Investigation into in vitro and in vivo models using intestinal epithelial IPEC‐J2 cells and Caenorhabditis elegans for selecting probiotic candidates to control porcine enterotoxigenic Escherichia coli
AIMS: To identify a fast, economic and reliable method for preselecting lactic acid‐producing bacterial (LAB) isolates to control enterotoxigenic Escherichia coli (ETEC). METHODS AND RESULTS: Two assays with porcine intestinal epithelial IPEC‐J2 cells or Caenorhabditis elegans for selecting effectiv...
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| Veröffentlicht in: | Journal of applied microbiology 2014-07, Vol.117 (1), p.217-226 |
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| creator | Zhou, M Zhu, J Yu, H Yin, X Sabour, P.M Zhao, L Chen, W Gong, J |
| description | AIMS: To identify a fast, economic and reliable method for preselecting lactic acid‐producing bacterial (LAB) isolates to control enterotoxigenic Escherichia coli (ETEC). METHODS AND RESULTS: Two assays with porcine intestinal epithelial IPEC‐J2 cells or Caenorhabditis elegans for selecting effective probiotic candidates were compared. Both assays were based on measuring death of cells or worms caused by ETEC strain JG280. Six of 13 LAB isolates showed ≥50% protection in each assay, among which only four isolates (≥50% protection) were consistently selected by both assays. Isolate CL9 (Lactobacillus reuteri) was further studied. It reduced gene expression of estA, estB and elt in JG280 in both assays. Furthermore, the isolate protected IPEC‐J2 and C. elegans from cell and worm death caused by STa, STb or LT enterotoxin expressed in E. coli DH5α. CL9 also promoted host defensive responses by decreasing IL‐8 and increasing IL‐10 production in IPEC‐J2 cells and expression of antimicrobial peptide genes clec‐60, clec‐85 in C. elegans. CONCLUSIONS: Caenorhabditis elegans is useful for preselecting probiotic candidates to control ETEC after initial screening with IPEC‐J2 cells. SIGNIFICANCE AND IMPACT OF THE STUDY: A combination of IPEC‐J2 cell and C. elegans assays can improve the effectiveness in preselecting probiotic candidates. |
| doi_str_mv | 10.1111/jam.12505 |
| format | Article |
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METHODS AND RESULTS: Two assays with porcine intestinal epithelial IPEC‐J2 cells or Caenorhabditis elegans for selecting effective probiotic candidates were compared. Both assays were based on measuring death of cells or worms caused by ETEC strain JG280. Six of 13 LAB isolates showed ≥50% protection in each assay, among which only four isolates (≥50% protection) were consistently selected by both assays. Isolate CL9 (Lactobacillus reuteri) was further studied. It reduced gene expression of estA, estB and elt in JG280 in both assays. Furthermore, the isolate protected IPEC‐J2 and C. elegans from cell and worm death caused by STa, STb or LT enterotoxin expressed in E. coli DH5α. CL9 also promoted host defensive responses by decreasing IL‐8 and increasing IL‐10 production in IPEC‐J2 cells and expression of antimicrobial peptide genes clec‐60, clec‐85 in C. elegans. CONCLUSIONS: Caenorhabditis elegans is useful for preselecting probiotic candidates to control ETEC after initial screening with IPEC‐J2 cells. SIGNIFICANCE AND IMPACT OF THE STUDY: A combination of IPEC‐J2 cell and C. elegans assays can improve the effectiveness in preselecting probiotic candidates.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12505</identifier><identifier>PMID: 24674595</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford: Published for the Society for Applied Bacteriology by Blackwell Science</publisher><subject>Animals ; Antibiosis ; Antimicrobial Cationic Peptides - agonists ; Antimicrobial Cationic Peptides - biosynthesis ; antimicrobial peptides ; Biological and medical sciences ; Caenorhabditis elegans ; Caenorhabditis elegans - drug effects ; Caenorhabditis elegans - microbiology ; Cell Line ; death ; E coli ; enterotoxigenic Escherichia coli ; Enterotoxigenic Escherichia coli - drug effects ; Enterotoxigenic Escherichia coli - growth & development ; enterotoxins ; Enterotoxins - antagonists & inhibitors ; Enterotoxins - biosynthesis ; Epithelial Cells - drug effects ; Epithelial Cells - microbiology ; Escherichia coli ; Escherichia coli Infections - diet