The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription
•Chlorella accumulate considerable lipid during heterotrophic to autotrophic transition.•Lipid and protein contents increased rapidly along with the sharp decline of starch.•Isogenes encoding ME, PEPC and DGAT are related to lipid accumulation.•These genes might be exploited as target genes for gene...
Gespeichert in:
| Veröffentlicht in: | Bioresource technology 2014-07, Vol.164, p.214-220 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 220 |
|---|---|
| container_issue | |
| container_start_page | 214 |
| container_title | Bioresource technology |
| container_volume | 164 |
| creator | Fan, Jianhua Cui, Yanbin Zhou, Yang Wan, Minxi Wang, Weiliang Xie, Jingli Li, Yuanguang |
| description | •Chlorella accumulate considerable lipid during heterotrophic to autotrophic transition.•Lipid and protein contents increased rapidly along with the sharp decline of starch.•Isogenes encoding ME, PEPC and DGAT are related to lipid accumulation.•These genes might be exploited as target genes for genetic modification.•The switch strategy could be developed for efficient algal lipid production.
Heterotrophy to photoautotrophy transition leads to the accumulation of lipids in Chlorella, which has potential to produce both healthy food and biofuels. Therefore, it is of key interest to study the metabolism shift and gene expression changes that influenced by the transition. Both total and neutral lipids contents were increased rapidly within 48h after the switch to light environment, from 24.5% and 18.0% to 35.3% and 27.4%, respectively, along with the sharp decline of starch from 42.3% to 10.4% during 24h photoinduction phase. By analyzing the correlation between lipid content and gene expression, results revealed several genes viz. me g3137, me g6562, pepc g6833, dgat g3280 and dgat g7566, which encode corresponding enzymes in the de novo lipid biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. These results represented the feasibility of lipid production through trophic converting cultivation. |
| doi_str_mv | 10.1016/j.biortech.2014.04.087 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1544013562</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960852414006270</els_id><sourcerecordid>1534796212</sourcerecordid><originalsourceid>FETCH-LOGICAL-c497t-e13339eb17f90f11c823ce08d7861838bab950ae16d31de68b8949614fb6b6103</originalsourceid><addsrcrecordid>eNqNkcFu1DAQhi0Eotstr1D5gsSBbD1x4tg30AooUqVe2rPlOJPGq2wcbC_Vvj0Ou4UjSL80kvV5PJ6PkGtgG2Agbnab1vmQ0A6bkkG1YTmyeUVWIBtelKoRr8mKKcEKWZfVBbmMcccY49CUb8lFWclalcBX5PgwIMW-R5uo7-l0SMEl5yc6m5QwTNSMuZjfRznbYfQBx9HQ-Rhw8q7z0dCn4J_T8JGObnYdzYPF45QGjC4WGTYJO_qEE9IUzBRtcPPS7oq86c0Y8d25rsnj1y8P29vi7v7b9-3nu8JWqkkFAudcYQtNr1gPYGXJLTLZNVKA5LI1raqZQRAdhw6FbKWqlICqb0UrgPE1-XDqOwf_44Ax6b2LdvnDhP4QNdRVxYDXovwPlFeNEiUsqDihNvgYA_Z6Dm5vwlED04shvdMvhvRiSLOc7GZNrs9vHNo9dn-uvSjJwPszYKI1Y593Zl38y2Wfktcyc59OHObl_XQYdLQOJ4udC9mm7rz71yy_AHEUtF0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1534796212</pqid></control><display><type>article</type><title>The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Fan, Jianhua ; Cui, Yanbin ; Zhou, Yang ; Wan, Minxi ; Wang, Weiliang ; Xie, Jingli ; Li, Yuanguang</creator><creatorcontrib>Fan, Jianhua ; Cui, Yanbin ; Zhou, Yang ; Wan, Minxi ; Wang, Weiliang ; Xie, Jingli ; Li, Yuanguang</creatorcontrib><description>•Chlorella accumulate considerable lipid during heterotrophic to autotrophic transition.•Lipid and protein contents increased rapidly along with the sharp decline of starch.•Isogenes encoding ME, PEPC and DGAT are related to lipid accumulation.•These genes might be exploited as target genes for genetic modification.•The switch strategy could be developed for efficient algal lipid production.
