Study of the fast‐reacting cysteines in phosphoglycerate kinase using chemical modification and site‐directed mutagenesis

Horse muscle phosphoglycerate kinase, like other mammalian phosphoglycerate kinases, contains seven cysteine residues of which two react rapidly with 5,5′‐dithio‐bis(2‐nitrobenzoate) (Nbs2) following second‐order kinetics (k= 640 M−1· s−1). Selective cyanylation of the fast‐reacting cysteines, follo...

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