Immunobiological properties of 1-butanol-extracted cell surface antigens
Extracts of viable 3-methylcholanthrene-induced murine sarcoma cells (MCA-F and MCA-2A) prepared using single-phase (2.5%) 1-butanol significantly retarded the outgrowth of the homotypic, but not the heterotypic, tumor of syngeneic C3H/HeJ mice. Butanol extracts specifically evoked a delayed hyperse...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1981-10, Vol.41 (10), p.3956-3960 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | LeGrue, S J Allison, J P Macek, C M Pellis, N R Kahan, B D |
| description | Extracts of viable 3-methylcholanthrene-induced murine sarcoma cells (MCA-F and MCA-2A) prepared using single-phase (2.5%) 1-butanol significantly retarded the outgrowth of the homotypic, but not the heterotypic, tumor of syngeneic C3H/HeJ mice. Butanol extracts specifically evoked a delayed hypersensitivity response in tumor-immune syngeneic mice, but not in alloimmune DBA/2J mice. Crude butanol extracts of MCA-F cells did not contain alloantigenic activity, as shown by their inability to block H-2 or Ia-specific antibodies in a complement-dependent cytotoxicity assay. Absorption of these same allospecific reagents with untreated or with butanol-extracted cells indicated that H-2 antigens remain associated with the cell surface during extraction. Thus, butanol appears to release tumor antigens, but not alloantigens, from the cell surface. |
| format | Article |
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Butanol extracts specifically evoked a delayed hypersensitivity response in tumor-immune syngeneic mice, but not in alloimmune DBA/2J mice. Crude butanol extracts of MCA-F cells did not contain alloantigenic activity, as shown by their inability to block H-2 or Ia-specific antibodies in a complement-dependent cytotoxicity assay. Absorption of these same allospecific reagents with untreated or with butanol-extracted cells indicated that H-2 antigens remain associated with the cell surface during extraction. Thus, butanol appears to release tumor antigens, but not alloantigens, from the cell surface.</description><identifier>ISSN: 0008-5472</identifier><identifier>PMID: 7026027</identifier><language>eng</language><publisher>United States</publisher><subject>1-butanol ; Animals ; antigens ; Antigens, Neoplasm - immunology ; Antigens, Neoplasm - isolation & purification ; Butanols ; cell surface ; Female ; Fibrosarcoma - chemically induced ; Fibrosarcoma - immunology ; Immunologic Techniques ; Methylcholanthrene ; Mice ; Mice, Inbred C3H ; Mice, Inbred DBA ; Sarcoma, Experimental - chemically induced ; Sarcoma, Experimental - immunology ; tumor cells</subject><ispartof>Cancer research (Chicago, Ill.), 1981-10, Vol.41 (10), p.3956-3960</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7026027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LeGrue, S J</creatorcontrib><creatorcontrib>Allison, J P</creatorcontrib><creatorcontrib>Macek, C M</creatorcontrib><creatorcontrib>Pellis, N R</creatorcontrib><creatorcontrib>Kahan, B D</creatorcontrib><title>Immunobiological properties of 1-butanol-extracted cell surface antigens</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Extracts of viable 3-methylcholanthrene-induced murine sarcoma cells (MCA-F and MCA-2A) prepared using single-phase (2.5%) 1-butanol significantly retarded the outgrowth of the homotypic, but not the heterotypic, tumor of syngeneic C3H/HeJ mice. Butanol extracts specifically evoked a delayed hypersensitivity response in tumor-immune syngeneic mice, but not in alloimmune DBA/2J mice. Crude butanol extracts of MCA-F cells did not contain alloantigenic activity, as shown by their inability to block H-2 or Ia-specific antibodies in a complement-dependent cytotoxicity assay. Absorption of these same allospecific reagents with untreated or with butanol-extracted cells indicated that H-2 antigens remain associated with the cell surface during extraction. Thus, butanol appears to release tumor antigens, but not alloantigens, from the cell surface.