Heterotrophy to photoautotrophy transition leads to the accumulation of lipids in Chlorella, which has potential to produce both healthy food and biofuels. Therefore, it is of key interest to study the metabolism shift and gene expression changes that influenced by the transition. Both total and neutral lipids contents were increased rapidly within 48h after the switch to light environment, from 24.5% and 18.0% to 35.3% and 27.4%, respectively, along with the sharp decline of starch from 42.3% to 10.4% during 24h photoinduction phase. By analyzing the correlation between lipid content and gene expression, results revealed several genes viz. me g3137, me g6562, pepc g6833, dgat g3280 and dgat g7566, which encode corresponding enzymes in the de novo lipid biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. These results represented the feasibility of lipid production through trophic converting cultivation.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2014.04.087</identifier><identifier>PMID: 24859213</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Biosynthetic Pathways - drug effects ; Biosynthetic Pathways - genetics ; Chlorella ; Chlorella - drug effects ; Chlorella - genetics ; Chlorella - growth & development ; Chlorella - metabolism ; Diacylglycerol O-Acyltransferase - genetics ; Diacylglycerol O-Acyltransferase - metabolism ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation - drug effects ; Genes ; Genetic modification ; Heterotrophic Processes - drug effects ; Heterotrophic Processes - genetics ; Heterotrophy ; Intracellular Space - drug effects ; Intracellular Space - metabolism ; Lipid ; Lipid Metabolism - drug effects ; Lipids ; Lipids - biosynthesis ; Nitrogen - pharmacology ; Nutrition ; Phosphorus - pharmacology ; Photoautotrophy ; Phototrophic Processes - drug effects ; Phototrophic Processes - genetics ; Real-Time Polymerase Chain Reaction ; Starches ; Transcription, Genetic - drug effects</subject><ispartof>Bioresource technology, 2014-07, Vol.164, p.214-220</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-e13339eb17f90f11c823ce08d7861838bab950ae16d31de68b8949614fb6b6103</citedby><cites>FETCH-LOGICAL-c497t-e13339eb17f90f11c823ce08d7861838bab950ae16d31de68b8949614fb6b6103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2014.04.087$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28528358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24859213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, Jianhua</creatorcontrib><creatorcontrib>Cui, Yanbin</creatorcontrib><creatorcontrib>Zhou, Yang</creatorcontrib><creatorcontrib>Wan, Minxi</creatorcontrib><creatorcontrib>Wang, Weiliang</creatorcontrib><creatorcontrib>Xie, Jingli</creatorcontrib><creatorcontrib>Li, Yuanguang</creatorcontrib><title>The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•Chlorella accumulate considerable lipid during heterotrophic to autotrophic transition.•Lipid and protein contents increased rapidly along with the sharp decline of starch.•Isogenes encoding ME, PEPC and DGAT are related to lipid accumulation.•These genes might be exploited as target genes for genetic modification.•The switch strategy could be developed for efficient algal lipid production.
