsari, Wenny P Yusuf, Mochammad Amin, M |
| description | to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients.
genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate's single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined.
fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G.
the increased levels of ADMA might be caused by PRMT-1 gene polymorphism. |
| format | Article |
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genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate's single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined.
fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G.
the increased levels of ADMA might be caused by PRMT-1 gene polymorphism.</description><identifier>ISSN: 0125-9326</identifier><identifier>PMID: 25053681</identifier><language>eng</language><publisher>Indonesia</publisher><subject>Arginine - analogs & derivatives ; Arginine - blood ; Cross-Sectional Studies ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Protein-Arginine N-Methyltransferases - genetics ; Renal Dialysis ; Repressor Proteins - genetics ; Sequence Analysis, DNA</subject><ispartof>Acta medica Indonesiana, 2014-04, Vol.46 (2), p.97-103</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25053681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thaha, Mochammad</creatorcontrib><creatorcontrib>Nilamsari, Wenny P</creatorcontrib><creatorcontrib>Yusuf, Mochammad</creatorcontrib><creatorcontrib>Amin, M</creatorcontrib><title>Profile of PRMT-1 gene polymorphism in hemodialysis patients with increased ADMA levels</title><title>Acta medica Indonesiana</title><addtitle>Acta Med Indones</addtitle><description>to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients.
genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate's single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined.
fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G.
the increased levels of ADMA might be caused by PRMT-1 gene polymorphism.</description><subject>Arginine - analogs & derivatives</subject><subject>Arginine - blood</subject><subject>Cross-Sectional Studies</subject><subject>Female</subject><subject>Genotype</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Protein-Arginine N-Methyltransferases - genetics</subject><subject>Renal Dialysis</subject><subject>Repressor Proteins - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>0125-9326</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtqwzAURLVoaUKaXyhadmPQ1Y0teRnSJyQ0lJQujWRfNyqy5Vp2i_--gaargTOHWcwFmwuQaZKjzGZsGeOnEAIEolbZFZvJVKSYaZiz930faueJh5rvX3eHBPgHtcS74Kcm9N3RxYa7lh-pCZUzfoou8s4Mjtoh8h83HE9t2ZOJVPH13W7NPX2Tj9fssjY-0vKcC_b2cH_YPCXbl8fnzXqbGImrISmtFJVE0Ci1reEEbaqkUsYKCVZlus4VAtrMSoloqIQcVqVWoGxJkhAX7PZvt-vD10hxKBoXS_LetBTGWEC60pBLVOqk3pzV0TZUFV3vGtNPxf8Z-AsJQFme</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Thaha, Mochammad</creator><creator>Nilamsari, Wenny P</creator><creator>Yusuf, Mochammad</creator><creator>Amin, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20140401</creationdate><title>Profile of PRMT-1 gene polymorphism in hemodialysis patients with increased ADMA levels</title><author>Thaha, Mochammad ; Nilamsari, Wenny P ; Yusuf, Mochammad ; Amin, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a234t-cb20d2318328bf1a23b57277ab021b768f97313b6b2233aec1914c8717bce2e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Arginine - analogs & derivatives</topic><topic>Arginine - blood</topic><topic>Cross-Sectional Studies</topic><topic>Female</topic><topic>Genotype</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Protein-Arginine N-Methyltransferases - genetics</topic><topic>Renal Dialysis</topic><topic>Repressor Proteins - genetics</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thaha, Mochammad</creatorcontrib><creatorcontrib>Nilamsari, Wenny P</creatorcontrib><creatorcontrib>Yusuf, Mochammad</creatorcontrib><creatorcontrib>Amin, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Acta medica Indonesiana</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thaha, Mochammad</au><au>Nilamsari, Wenny P</au><au>Yusuf, Mochammad</au><au>Amin, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profile of PRMT-1 gene polymorphism in hemodialysis patients with increased ADMA levels</atitle><jtitle>Acta medica Indonesiana</jtitle><addtitle>Acta Med Indones</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>46</volume><issue>2</issue><spage>97</spage><epage>103</epage><pages>97-103</pages><issn>0125-9326</issn><abstract>to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients.
genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate's single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined.
fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G.
the increased levels of ADMA might be caused by PRMT-1 gene polymorphism.</abstract><cop>Indonesia</cop><pmid>25053681</pmid><tpages>7</tpages></addata></record> |
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| ispartof | Acta medica Indonesiana, 2014-04, Vol.46 (2), p.97-103 |
| issn | 0125-9326 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Arginine - analogs & derivatives Arginine - blood Cross-Sectional Studies Female Genotype Humans Male Middle Aged Polymorphism, Single Nucleotide Protein-Arginine N-Methyltransferases - genetics Renal Dialysis Repressor Proteins - genetics Sequence Analysis, DNA |
| title | Profile of PRMT-1 gene polymorphism in hemodialysis patients with increased ADMA levels |
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