Overexpression, purification, and characterization of Paenibacillus cell surface-expressed chitinase ChiW with two catalytic domains

Paenibacillus sp. strain FPU-7 produces several different chitinases and effectively hydrolyzes robust chitin. Among the P. FPU-7 chitinases, ChiW, a novel monomeric chitinase with a molecular mass of 150 kDa, is expressed as a cell surface molecule. Here, we report that active ChiW lacking the anch...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2014, Vol.78 (4), p.624-634
Hauptverfasser:	Itoh, Takafumi, Sugimoto, Ikumi, Hibi, Takao, Suzuki, Fumiko, Matsuo, Koichi, Fujii, Yutaka, Taketo, Akira, Kimoto, Hisashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Catalytic Domain cell surface-expressed chitinase chitin Chitin - metabolism Chitinases - chemistry Chitinases - genetics Chitinases - isolation & purification Chitinases - metabolism Escherichia coli - genetics Gene Expression glycoside hydrolase family 18 Molecular Sequence Data multi modular protein Mutagenesis Mutation Paenibacillus - enzymology Paenibacillus sp. FPU-7 Substrate Specificity
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