Heterotrophy to photoautotrophy transition leads to the accumulation of lipids in Chlorella, which has potential to produce both healthy food and biofuels. Therefore, it is of key interest to study the metabolism shift and gene expression changes that influenced by the transition. Both total and neutral lipids contents were increased rapidly within 48h after the switch to light environment, from 24.5% and 18.0% to 35.3% and 27.4%, respectively, along with the sharp decline of starch from 42.3% to 10.4% during 24h photoinduction phase. By analyzing the correlation between lipid content and gene expression, results revealed several genes viz. me g3137, me g6562, pepc g6833, dgat g3280 and dgat g7566, which encode corresponding enzymes in the de novo lipid biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. These results represented the feasibility of lipid production through trophic converting cultivation.</description><subject>Biological and medical sciences</subject><subject>Biosynthetic Pathways - drug effects</subject><subject>Biosynthetic Pathways - genetics</subject><subject>Chlorella</subject><subject>Chlorella - drug effects</subject><subject>Chlorella - genetics</subject><subject>Chlorella - growth & development</subject><subject>Chlorella - metabolism</subject><subject>Diacylglycerol O-Acyltransferase - genetics</subject><subject>Diacylglycerol O-Acyltransferase - metabolism</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes</subject><subject>Genetic modification</subject><subject>Heterotrophic Processes - drug effects</subject><subject>Heterotrophic Processes - genetics</subject><subject>Heterotrophy</subject><subject>Intracellular Space - drug effects</subject><subject>Intracellular Space - metabolism</subject><subject>Lipid</subject><subject>Lipid Metabolism - drug effects</subject><subject>Lipids</subject><subject>Lipids - biosynthesis</subject><subject>Nitrogen - pharmacology</subject><subject>Nutrition</subject><subject>Phosphorus - pharmacology</subject><subject>Photoautotrophy</subject><subject>Phototrophic Processes - drug effects</subject><subject>Phototrophic Processes - genetics</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Starches</subject><subject>Transcription, Genetic - drug effects</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi0Eotstr1D5gsSBbD1x4tg30AooUqVe2rPlOJPGq2wcbC_Vvj0Ou4UjSL80kvV5PJ6PkGtgG2Agbnab1vmQ0A6bkkG1YTmyeUVWIBtelKoRr8mKKcEKWZfVBbmMcccY49CUb8lFWclalcBX5PgwIMW-R5uo7-l0SMEl5yc6m5QwTNSMuZjfRznbYfQBx9HQ-Rhw8q7z0dCn4J_T8JGObnYdzYPF45QGjC4WGTYJO_qEE9IUzBRtcPPS7oq86c0Y8d25rsnj1y8P29vi7v7b9-3nu8JWqkkFAudcYQtNr1gPYGXJLTLZNVKA5LI1raqZQRAdhw6FbKWqlICqb0UrgPE1-XDqOwf_44Ax6b2LdvnDhP4QNdRVxYDXovwPlFeNEiUsqDihNvgYA_Z6Dm5vwlED04shvdMvhvRiSLOc7GZNrs9vHNo9dn-uvSjJwPszYKI1Y593Zl38y2Wfktcyc59OHObl_XQYdLQOJ4udC9mm7rz71yy_AHEUtF0</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Fan, Jianhua</creator><creator>Cui, Yanbin</creator><creator>Zhou, Yang</creator><creator>Wan, Minxi</creator><creator>Wang, Weiliang</creator><creator>Xie, Jingli</creator><creator>Li, Yuanguang</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20140701</creationdate><title>The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription</title><author>Fan, Jianhua ; Cui, Yanbin ; Zhou, Yang ; Wan, Minxi ; Wang, Weiliang ; Xie, Jingli ; Li, Yuanguang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-e13339eb17f90f11c823ce08d7861838bab950ae16d31de68b8949614fb6b6103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological and medical sciences</topic><topic>Biosynthetic Pathways - drug effects</topic><topic>Biosynthetic Pathways - genetics</topic><topic>Chlorella</topic><topic>Chlorella - drug effects</topic><topic>Chlorella - genetics</topic><topic>Chlorella - growth & development</topic><topic>Chlorella - metabolism</topic><topic>Diacylglycerol O-Acyltransferase - genetics</topic><topic>Diacylglycerol O-Acyltransferase - metabolism</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genes</topic><topic>Genetic modification</topic><topic>Heterotrophic Processes - drug effects</topic><topic>Heterotrophic Processes - genetics</topic><topic>Heterotrophy</topic><topic>Intracellular Space - drug effects</topic><topic>Intracellular Space - metabolism</topic><topic>Lipid</topic><topic>Lipid Metabolism - drug