Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum
The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Norther...
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| Veröffentlicht in: | Research in veterinary science 2013-12, Vol.95 (3), p.846-853 |
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| creator | Sadeghinezhad, J. Sorteni, C. Di Guardo, G. D’Agostino, C. Agrimi, U. Nonno, R. Chiocchetti, R. |
| description | The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker.
Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36±12% and 24±5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3±3%, 21±5% and 6±2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR.
These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge. |
| doi_str_mv | 10.1016/j.rvsc.2013.07.028 |
| format | Article |
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Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36±12% and 24±5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3±3%, 21±5% and 6±2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR.
These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2013.07.028</identifier><identifier>PMID: 23969205</identifier><language>eng</language><publisher>England: Elsevier India Pvt Ltd</publisher><subject>Amino acids ; Animals ; Arvicolinae - metabolism ; Arvicolinae - physiology ; Bank vole ; Calbindins - metabolism ; Calcitonin Gene-Related Peptide - metabolism ; Choline O-Acetyltransferase - metabolism ; Enteric nervous system ; Female ; Fluorescent Antibody Technique, Indirect - veterinary ; Immunohistochemistry ; Laboratory animals ; Male ; Microscopy, Fluorescence - veterinary ; Myenteric Plexus - metabolism ; Myenteric Plexus - physiology ; Myodes ; Neurochemical code ; Neurochemistry ; Neurons ; Nitric Oxide Synthase Type I - metabolism ; Peptides ; Prions ; Rodents ; Sheep ; Studies ; Substance P - metabolism ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2013-12, Vol.95 (3), p.846-853</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Jan 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-40d5b1f175a486845db805870219cb191b52389491b9d7b3690e31e07afca9a23</citedby><cites>FETCH-LOGICAL-c450t-40d5b1f175a486845db805870219cb191b52389491b9d7b3690e31e07afca9a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2013.07.028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23969205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sadeghinezhad, J.</creatorcontrib><creatorcontrib>Sorteni, C.</creatorcontrib><creatorcontrib>Di Guardo, G.</creatorcontrib><creatorcontrib>D’Agostino, C.</creatorcontrib><creatorcontrib>Agrimi, U.</creatorcontrib><creatorcontrib>Nonno, R.</creatorcontrib><creatorcontrib>Chiocchetti, R.</creatorcontrib><title>Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker.
Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36±12% and 24±5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3±3%, 21±5% and 6±2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR.
These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Arvicolinae - metabolism</subject><subject>Arvicolinae - physiology</subject><subject>Bank vole</subject><subject>Calbindins - metabolism</subject><subject>Calcitonin Gene-Related Peptide - metabolism</subject><subject>Choline O-Acetyltransferase - metabolism</subject><subject>Enteric nervous system</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect - veterinary</subject><subject>Immunohistochemistry</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Microscopy, Fluorescence - veterinary</subject><subject>Myenteric Plexus - metabolism</subject><subject>Myenteric Plexus - physiology</subject><subject>Myodes</subject><subject>Neurochemical code</subject><subject>Neurochemistry</subject><subject>Neurons</subject><subject>Nitric Oxide Synthase Type I - metabolism</subject><subject>Peptides</subject><subject>Prions</subject><subject>Rodents</subject><subject>Sheep</subject><subject>Studies</subject><subject>Substance P - metabolism</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1v1DAQBmALUdFt4Q9wQJG4lEPCjD8SW-KCKihIbeEAZ8txJpAliRc7WbH_vglbOHBAlSzZkh-PZvwy9hyhQMDy9baI--QLDigKqArg-hHboBI852WJj9kGQMhcca1P2VlKWwCQiNUTdsqFKQ0HtWGfb2mOwX-noUtTPGShzYYDjRPFzme7nn7NKRtXMqb1rnbjj2wfesoubg6hoZR9612k0M_pVdb1NA9P2Unr-kTP7vdz9vX9uy-XH_LrT1cfL99e514qmHIJjaqxxUo5qUstVVNrULoCjsbXaLBWXGgjl4NpqlqUBkggQeVa74zj4pxdHOvuYvg5U5rsMoGnvncjhTlZVFICggD9ACqk5st6AJUlCglQmYW-_IduwxzHZeZVSaNKqWFR_Kh8DClFau0udoOLB4tg1xTt1q4p2jVFC5WF3128uC891wM1f5_8iW0Bb46Alh_edxRt8h2Nnpoukp9sE7r_1b8DDvirXQ</recordid><startdate>201312</startdate><enddate>201312</enddate><creator>Sadeghinezhad, J.