Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii)
Some snakes have several anti-toxic proteins in their sera that neutralize their own venom. Five new small serum proteins (SSPs) were isolated from Japanese mamushi (Gloydius blomhoffii) serum by gel-filtration and RP-HPLC, and their N-Terminal sequences were determined. The amino acid sequences of...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2014-01, Vol.78 (3), p.410-419 |
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| creator | Shioi, Narumi Deshimaru, Masanobu Terada, Shigeyuki |
| description | Some snakes have several anti-toxic proteins in their sera that neutralize their own venom. Five new small serum proteins (SSPs) were isolated from Japanese mamushi (Gloydius blomhoffii) serum by gel-filtration and RP-HPLC, and their N-Terminal sequences were determined. The amino acid sequences of the precursor proteins were deduced from the nucleotide sequences of cDNAs encoding them. Due to the sequence similarity to those of SSPs in habu snake (Protobothrops flavoviridis) serum (>75% identity), these proteins were designated mSSP-1 to mSSP-5 as the homologs of habu proteins. mSSP-1 was stable at 100 °C and in the pH range of 1-10, and inhibited the proteolytic activity of a certain snake venom metalloproteinase. The inhibitory activity was extinguished by modifying the amino groups of mSSP-1. mSSP-1 is the first prostate secretory protein of the 94 amino acid-family protein with a carbohydrate chain in the Asn37 residue.
Sequence Alignment of PSP94-Family Proteins. G. blomhoffii and P. flavoviridis SSPs are respectively designated by mSSPs and hSSPs. A glycosylation site in mSSP-1 is underlined. |
| doi_str_mv | 10.1080/09168451.2014.890030 |
| format | Article |
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Sequence Alignment of PSP94-Family Proteins. G. blomhoffii and P. flavoviridis SSPs are respectively designated by mSSPs and hSSPs. A glycosylation site in mSSP-1 is underlined.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1080/09168451.2014.890030</identifier><identifier>PMID: 25036827</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Animals ; Base Sequence ; Blood Proteins - chemistry ; Blood Proteins - genetics ; Blood Proteins - isolation & purification ; DNA, Complementary - genetics ; endogenous inhibitor ; self-defense system ; Sequence Homology, Amino Acid ; snake venom metalloproteinase inhibitor ; Snake Venoms - chemistry ; Snake Venoms - genetics ; Trimeresurus - blood ; venomous snake serum protein</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2014-01, Vol.78 (3), p.410-419</ispartof><rights>2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-f128d106e0b5b3c49081884bac2b23062d1d091df5603776a55dd3aecddf4ed23</citedby><cites>FETCH-LOGICAL-c493t-f128d106e0b5b3c49081884bac2b23062d1d091df5603776a55dd3aecddf4ed23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25036827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shioi, Narumi</creatorcontrib><creatorcontrib>Deshimaru, Masanobu</creatorcontrib><creatorcontrib>Terada, Shigeyuki</creatorcontrib><title>Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii)</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Some snakes have several anti-toxic proteins in their sera that neutralize their own venom. Five new small serum proteins (SSPs) were isolated from Japanese mamushi (Gloydius blomhoffii) serum by gel-filtration and RP-HPLC, and their N-Terminal sequences were determined. The amino acid sequences of the precursor proteins were deduced from the nucleotide sequences of cDNAs encoding them. Due to the sequence similarity to those of SSPs in habu snake (Protobothrops flavoviridis) serum (>75% identity), these proteins were designated mSSP-1 to mSSP-5 as the homologs of habu proteins. mSSP-1 was stable at 100 °C and in the pH range of 1-10, and inhibited the proteolytic activity of a certain snake venom metalloproteinase. The inhibitory activity was extinguished by modifying the amino groups of mSSP-1. mSSP-1 is the first prostate secretory protein of the 94 amino acid-family protein with a carbohydrate chain in the Asn37 residue.
