Basic and acidic fibroblast growth factors modulate the epidermal growth factor receptor by a protein kinase C-independent pathway

Human acidic and basic fibroblast growth factors (aFGF and bFGF) inhibit epidermal growth factor (EGF) receptor binding in mouse Swiss 3T3 cells. Scatchard analysis indicates that aFGF and bFGF cause a decrease in the high affinity EGF receptor population, similar to that observed for activators of...

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Veröffentlicht in:Biochemical and biophysical research communications 1989-10, Vol.164 (2), p.796-803
Hauptverfasser: Hicks, Karen, Friedman, BethAnn, Rosner, Marsha Rich
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container_title Biochemical and biophysical research communications
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creator Hicks, Karen
Friedman, BethAnn
Rosner, Marsha Rich
description Human acidic and basic fibroblast growth factors (aFGF and bFGF) inhibit epidermal growth factor (EGF) receptor binding in mouse Swiss 3T3 cells. Scatchard analysis indicates that aFGF and bFGF cause a decrease in the high affinity EGF receptor population, similar to that observed for activators of protein kinase C such as phorbol esters, platelet-derived growth factor (PDGF) and bombesin. However, unlike phorbol esters, aFGF and bFGF inhibit EGF binding in protein kinase C-deficient cells. The time course and dose response of inhibition of EGF binding by both aFGF and bFGF are very similar, with an ID 50 of approximately 0.10 ng/ml. In contrast to bombesin but like PDGF, neither aFGF nor bFGF act on the EGF receptor through a pertussis toxinsensitive G protein. These results indicate that both acidic and basic FGF depress high affinity EGF binding in Swiss 3T3 cells with similar potency through a protein kinase C G i -independent pathway.
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subjects Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Bombesin - pharmacology
Cattle
Cells, Cultured
Epidermal Growth Factor - metabolism
ErbB Receptors - drug effects
ErbB Receptors - metabolism
Fibroblast Growth Factors - pharmacology
Fundamental and applied biological sciences. Psychology
Humans
Kinetics
Mice
Pertussis Toxin
Phorbol 12,13-Dibutyrate - pharmacology
Platelet-Derived Growth Factor - pharmacology
Protein hormones. Growth factors. Cytokines
Protein Kinase C - metabolism
Proteins
Virulence Factors, Bordetella - pharmacology
title Basic and acidic fibroblast growth factors modulate the epidermal growth factor receptor by a protein kinase C-independent pathway
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