Consistent oncogene methylation changes in epithelial cells chemically transformed in vitro
We have examined the restriction digest patterns of CCGG sequences in Ki- ras, Ha- ras, and c- myc oncogenes in rat tracheal epithelial cells transformed in vitro by 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene/12-O-tetradecanoylphorbol-13-acetate (TPA), or TPA alone. Oncogenes c- myc and Ha- ras...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1989-10, Vol.164 (2), p.693-699 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Mass, Marc J. Schorschinsky, Nancy S. Lasley, Jessica A. Beeman, Diane K. Austin, Stephen J. |
| description | We have examined the restriction digest patterns of CCGG sequences in Ki-
ras, Ha-
ras, and c-
myc oncogenes in rat tracheal epithelial cells transformed
in vitro by 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene/12-O-tetradecanoylphorbol-13-acetate (TPA), or TPA alone. Oncogenes c-
myc and Ha-
ras in transformed cell lines, compared to normal rat tracheal epithelial cells and untreated primary cultures, had altered Hpa II restriction patterns as demonstrated by hybridizing bands of different molecular weight, or loss of bands. Ki-
ras was hypermethylated in all cell derivations, including normal cells. These molecular alterations have not previously been reported for epithelial cells transformed
in vitro by polycyclic hydrocarbons and tumor promoters, and suggest common mechanisms of action for agents with diverse molecular targets. |
| doi_str_mv | 10.1016/0006-291X(89)91515-5 |
| format | Article |
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ras, Ha-
ras, and c-
myc oncogenes in rat tracheal epithelial cells transformed
in vitro by 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene/12-O-tetradecanoylphorbol-13-acetate (TPA), or TPA alone. Oncogenes c-
myc and Ha-
ras in transformed cell lines, compared to normal rat tracheal epithelial cells and untreated primary cultures, had altered Hpa II restriction patterns as demonstrated by hybridizing bands of different molecular weight, or loss of bands. Ki-
ras was hypermethylated in all cell derivations, including normal cells. These molecular alterations have not previously been reported for epithelial cells transformed
in vitro by polycyclic hydrocarbons and tumor promoters, and suggest common mechanisms of action for agents with diverse molecular targets.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(89)91515-5</identifier><identifier>PMID: 2510720</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>9,10-Dimethyl-1,2-benzanthracene - pharmacology ; Animals ; Base Sequence ; Benzo(a)pyrene - pharmacology ; Biological and medical sciences ; Blotting, Southern ; Cell Line ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Cell Transformation, Neoplastic ; Cells, Cultured ; DNA - drug effects ; DNA - genetics ; Epithelial Cells ; Epithelium - drug effects ; Fundamental and applied biological sciences. Psychology ; Genes, ras - drug effects ; Male ; Methylation ; Molecular and cellular biology ; Proto-Oncogenes - drug effects ; Rats ; Rats, Inbred F344 ; Restriction Mapping ; Tetradecanoylphorbol Acetate - pharmacology ; Trachea</subject><ispartof>Biochemical and biophysical research communications, 1989-10, Vol.164 (2), p.693-699</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-c521b721ac87f09e86cf9b2aef8cbf1c173aae843026c3e9507b04f9af542c913</citedby><cites>FETCH-LOGICAL-c332t-c521b721ac87f09e86cf9b2aef8cbf1c173aae843026c3e9507b04f9af542c913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(89)91515-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6737500$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2510720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mass, Marc J.</creatorcontrib><creatorcontrib>Schorschinsky, Nancy S.</creatorcontrib><creatorcontrib>Lasley, Jessica A.</creatorcontrib><creatorcontrib>Beeman, Diane K.</creatorcontrib><creatorcontrib>Austin, Stephen J.</creatorcontrib><title>Consistent oncogene methylation changes in epithelial cells chemically transformed in vitro</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have examined the restriction digest patterns of CCGG sequences in Ki-
ras, Ha-
ras, and c-
myc oncogenes in rat tracheal epithelial cells transformed
in vitro by 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene/12-O-tetradecanoylphorbol-13-acetate (TPA), or TPA alone. Oncogenes c-
myc and Ha-
ras in transformed cell lines, compared to normal rat tracheal epithelial cells and untreated primary cultures, had altered Hpa II restriction patterns as demonstrated by hybridizing bands of different molecular weight, or loss of bands. Ki-
ras was hypermethylated in all cell derivations, including normal cells. These molecular alterations have not previously been reported for epithelial cells transformed
