Development and application of a loop-mediated isothermal amplification assay for rapid detection of Pythium helicoides

Abstract Root rot of poinsettia, caused by Pythium helicoides at high temperatures in hydroponic cultures, has become a serious problem in many parts of the world. We have developed a species-specific, loop-mediated isothermal amplification (LAMP) assay for the rapid diagnosis of this pathogen. The...

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Veröffentlicht in:	FEMS microbiology letters 2014-06, Vol.355 (1), p.28-35
Hauptverfasser:	Takahashi, Reiko, Fukuta, Shiro, Kuroyanagi, Satoru, Miyake, Noriyuki, Nagai, Hirofumi, Kageyama, Koji, Ishiguro, Yasushi
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA Primers - genetics DNA, Ribosomal Spacer - genetics Euphorbia - microbiology High temperature hydroponic culture Hydroponics loop‐mediated isothermal amplification Microbiology Nucleic Acid Amplification Techniques - methods Oomycetes - genetics Oomycetes - isolation & purification Pathogens Plant Diseases - microbiology Plant Roots - microbiology Pythium helicoides Sensitivity and Specificity
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