Enhanced survival of transplanted human adipose-derived stem cells by co-delivery with liposomal apoptosome inhibitor in fibrin gel matrix
To improve the survival of transplanted human adipose-derived stem cells (ADSCs), a liposome preparation containing the apoptosome inhibitor, NS3694, was formulated and co-delivered with ADSCs in fibrin gel scaffolds. Liposomes provided enhanced effect on ADSC proliferation in vitro as compared to f...
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| Veröffentlicht in: | European journal of pharmaceutics and biopharmaceutics 2013-11, Vol.85 (3), p.673-681 |
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| creator | Shim, Gayong Im, Saewon Lee, Sangbin Park, Joo Yeon Kim, Jinyoung Jin, Hyerim Lee, Soondong Im, Irang Kim, Dae-Duk Kim, Seong Who Lee, Taik Jong Eom, Jin Sup Yi, Tac-Ghee Song, Sun Uk Byun, Youngro Oh, Yu-Kyoung |
| description | To improve the survival of transplanted human adipose-derived stem cells (ADSCs), a liposome preparation containing the apoptosome inhibitor, NS3694, was formulated and co-delivered with ADSCs in fibrin gel scaffolds. Liposomes provided enhanced effect on ADSC proliferation in vitro as compared to free drug. Exposure of ADSCs to liposomal NS3694 for 7days did not affect the surface marker expression profile. NS3694 encapsulated in negatively charged liposomes composed of phosphatidylcholine, phosphatidylglycerol, and cholesterol was evaluated in vivo following subcutaneous transplantation in mice. Survival of ADSCs co-delivered with liposomal NS3694 was significantly higher than that of untreated ADSCs or ADSCs treated with free NS3694 or empty liposomes. An immunohistochemical analysis revealed a higher number of human nucleus-positive cells after treatment with liposomal NS3694 than following treatment with free NS3694. Similarly, liposomal NS3694 significantly enhanced survival of transplanted ADSCs in rabbits compared to other treatments. Taken together, our results indicate the potential of liposomal NS3694 co-delivered with ADSCs using fibrin gel systems as an in vivo-survival enhancer. |
| doi_str_mv | 10.1016/j.ejpb.2013.05.014 |
| format | Article |
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Liposomes provided enhanced effect on ADSC proliferation in vitro as compared to free drug. Exposure of ADSCs to liposomal NS3694 for 7days did not affect the surface marker expression profile. NS3694 encapsulated in negatively charged liposomes composed of phosphatidylcholine, phosphatidylglycerol, and cholesterol was evaluated in vivo following subcutaneous transplantation in mice. Survival of ADSCs co-delivered with liposomal NS3694 was significantly higher than that of untreated ADSCs or ADSCs treated with free NS3694 or empty liposomes. An immunohistochemical analysis revealed a higher number of human nucleus-positive cells after treatment with liposomal NS3694 than following treatment with free NS3694. Similarly, liposomal NS3694 significantly enhanced survival of transplanted ADSCs in rabbits compared to other treatments. Taken together, our results indicate the potential of liposomal NS3694 co-delivered with ADSCs using fibrin gel systems as an in vivo-survival enhancer.</description><identifier>ISSN: 0939-6411</identifier><identifier>EISSN: 1873-3441</identifier><identifier>DOI: 10.1016/j.ejpb.2013.05.014</identifier><identifier>PMID: 23747743</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adipose Tissue - cytology ; Animals ; Apoptosome inhibitor ; Apoptosomes - antagonists & inhibitors ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Co-delivery ; Female ; Fibrin - chemistry ; Gels ; Human adipose tissue-derived stem cells ; Humans ; Immunohistochemistry ; Liposomes ; Mice ; Mice, Nude ; ortho-Aminobenzoates - administration & dosage ; ortho-Aminobenzoates - pharmacology ; Phenylurea Compounds - administration & dosage ; Phenylurea Compounds - pharmacology ; Rabbits ; Stem Cell Transplantation ; Stem Cells - cytology ; Survival ; Transplantation</subject><ispartof>European journal of pharmaceutics and biopharmaceutics, 2013-11, Vol.85 (3), p.673-681</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-c03ddc02cc1a25224cf5313d1142664e75a2b48fb3278e153396cc79185f024e3</citedby><cites>FETCH-LOGICAL-c389t-c03ddc02cc1a25224cf5313d1142664e75a2b48fb3278e153396cc79185f024e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejpb.2013.05.