Crude extracts of yeasts in biocatalysed organic reactions: The example of Pichia pastoris
This paper deals with enzymatic activities present in dialyzed crude extracts of methanol-grown Pichia pastoris: oxidation of NADH by O 2, degradation of NADH, secondary alcohol dehydrogenase and formate dehydrogenase activities. The use of crude extracts of the yeast as a biocatalyst is discussed w...
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| Veröffentlicht in: | Enzyme and microbial technology 1990, Vol.12 (1), p.46-51 |
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| container_title | Enzyme and microbial technology |
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| creator | Haurat, Gisèle Massé, François Souppe, Jérôme |
| description | This paper deals with enzymatic activities present in dialyzed crude extracts of methanol-grown
Pichia pastoris: oxidation of NADH by O
2, degradation of NADH, secondary alcohol dehydrogenase and formate dehydrogenase activities. The use of crude extracts of the yeast as a biocatalyst is discussed with respect to the different enzyme activities in order to choose the best experimental conditions to carry out bioconversions involving NADH or NAD
+ regenerations. |
| doi_str_mv | 10.1016/0141-0229(90)90179-T |
| format | Article |
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Pichia pastoris: oxidation of NADH by O
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Pichia pastoris: oxidation of NADH by O
2, degradation of NADH, secondary alcohol dehydrogenase and formate dehydrogenase activities. The use of crude extracts of the yeast as a biocatalyst is discussed with respect to the different enzyme activities in order to choose the best experimental conditions to carry out bioconversions involving NADH or NAD
+ regenerations.</description><subject>biocatalysis</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>crude extracts</subject><subject>enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Mycology</subject><subject>NAD</subject><subject>Physiology and metabolism</subject><subject>Pichia pastoris</subject><subject>Yeast</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rGzEQhkVJoK7Tf9DDHkJoD5uMvJLW6qEQTL7A0B7cSy9iVjuqVdYrR1qH-N9XGxsfc9JIPM876GXsC4drDlzdABe8hNlMf9XwTQOvdbn6wCZ8ngfQoM_Y5IR8ZJ9S-geQHwRM2J9F3LVU0OsQ0Q6pCK7YE6Y8-b5ofLA4YLdP1BYh_sXe2yJSBn3o0_ditR5N3Gw7GsVf3q49Ftush-jTBTt32CX6fDyn7Pf93WrxWC5_PjwtbpelrZQYyhZEPUMnEZTkDZBUABprqbhzUtO8AaXQacErKQUnrCrRNPlWtdZhy201ZVeH3G0MzztKg9n4ZKnrsKewS4ZLoYSQkEFxAG0MKUVyZhv9BuPecDBjkWZsyYwtGQ3mrUizytrlMR-Txc5F7K1PJ1epeV3XY_qPA0b5ry-eoknWU2-p9ZHsYNrg39_zHzNshyc</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Haurat, Gisèle</creator><creator>Massé, François</creator><creator>Souppe, Jérôme</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>1990</creationdate><title>Crude extracts of yeasts in biocatalysed organic reactions: The example of Pichia pastoris</title><author>Haurat, Gisèle ; Massé, François ; Souppe, Jérôme</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-d0472af5a0651b0e56009a7561ff59e8b066af94135541ea334bb4133dcfad1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>biocatalysis</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>crude extracts</topic><topic>enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Mycology</topic><topic>NAD</topic><topic>Physiology and metabolism</topic><topic>Pichia pastoris</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haurat, Gisèle</creatorcontrib><creatorcontrib>Massé, François</creatorcontrib><creatorcontrib>Souppe, Jérôme</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haurat, Gisèle</au><au>Massé, François</au><au>Souppe, Jérôme</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crude extracts of yeasts in biocatalysed organic reactions: The example of Pichia pastoris</atitle><jtitle>Enzyme and microbial technology</jtitle><date>1990</date><risdate>1990</risdate><volume>12</volume><issue>1</issue><spage>46</spage><epage>51</epage><pages>46-51</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>This paper deals with enzymatic activities present in dialyzed crude extracts of methanol-grown
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2, degradation of NADH, secondary alcohol dehydrogenase and formate dehydrogenase activities. The use of crude extracts of the yeast as a biocatalyst is discussed with respect to the different enzyme activities in order to choose the best experimental conditions to carry out bioconversions involving NADH or NAD
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| fulltext | fulltext |
| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 1990, Vol.12 (1), p.46-51 |
| issn | 0141-0229 1879-0909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15464450 |
| source | Elsevier ScienceDirect Journals Complete |
| subjects | biocatalysis Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology crude extracts enzymes Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Microbiology Mission oriented research Mycology NAD Physiology and metabolism Pichia pastoris Yeast |
| title | Crude extracts of yeasts in biocatalysed organic reactions: The example of Pichia pastoris |
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