Sequence Alignment of PSP94-Family Proteins. G. blomhoffii and P. flavoviridis SSPs are respectively designated by mSSPs and hSSPs. A glycosylation site in mSSP-1 is underlined.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Blood Proteins - chemistry</subject><subject>Blood Proteins - genetics</subject><subject>Blood Proteins - isolation & purification</subject><subject>DNA, Complementary - genetics</subject><subject>endogenous inhibitor</subject><subject>self-defense system</subject><subject>Sequence Homology, Amino Acid</subject><subject>snake venom metalloproteinase inhibitor</subject><subject>Snake Venoms - chemistry</subject><subject>Snake Venoms - genetics</subject><subject>Trimeresurus - blood</subject><subject>venomous snake serum protein</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEuLFDEUhYMoTjv6D0SyHBfVc_OoR69EBh2FARfqOqTyoKN5tEnKoQX_u2mqx6W4SnL4zrk3B6GXBLYEJriGHRkm3pMtBcK30w6AwSO0IYyP3bDj42O0OSHdiblAz0r5BtCEnjxFF7QHNkx03KDfn2teVF2y9FhG6Y_FlXbRWO1llqqa7H7J6lLEyeJo7nEJ0ntcTF4CPuRUjYsF25wCrntz1hsq8U8TU0hLwSXK7wZf3fp01K69Z5_CPlnr3Ovn6ImVvpgX5_MSfX3_7svNh-7u0-3Hm7d3neI7VjtL6KQJDAbmfmZNg4lME5-lojNlMFBNdPuatv0AbBwH2fdaM2mU1pYbTdklulpz28Y_FlOqCK4o472Mpq0oSM8HShhwaChfUZVTKdlYccguyHwUBMSpePFQvDgVL9bim-3VecIyB6P_mh6absD1CqTl8L-Rb1aHizblIO9T9lpUefQp2yyjckWwfyb8ATU5oWM</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Shioi, Narumi</creator><creator>Deshimaru, Masanobu</creator><creator>Terada, Shigeyuki</creator><general>Taylor & Francis</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140101</creationdate><title>Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii)</title><author>Shioi, Narumi ; Deshimaru, Masanobu ; Terada, Shigeyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-f128d106e0b5b3c49081884bac2b23062d1d091df5603776a55dd3aecddf4ed23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Blood Proteins - chemistry</topic><topic>Blood Proteins - genetics</topic><topic>Blood Proteins - isolation & purification</topic><topic>DNA, Complementary - genetics</topic><topic>endogenous inhibitor</topic><topic>self-defense system</topic><topic>Sequence Homology, Amino Acid</topic><topic>snake venom metalloproteinase inhibitor</topic><topic>Snake Venoms - chemistry</topic><topic>Snake Venoms - genetics</topic><topic>Trimeresurus - blood</topic><topic>venomous snake serum protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shioi, Narumi</creatorcontrib><creatorcontrib>Deshimaru, Masanobu</creatorcontrib><creatorcontrib>Terada, Shigeyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shioi, Narumi</au><au>Deshimaru, Masanobu</au><au>Terada, Shigeyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii)</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>78</volume><issue>3</issue><spage>410</spage><epage>419</epage><pages>410-419</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Some snakes have several anti-toxic proteins in their sera that neutralize their own venom. Five new small serum proteins (SSPs) were isolated from Japanese mamushi (Gloydius blomhoffii) serum by gel-filtration and RP-HPLC, and their N-Terminal sequences were determined. The amino acid sequences of the precursor proteins were deduced from the nucleotide sequences of cDNAs encoding them. Due to the sequence similarity to those of SSPs in habu snake (Protobothrops flavoviridis) serum (>75% identity), these proteins were designated mSSP-1 to mSSP-5 as the homologs of habu proteins. mSSP-1 was stable at 100 °C and in the pH range of 1-10, and inhibited the proteolytic activity of a certain snake venom metalloproteinase. The inhibitory activity was extinguished by modifying the amino groups of mSSP-1. mSSP-1 is the first prostate secretory protein of the 94 amino acid-family protein with a carbohydrate chain in the Asn37 residue.
Sequence Alignment of PSP94-Family Proteins. G. blomhoffii and P. flavoviridis SSPs are respectively designated by mSSPs and hSSPs. A glycosylation site in mSSP-1 is underlined.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>25036827</pmid><doi>10.1080/09168451.2014.890030</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2014-01, Vol.78 (3), p.410-419 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Base Sequence Blood Proteins - chemistry Blood Proteins - genetics Blood Proteins - isolation & purification DNA, Complementary - genetics endogenous inhibitor self-defense system Sequence Homology, Amino Acid snake venom metalloproteinase inhibitor Snake Venoms - chemistry Snake Venoms - genetics Trimeresurus - blood venomous snake serum protein |
| title | Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii) |
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