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23747743$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shim, Gayong</creatorcontrib><creatorcontrib>Im, Saewon</creatorcontrib><creatorcontrib>Lee, Sangbin</creatorcontrib><creatorcontrib>Park, Joo Yeon</creatorcontrib><creatorcontrib>Kim, Jinyoung</creatorcontrib><creatorcontrib>Jin, Hyerim</creatorcontrib><creatorcontrib>Lee, Soondong</creatorcontrib><creatorcontrib>Im, Irang</creatorcontrib><creatorcontrib>Kim, Dae-Duk</creatorcontrib><creatorcontrib>Kim, Seong Who</creatorcontrib><creatorcontrib>Lee, Taik Jong</creatorcontrib><creatorcontrib>Eom, Jin Sup</creatorcontrib><creatorcontrib>Yi, Tac-Ghee</creatorcontrib><creatorcontrib>Song, Sun Uk</creatorcontrib><creatorcontrib>Byun, Youngro</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><title>Enhanced survival of transplanted human adipose-derived stem cells by co-delivery with liposomal apoptosome inhibitor in fibrin gel matrix</title><title>European journal of pharmaceutics and biopharmaceutics</title><addtitle>Eur J Pharm Biopharm</addtitle><description>To improve the survival of transplanted human adipose-derived stem cells (ADSCs), a liposome preparation containing the apoptosome inhibitor, NS3694, was formulated and co-delivered with ADSCs in fibrin gel scaffolds. 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Taken together, our results indicate the potential of liposomal NS3694 co-delivered with ADSCs using fibrin gel systems as an in vivo-survival enhancer.</description><subject>Adipose Tissue - cytology</subject><subject>Animals</subject><subject>Apoptosome inhibitor</subject><subject>Apoptosomes - antagonists & inhibitors</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Co-delivery</subject><subject>Female</subject><subject>Fibrin - chemistry</subject><subject>Gels</subject><subject>Human adipose tissue-derived stem cells</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Liposomes</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>ortho-Aminobenzoates - administration & dosage</subject><subject>ortho-Aminobenzoates - pharmacology</subject><subject>Phenylurea Compounds - administration & dosage</subject><subject>Phenylurea Compounds - pharmacology</subject><subject>Rabbits</subject><subject>Stem Cell Transplantation</subject><subject>Stem Cells - cytology</subject><subject>Survival</subject><subject>Transplantation</subject><issn>0939-6411</issn><issn>1873-3441</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EokPbF2CBvGST4ONLLhIbVLWlUiU2sLYc54TxKImD7QzMK_DUOJrCkq7Okf7v_JL9EfIWWAkMqg-HEg9LV3IGomSqZCBfkB00tSiElPCS7Fgr2qKSABfkTYwHxpisVfOaXHBRy7qWYkd-3857M1vsaVzD0R3NSP1AUzBzXEYzpxzs18nM1PRu8RGLHoM7bnjCiVocx0i7E7U-B2MOwon-dGlPx432U64zi1_StiN18951LvmQNzq4LuTxHUc6mRTcryvyajBjxOuneUm-3d1-vflcPH65f7j59FhY0bSpsEz0vWXcWjBccS7toASIHkDyqpJYK8M72Qyd4HWDoIRoK2vrFho1MC5RXJL3594l-B8rxqQnF7eXmBn9GjUoKRnUlWifR2XFoWlBVRnlZ9QGH2PAQS_BTSacNDC96dIHvenSmy7NlM668tG7p_61m7D_d_LXTwY-ngHMH3J0GHS0DjdfLqBNuvfuf_1_AEXJqCg</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Shim, Gayong</creator><creator>Im, Saewon</creator><creator>Lee, Sangbin</creator><creator>Park, Joo Yeon</creator><creator>Kim, Jinyoung</creator><creator>Jin, Hyerim</creator><creator>Lee, Soondong</creator><creator>Im, Irang</creator><creator>Kim, Dae-Duk</creator><creator>Kim, Seong Who</creator><creator>Lee, Taik Jong</creator><creator>Eom, Jin Sup</creator><creator>Yi, Tac-Ghee</creator><creator>Song, Sun Uk</creator><creator>Byun, Youngro</creator><creator>Oh, Yu-Kyoung</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20131101</creationdate><title>Enhanced survival of transplanted human adipose-derived stem cells by co-delivery with liposomal apoptosome inhibitor in fibrin gel matrix</title><author>Shim, Gayong ; Im, Saewon ; Lee, Sangbin ; Park, Joo Yeon ; Kim, Jinyoung ; Jin, Hyerim ; Lee, Soondong ; Im, Irang ; Kim, Dae-Duk ; Kim, Seong Who ; Lee, Taik Jong ; Eom, Jin Sup ; Yi, Tac-Ghee ; Song, Sun Uk ; Byun, Youngro ; Oh, Yu-Kyoung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-c03ddc02cc1a25224cf5313d1142664e75a2b48fb3278e153396cc79185f024e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adipose Tissue - cytology</topic><topic>Animals</topic><topic>Apoptosome inhibitor</topic><topic>Apoptosomes - antagonists & inhibitors</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Co-delivery</topic><topic>Female</topic><topic>Fibrin - chemistry</topic><topic>Gels</topic><topic>Human