Improvement of the tetramethyl benzidine reaction with ammonium molybdate as a stabilizer for light and electron microscopic ligand-HRP neurohistochemistry, immunocytochemistry and double-labelling

Ammonium heptamolybdate (AHM) was used as a stabilizing agent in the tetramethyl benzidine (TMB) reaction of choleragen subunit B conjugated horseradish peroxidase (CB-HRP) neurohistochemistry (TMB-AHM method). In comparison with Mesulam's TMB method employing sodium nitroprusside as a stabiliz...

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Veröffentlicht in:	Journal of neuroscience methods 1989-06, Vol.28 (3), p.155-162
Hauptverfasser:	Liang, Fengyi, Wan, Xuancai S.T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium molybdate ammunium heptamolybdate Animals Benzidines Biological and medical sciences Brain Mapping - methods Cholera Toxin choleragen subunit B conjugated horseradish peroxidase Double labelling Electron microscopy Female Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Histocytochemistry - methods Horseradish Peroxidase Immunocytochemistry Immunohistochemistry - methods Male Microscopy, Electron Molybdenum Neurohistochemistry Neurons - analysis Neurons - cytology Peroxidases Rats Rats, Inbred Strains Sodium nitroprusside Tetramethyl benzidine Vertebrates: nervous system and sense organs
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