</description><subject>1-butanol</subject><subject>Animals</subject><subject>antigens</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Antigens, Neoplasm - isolation & purification</subject><subject>Butanols</subject><subject>cell surface</subject><subject>Female</subject><subject>Fibrosarcoma - chemically induced</subject><subject>Fibrosarcoma - immunology</subject><subject>Immunologic Techniques</subject><subject>Methylcholanthrene</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mice, Inbred DBA</subject><subject>Sarcoma, Experimental - chemically induced</subject><subject>Sarcoma, Experimental - immunology</subject><subject>tumor cells</subject><issn>0008-5472</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotj01LxDAURbNQxnH0JwhduSskadKmSxnUGRiYja7LS_syRtKm5gP031uxq8uBy-XcK7KllKpSiobfkNsYPxeUjMoN2TSU15Q3W3I4jmOevLbe-YvtwRVz8DOGZDEW3hSs1DnB5F2J3ylAn3AoenSuiDkY6LGAKdkLTvGOXBtwEe_X3JH3l-e3_aE8nV-P-6dT-cEblkrTgqG8UgY0NKyumNZgFqqZAC2MFKDYsABlyJWmALyWPWWqRTO0TEG1I4__u4vnV8aYutHGPyOY0OfYMSmE4kItxYe1mPWIQzcHO0L46dbr1S-_LVUB</recordid><startdate>19811001</startdate><enddate>19811001</enddate><creator>LeGrue, S J</creator><creator>Allison, J P</creator><creator>Macek, C M</creator><creator>Pellis, N R</creator><creator>Kahan, B D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>19811001</creationdate><title>Immunobiological properties of 1-butanol-extracted cell surface antigens</title><author>LeGrue, S J ; Allison, J P ; Macek, C M ; Pellis, N R ; Kahan, B D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h271t-f9af0238faba71631bbaf8fa614ab4f54a81d61401e28b0aa265c0189efd918a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>1-butanol</topic><topic>Animals</topic><topic>antigens</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Antigens, Neoplasm - isolation & purification</topic><topic>Butanols</topic><topic>cell surface</topic><topic>Female</topic><topic>Fibrosarcoma - chemically induced</topic><topic>Fibrosarcoma - immunology</topic><topic>Immunologic Techniques</topic><topic>Methylcholanthrene</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Mice, Inbred DBA</topic><topic>Sarcoma, Experimental - chemically induced</topic><topic>Sarcoma, Experimental - immunology</topic><topic>tumor cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LeGrue, S J</creatorcontrib><creatorcontrib>Allison, J P</creatorcontrib><creatorcontrib>Macek, C M</creatorcontrib><creatorcontrib>Pellis, N R</creatorcontrib><creatorcontrib>Kahan, B D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LeGrue, S J</au><au>Allison, J P</au><au>Macek, C M</au><au>Pellis, N R</au><au>Kahan, B D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunobiological properties of 1-butanol-extracted cell surface antigens</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1981-10-01</date><risdate>1981</risdate><volume>41</volume><issue>10</issue><spage>3956</spage><epage>3960</epage><pages>3956-3960</pages><issn>0008-5472</issn><abstract>Extracts of viable 3-methylcholanthrene-induced murine sarcoma cells (MCA-F and MCA-2A) prepared using single-phase (2.5%) 1-butanol significantly retarded the outgrowth of the homotypic, but not the heterotypic, tumor of syngeneic C3H/HeJ mice. Butanol extracts specifically evoked a delayed hypersensitivity response in tumor-immune syngeneic mice, but not in alloimmune DBA/2J mice. Crude butanol extracts of MCA-F cells did not contain alloantigenic activity, as shown by their inability to block H-2 or Ia-specific antibodies in a complement-dependent cytotoxicity assay. Absorption of these same allospecific reagents with untreated or with butanol-extracted cells indicated that H-2 antigens remain associated with the cell surface during extraction. Thus, butanol appears to release tumor antigens, but not alloantigens, from the cell surface.</abstract><cop>United States</cop><pmid>7026027</pmid><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0008-5472 |
| ispartof | Cancer research (Chicago, Ill.), 1981-10, Vol.41 (10), p.3956-3960 |
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| language | eng |
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| source | MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 1-butanol Animals antigens Antigens, Neoplasm - immunology Antigens, Neoplasm - isolation & purification Butanols cell surface Female Fibrosarcoma - chemically induced Fibrosarcoma - immunology Immunologic Techniques Methylcholanthrene Mice Mice, Inbred C3H Mice, Inbred DBA Sarcoma, Experimental - chemically induced Sarcoma, Experimental - immunology tumor cells |
| title | Immunobiological properties of 1-butanol-extracted cell surface antigens |
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