in vitro by polycyclic hydrocarbons and tumor promoters, and suggest common mechanisms of action for agents with diverse molecular targets.</description><subject>9,10-Dimethyl-1,2-benzanthracene - pharmacology</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Benzo(a)pyrene - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cells, Cultured</subject><subject>DNA - drug effects</subject><subject>DNA - genetics</subject><subject>Epithelial Cells</subject><subject>Epithelium - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, ras - drug effects</subject><subject>Male</subject><subject>Methylation</subject><subject>Molecular and cellular biology</subject><subject>Proto-Oncogenes - drug effects</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Restriction Mapping</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Trachea</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFq3DAQhkVoSTZp3yABH0poD05nZMu2LoWyNG0hkEsLgRyErB1lVWxpK2kX9u1jd5c99jSH__uHmY-xa4Q7BGw-A0BTcolPHzv5SaJAUYoztkCQUHKE-g1bnJALdpnSHwDEupHn7JwLhJbDgj0vg08uZfK5CN6EF_JUjJTX-0FnF3xh1tq_UCqcL2jj8poGp4fC0DCkKaPRGT0M-yJH7ZMNcaTVjO5cjuEde2v1kOj9cV6x3_fffi1_lA-P338uvz6Upqp4Lo3g2LcctelaC5K6xljZc022M71Fg22lNXV1BbwxFUkBbQ-1ldqKmhuJ1RW7PezdxPB3Symr0aX5Qu0pbJNCUTdCtHwC6wNoYkgpklWb6EYd9wpBzU7VLEzNwlQn1T-nSky1m-P-bT_9dyodJU75h2Ou02TDTiqMSyesaatWwIx9OWA0udg5iioZR97QykUyWa2C-_8dr8cflHA</recordid><startdate>19891031</startdate><enddate>19891031</enddate><creator>Mass, Marc J.</creator><creator>Schorschinsky, Nancy S.</creator><creator>Lasley, Jessica A.</creator><creator>Beeman, Diane K.</creator><creator>Austin, Stephen J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19891031</creationdate><title>Consistent oncogene methylation changes in epithelial cells chemically transformed in vitro</title><author>Mass, Marc J. ; Schorschinsky, Nancy S. ; Lasley, Jessica A. ; Beeman, Diane K. ; Austin, Stephen J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-c521b721ac87f09e86cf9b2aef8cbf1c173aae843026c3e9507b04f9af542c913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>9,10-Dimethyl-1,2-benzanthracene - pharmacology</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Benzo(a)pyrene - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cells, Cultured</topic><topic>DNA - drug effects</topic><topic>DNA - genetics</topic><topic>Epithelial Cells</topic><topic>Epithelium - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, ras - drug effects</topic><topic>Male</topic><topic>Methylation</topic><topic>Molecular and cellular biology</topic><topic>Proto-Oncogenes - drug effects</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Restriction Mapping</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Trachea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mass, Marc J.</creatorcontrib><creatorcontrib>Schorschinsky, Nancy S.</creatorcontrib><creatorcontrib>Lasley, Jessica A.</creatorcontrib><creatorcontrib>Beeman, Diane K.</creatorcontrib><creatorcontrib>Austin, Stephen J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mass, Marc J.</au><au>Schorschinsky, Nancy S.</au><au>Lasley, Jessica A.</au><au>Beeman, Diane K.</au><au>Austin, Stephen J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Consistent oncogene methylation changes in epithelial cells chemically transformed in vitro</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1989-10-31</date><risdate>1989</risdate><volume>164</volume><issue>2</issue><spage>693</spage><epage>699</epage><pages>693-699</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>We have examined the restriction digest patterns of CCGG sequences in Ki-
ras, Ha-
ras, and c-
myc oncogenes in rat tracheal epithelial cells transformed
in vitro by 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene/12-O-tetradecanoylphorbol-13-acetate (TPA), or TPA alone. Oncogenes c-
myc and Ha-
ras in transformed cell lines, compared to normal rat tracheal epithelial cells and untreated primary cultures, had altered Hpa II restriction patterns as demonstrated by hybridizing bands of different molecular weight, or loss of bands. Ki-
ras was hypermethylated in all cell derivations, including normal cells. These molecular alterations have not previously been reported for epithelial cells transformed
in vitro by polycyclic hydrocarbons and tumor promoters, and suggest common mechanisms of action for agents with diverse molecular targets.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2510720</pmid><doi>10.1016/0006-291X(89)91515-5</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1989-10, Vol.164 (2), p.693-699 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 9,10-Dimethyl-1,2-benzanthracene - pharmacology Animals Base Sequence Benzo(a)pyrene - pharmacology Biological and medical sciences Blotting, Southern Cell Line Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cell Transformation, Neoplastic Cells, Cultured DNA - drug effects DNA - genetics Epithelial Cells Epithelium - drug effects Fundamental and applied biological sciences. Psychology Genes, ras - drug effects Male Methylation Molecular and cellular biology Proto-Oncogenes - drug effects Rats Rats, Inbred F344 Restriction Mapping Tetradecanoylphorbol Acetate - pharmacology Trachea |
| title | Consistent oncogene methylation changes in epithelial cells chemically transformed in vitro |
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