An electron spin resonance study of a novel radical cation produced during the horseradish peroxidase-catalyzed oxidation of tetramethylhydrazine
The one-electron oxidation of tetramethylhydrazine by the horseradish peroxidase/hydrogen peroxide system forms a novel stable radical cation. The steady-state concentration of the tetramethylhydrazine radical cation increases with the hydrazine concentration, but depends on neither the enzyme conce...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1982-03, Vol.105 (1), p.217-224 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kalyanaraman, B. Mason, Ronald P. Sivarajah, K. |
| description | The one-electron oxidation of tetramethylhydrazine by the horseradish peroxidase/hydrogen peroxide system forms a novel stable radical cation. The steady-state concentration of the tetramethylhydrazine radical cation increases with the hydrazine concentration, but depends on neither the enzyme concentration nor the hydrogen peroxide concentration. A mechanism involving the enzymatic one-electron oxidation of the tetramethylhydrazine radical cation to a dication is proposed to account for the enzyme independency. Incubations containing horseradish peroxidase, hydrogen peroxide and tetramethylhydrazine produced formaldehyde which was found to increase with either HRP or tetramethylhydrazine concentration. The cation radical, the dication, and the imine (the deprotonated dication) are proposed as intermediates in the mechanism of formaldehyde production. |
| doi_str_mv | 10.1016/S0006-291X(82)80033-8 |
| format | Article |
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The steady-state concentration of the tetramethylhydrazine radical cation increases with the hydrazine concentration, but depends on neither the enzyme concentration nor the hydrogen peroxide concentration. A mechanism involving the enzymatic one-electron oxidation of the tetramethylhydrazine radical cation to a dication is proposed to account for the enzyme independency. Incubations containing horseradish peroxidase, hydrogen peroxide and tetramethylhydrazine produced formaldehyde which was found to increase with either HRP or tetramethylhydrazine concentration. 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| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1982-03, Vol.105 (1), p.217-224 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15456849 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Armoracia lapathifolia Carcinogens E.S.R Electron Spin Resonance Spectroscopy Free Radicals Horseradish Peroxidase - metabolism Kinetics Methylhydrazines Oxidation-Reduction peroxidase Peroxidases - metabolism tetramethylhydrazine |
| title | An electron spin resonance study of a novel radical cation produced during the horseradish peroxidase-catalyzed oxidation of tetramethylhydrazine |
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