</creator><creator>Sorteni, C.</creator><creator>Di Guardo, G.</creator><creator>D’Agostino, C.</creator><creator>Agrimi, U.</creator><creator>Nonno, R.</creator><creator>Chiocchetti, R.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201312</creationdate><title>Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum</title><author>Sadeghinezhad, J. ; Sorteni, C. ; Di Guardo, G. ; D’Agostino, C. ; Agrimi, U. ; Nonno, R. ; Chiocchetti, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-40d5b1f175a486845db805870219cb191b52389491b9d7b3690e31e07afca9a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Arvicolinae - metabolism</topic><topic>Arvicolinae - physiology</topic><topic>Bank vole</topic><topic>Calbindins - metabolism</topic><topic>Calcitonin Gene-Related Peptide - metabolism</topic><topic>Choline O-Acetyltransferase - metabolism</topic><topic>Enteric nervous system</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect - veterinary</topic><topic>Immunohistochemistry</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Microscopy, Fluorescence - veterinary</topic><topic>Myenteric Plexus - metabolism</topic><topic>Myenteric Plexus - physiology</topic><topic>Myodes</topic><topic>Neurochemical code</topic><topic>Neurochemistry</topic><topic>Neurons</topic><topic>Nitric Oxide Synthase Type I - metabolism</topic><topic>Peptides</topic><topic>Prions</topic><topic>Rodents</topic><topic>Sheep</topic><topic>Studies</topic><topic>Substance P - metabolism</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sadeghinezhad, J.</creatorcontrib><creatorcontrib>Sorteni, C.</creatorcontrib><creatorcontrib>Di Guardo, G.</creatorcontrib><creatorcontrib>D’Agostino, C.</creatorcontrib><creatorcontrib>Agrimi, U.</creatorcontrib><creatorcontrib>Nonno, R.</creatorcontrib><creatorcontrib>Chiocchetti, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sadeghinezhad, J.</au><au>Sorteni, C.</au><au>Di Guardo, G.</au><au>D’Agostino, C.</au><au>Agrimi, U.</au><au>Nonno, R.</au><au>Chiocchetti, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2013-12</date><risdate>2013</risdate><volume>95</volume><issue>3</issue><spage>846</spage><epage>853</epage><pages>846-853</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker.
Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36±12% and 24±5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3±3%, 21±5% and 6±2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR.
These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.</abstract><cop>England</cop><pub>Elsevier India Pvt Ltd</pub><pmid>23969205</pmid><doi>10.1016/j.rvsc.2013.07.028</doi><tpages>8</tpages></addata></record> |
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| ispartof | Research in veterinary science, 2013-12, Vol.95 (3), p.846-853 |
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| subjects | Amino acids Animals Arvicolinae - metabolism Arvicolinae - physiology Bank vole Calbindins - metabolism Calcitonin Gene-Related Peptide - metabolism Choline O-Acetyltransferase - metabolism Enteric nervous system Female Fluorescent Antibody Technique, Indirect - veterinary Immunohistochemistry Laboratory animals Male Microscopy, Fluorescence - veterinary Myenteric Plexus - metabolism Myenteric Plexus - physiology Myodes Neurochemical code Neurochemistry Neurons Nitric Oxide Synthase Type I - metabolism Peptides Prions Rodents Sheep Studies Substance P - metabolism Veterinary medicine |
| title | Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum |
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