adipose tissue-derived stem cells</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Liposomes</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>ortho-Aminobenzoates - administration & dosage</topic><topic>ortho-Aminobenzoates - pharmacology</topic><topic>Phenylurea Compounds - administration & dosage</topic><topic>Phenylurea Compounds - pharmacology</topic><topic>Rabbits</topic><topic>Stem Cell Transplantation</topic><topic>Stem Cells - cytology</topic><topic>Survival</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shim, Gayong</creatorcontrib><creatorcontrib>Im, Saewon</creatorcontrib><creatorcontrib>Lee, Sangbin</creatorcontrib><creatorcontrib>Park, Joo Yeon</creatorcontrib><creatorcontrib>Kim, Jinyoung</creatorcontrib><creatorcontrib>Jin, Hyerim</creatorcontrib><creatorcontrib>Lee, Soondong</creatorcontrib><creatorcontrib>Im, Irang</creatorcontrib><creatorcontrib>Kim, Dae-Duk</creatorcontrib><creatorcontrib>Kim, Seong Who</creatorcontrib><creatorcontrib>Lee, Taik Jong</creatorcontrib><creatorcontrib>Eom, Jin Sup</creatorcontrib><creatorcontrib>Yi, Tac-Ghee</creatorcontrib><creatorcontrib>Song, Sun Uk</creatorcontrib><creatorcontrib>Byun, Youngro</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>European journal of pharmaceutics and biopharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shim, Gayong</au><au>Im, Saewon</au><au>Lee, Sangbin</au><au>Park, Joo Yeon</au><au>Kim, Jinyoung</au><au>Jin, Hyerim</au><au>Lee, Soondong</au><au>Im, Irang</au><au>Kim, Dae-Duk</au><au>Kim, Seong Who</au><au>Lee, Taik Jong</au><au>Eom, Jin Sup</au><au>Yi, Tac-Ghee</au><au>Song, Sun Uk</au><au>Byun, Youngro</au><au>Oh, Yu-Kyoung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced survival of transplanted human adipose-derived stem cells by co-delivery with liposomal apoptosome inhibitor in fibrin gel matrix</atitle><jtitle>European journal of pharmaceutics and biopharmaceutics</jtitle><addtitle>Eur J Pharm Biopharm</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>85</volume><issue>3</issue><spage>673</spage><epage>681</epage><pages>673-681</pages><issn>0939-6411</issn><eissn>1873-3441</eissn><abstract>To improve the survival of transplanted human adipose-derived stem cells (ADSCs), a liposome preparation containing the apoptosome inhibitor, NS3694, was formulated and co-delivered with ADSCs in fibrin gel scaffolds. Liposomes provided enhanced effect on ADSC proliferation in vitro as compared to free drug. Exposure of ADSCs to liposomal NS3694 for 7days did not affect the surface marker expression profile. NS3694 encapsulated in negatively charged liposomes composed of phosphatidylcholine, phosphatidylglycerol, and cholesterol was evaluated in vivo following subcutaneous transplantation in mice. Survival of ADSCs co-delivered with liposomal NS3694 was significantly higher than that of untreated ADSCs or ADSCs treated with free NS3694 or empty liposomes. An immunohistochemical analysis revealed a higher number of human nucleus-positive cells after treatment with liposomal NS3694 than following treatment with free NS3694. Similarly, liposomal NS3694 significantly enhanced survival of transplanted ADSCs in rabbits compared to other treatments. Taken together, our results indicate the potential of liposomal NS3694 co-delivered with ADSCs using fibrin gel systems as an in vivo-survival enhancer.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23747743</pmid><doi>10.1016/j.ejpb.2013.05.014</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0939-6411 |
| ispartof | European journal of pharmaceutics and biopharmaceutics, 2013-11, Vol.85 (3), p.673-681 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adipose Tissue - cytology Animals Apoptosome inhibitor Apoptosomes - antagonists & inhibitors Cell Proliferation - drug effects Cell Survival - drug effects Co-delivery Female Fibrin - chemistry Gels Human adipose tissue-derived stem cells Humans Immunohistochemistry Liposomes Mice Mice, Nude ortho-Aminobenzoates - administration & dosage ortho-Aminobenzoates - pharmacology Phenylurea Compounds - administration & dosage Phenylurea Compounds - pharmacology Rabbits Stem Cell Transplantation Stem Cells - cytology Survival Transplantation |
| title | Enhanced survival of transplanted human adipose-derived stem cells by co-delivery with liposomal apoptosome inhibitor in fibrin gel matrix |
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