effects</topic><topic>Lipids</topic><topic>Lipids - biosynthesis</topic><topic>Nitrogen - pharmacology</topic><topic>Nutrition</topic><topic>Phosphorus - pharmacology</topic><topic>Photoautotrophy</topic><topic>Phototrophic Processes - drug effects</topic><topic>Phototrophic Processes - genetics</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Starches</topic><topic>Transcription, Genetic - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Jianhua</creatorcontrib><creatorcontrib>Cui, Yanbin</creatorcontrib><creatorcontrib>Zhou, Yang</creatorcontrib><creatorcontrib>Wan, Minxi</creatorcontrib><creatorcontrib>Wang, Weiliang</creatorcontrib><creatorcontrib>Xie, Jingli</creatorcontrib><creatorcontrib>Li, Yuanguang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Jianhua</au><au>Cui, Yanbin</au><au>Zhou, Yang</au><au>Wan, Minxi</au><au>Wang, Weiliang</au><au>Xie, Jingli</au><au>Li, Yuanguang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>164</volume><spage>214</spage><epage>220</epage><pages>214-220</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>•Chlorella accumulate considerable lipid during heterotrophic to autotrophic transition.•Lipid and protein contents increased rapidly along with the sharp decline of starch.•Isogenes encoding ME, PEPC and DGAT are related to lipid accumulation.•These genes might be exploited as target genes for genetic modification.•The switch strategy could be developed for efficient algal lipid production.
Heterotrophy to photoautotrophy transition leads to the accumulation of lipids in Chlorella, which has potential to produce both healthy food and biofuels. Therefore, it is of key interest to study the metabolism shift and gene expression changes that influenced by the transition. Both total and neutral lipids contents were increased rapidly within 48h after the switch to light environment, from 24.5% and 18.0% to 35.3% and 27.4%, respectively, along with the sharp decline of starch from 42.3% to 10.4% during 24h photoinduction phase. By analyzing the correlation between lipid content and gene expression, results revealed several genes viz. me g3137, me g6562, pepc g6833, dgat g3280 and dgat g7566, which encode corresponding enzymes in the de novo lipid biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. These results represented the feasibility of lipid production through trophic converting cultivation.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24859213</pmid><doi>10.1016/j.biortech.2014.04.087</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-8524 |
| ispartof | Bioresource technology, 2014-07, Vol.164, p.214-220 |
| issn | 0960-8524 1873-2976 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1544013562 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Biological and medical sciences Biosynthetic Pathways - drug effects Biosynthetic Pathways - genetics Chlorella Chlorella - drug effects Chlorella - genetics Chlorella - growth & development Chlorella - metabolism Diacylglycerol O-Acyltransferase - genetics Diacylglycerol O-Acyltransferase - metabolism Enzymes Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation - drug effects Genes Genetic modification Heterotrophic Processes - drug effects Heterotrophic Processes - genetics Heterotrophy Intracellular Space - drug effects Intracellular Space - metabolism Lipid Lipid Metabolism - drug effects Lipids Lipids - biosynthesis Nitrogen - pharmacology Nutrition Phosphorus - pharmacology Photoautotrophy Phototrophic Processes - drug effects Phototrophic Processes - genetics Real-Time Polymerase Chain Reaction Starches Transcription, Genetic - drug effects |
| title | The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T18%3A20%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effect%20of%20nutrition%20pattern%20alteration%20on%20Chlorella%20pyrenoidosa%20growth,%20lipid%20biosynthesis-related%20gene%20transcription&rft.jtitle=Bioresource%20technology&rft.au=Fan,%20Jianhua&rft.date=2014-07-01&rft.volume=164&rft.spage=214&rft.epage=220&rft.pages=214-220&rft.issn=0960-8524&rft.eissn=1873-2976&rft_id=info:doi/10.1016/j.biortech.2014.04.087&rft_dat=%3Cproquest_cross%3E1534796212%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1534796212&rft_id=info:pmid/24859213&rft_els_id=S0960852414006270&rfr_iscdi=true |