therapy ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - veterinary ; Fundamental and applied biological sciences. Psychology ; Gene expression ; genes ; interleukin-10 ; Interleukin-10 - agonists ; Interleukin-10 - metabolism ; interleukin-8 ; Interleukin-8 - antagonists & inhibitors ; Interleukin-8 - metabolism ; Intestines - drug effects ; Intestines - microbiology ; IPEC‐J2 ; lactic acid‐producing bacteria ; Lactobacillus reuteri ; Lactobacillus reuteri - physiology ; Microbiology ; models for selection ; Models, Biological ; Nematodes ; Probiotics ; Probiotics - pharmacology ; screening ; Swine ; Swine Diseases - diet therapy ; Swine Diseases - microbiology</subject><ispartof>Journal of applied microbiology, 2014-07, Vol.117 (1), p.217-226</ispartof><rights>2014 Society for Applied Microbiology and Her Majesty the Queen in Right of Canada Reproduced with the permission of the Minister of Agriculture and Agri‐Food Canada.</rights><rights>2015 INIST-CNRS</rights><rights>2014 Society for Applied Microbiology and Her Majesty the Queen in Right of Canada Reproduced with the permission of the Minister of Agriculture and Agri-Food Canada.</rights><rights>Copyright © 2014 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4755-580b6f7664e60475f789cd34770643742e8164dbf3e7679f5df2b8767807a2183</citedby><cites>FETCH-LOGICAL-c4755-580b6f7664e60475f789cd34770643742e8164dbf3e7679f5df2b8767807a2183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.12505$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.12505$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28535548$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24674595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, M</creatorcontrib><creatorcontrib>Zhu, J</creatorcontrib><creatorcontrib>Yu, H</creatorcontrib><creatorcontrib>Yin, X</creatorcontrib><creatorcontrib>Sabour, P.M</creatorcontrib><creatorcontrib>Zhao, L</creatorcontrib><creatorcontrib>Chen, W</creatorcontrib><creatorcontrib>Gong, J</creatorcontrib><title>Investigation into in vitro and in vivo models using intestinal epithelial IPEC‐J2 cells and Caenorhabditis elegans for selecting probiotic candidates to control porcine enterotoxigenic Escherichia coli</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>AIMS: To identify a fast, economic and reliable method for preselecting lactic acid‐producing bacterial (LAB) isolates to control enterotoxigenic Escherichia coli (ETEC). METHODS AND RESULTS: Two assays with porcine intestinal epithelial IPEC‐J2 cells or Caenorhabditis elegans for selecting effective probiotic candidates were compared. Both assays were based on measuring death of cells or worms caused by ETEC strain JG280. Six of 13 LAB isolates showed ≥50% protection in each assay, among which only four isolates (≥50% protection) were consistently selected by both assays. Isolate CL9 (Lactobacillus reuteri) was further studied. It reduced gene expression of estA, estB and elt in JG280 in both assays. Furthermore, the isolate protected IPEC‐J2 and C. elegans from cell and worm death caused by STa, STb or LT enterotoxin expressed in E. coli DH5α. CL9 also promoted host defensive responses by decreasing IL‐8 and increasing IL‐10 production in IPEC‐J2 cells and expression of antimicrobial peptide genes clec‐60, clec‐85 in C. elegans. CONCLUSIONS: Caenorhabditis elegans is useful for preselecting probiotic candidates to control ETEC after initial screening with IPEC‐J2 cells. SIGNIFICANCE AND IMPACT OF THE STUDY: A combination of IPEC‐J2 cell and C. elegans assays can improve the effectiveness in preselecting probiotic candidates.</description><subject>Animals</subject><subject>Antibiosis</subject><subject>Antimicrobial Cationic Peptides - agonists</subject><subject>Antimicrobial Cationic Peptides - biosynthesis</subject><subject>antimicrobial peptides</subject><subject>Biological and medical sciences</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - drug effects</subject><subject>Caenorhabditis elegans - microbiology</subject><subject>Cell Line</subject><subject>death</subject><subject>E coli</subject><subject>enterotoxigenic Escherichia coli</subject><subject>Enterotoxigenic Escherichia coli - drug effects</subject><subject>Enterotoxigenic Escherichia coli - growth & development</subject><subject>enterotoxins</subject><subject>Enterotoxins - antagonists & inhibitors</subject><subject>Enterotoxins - biosynthesis</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - microbiology</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - diet therapy</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>genes</subject><subject>interleukin-10</subject><subject>Interleukin-10 - agonists</subject><subject>Interleukin-10 - metabolism</subject><subject>interleukin-8</subject><subject>Interleukin-8 - antagonists & inhibitors</subject><subject>Interleukin-8 - metabolism</subject><subject>Intestines - drug effects</subject><subject>Intestines - microbiology</subject><subject>IPEC‐J2</subject><subject>lactic acid‐producing bacteria</subject><subject>Lactobacillus reuteri</subject><subject>Lactobacillus reuteri - physiology</subject><subject>Microbiology</subject><subject>models for selection</subject><subject>Models, Biological</subject><subject>Nematodes</subject><subject>Probiotics</subject><subject>Probiotics - pharmacology</subject><subject>screening</subject><subject>Swine</subject><subject>Swine Diseases - diet therapy</subject><subject>Swine Diseases - microbiology</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks2O0zAQxyMEYj_gwAuAJYQEh-7ajj_S46oq0NUikGDPkeM46VSp3bWTwt72EXgwnoInYdJ2QUJCwgd7bP3mP-OZybJnjJ4xXOcrsz5jXFL5IDtmuZITrjR_uLPFRFLNj7KTlFaUspxK9Tg74kJpIafyOPux8FuXemhND8ET8H3AjWyhj4EYX-8v20DWoXZdIkMC347Y6ORNR9wG-qXrAM3Fp_ns5933S06s65Ad3WfG-RCXpqqhh0Rc51rjE2lCJAkvth_lNjFUEHqwxKIP1AbVCSZig8c0OrIJ0YJ3xGHYGPrwDVrnkZ4nu3QR7BIMsh08yR41pkvu6eE8za7fzr_M3k-uPr5bzC6uJlZoKSeyoJVqtFLCKYovjS6mts6F1lSJXAvuCqZEXTW500pPG1k3vCrQLKg2nBX5afZ6r4uJ3wxYiXINafyz8S4MqWRSCEqnWOL_QHOlCuzTiL78C12FIWKNd5RUNOeSI_VmT9kYUoquKTcR1ibeloyW4zSUOA3lbhqQfX5QHKq1q3-T9-1H4NUBMMmaronGW0h_uALjSjF--HzPfYXO3f47Ynl58eE-9Iu9R2NCadqIqtefOWUSh1BiA1j-C8EP148</recordid><startdate>201407</startdate><enddate>201407</enddate><creator>Zhou, M</creator><creator>Zhu, J</creator><creator>Yu, H</creator><creator>Yin, X</creator><creator>Sabour, P.M</creator><creator>Zhao, L</creator><creator>Chen, W</creator><creator>Gong, J</creator><general>Published for the Society for Applied Bacteriology by Blackwell Science</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201407</creationdate><title>Investigation into in vitro and in vivo models using intestinal epithelial IPEC‐J2 cells and Caenorhabditis elegans for selecting probiotic candidates to control porcine enterotoxigenic Escherichia coli</title><author>Zhou, M ; Zhu, J ; Yu, H ; Yin, X ; Sabour, P.M ; Zhao, L ; Chen, W ; Gong, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4755-580b6f7664e60475f789cd34770643742e8164dbf3e7679f5df2b8767807a2183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Antibiosis</topic><topic>Antimicrobial Cationic Peptides - agonists</topic><topic>Antimicrobial Cationic Peptides - biosynthesis</topic><topic>antimicrobial peptides</topic><topic>Biological and medical sciences</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - drug effects</topic><topic>Caenorhabditis elegans - microbiology</topic><topic>Cell Line</topic><topic>death</topic><topic>E coli</topic><topic>enterotoxigenic Escherichia coli</topic><topic>Enterotoxigenic Escherichia coli - drug effects</topic><topic>Enterotoxigenic Escherichia coli - growth & development</topic><topic>enterotoxins</topic><topic>Enterotoxins - antagonists & inhibitors</topic><topic>Enterotoxins - biosynthesis</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - microbiology</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - diet therapy</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>genes</topic><topic>interleukin-10</topic><topic>Interleukin-10 - agonists</topic><topic>Interleukin-10 - metabolism</topic><topic>interleukin-8</topic><topic>Interleukin-8 - antagonists & inhibitors</topic><topic>Interleukin-8 - metabolism</topic><topic>Intestines - drug effects</topic><topic>Intestines - microbiology</topic><topic>IPEC‐J2</topic><topic>lactic acid‐producing bacteria</topic><topic>Lactobacillus reuteri</topic><topic>Lactobacillus reuteri - physiology</topic><topic>Microbiology</topic><topic>models for selection</topic><topic>Models, Biological</topic><topic>Nematodes</topic><topic>Probiotics</topic><topic>Probiotics - pharmacology</topic><topic>screening</topic><topic>Swine</topic><topic>Swine Diseases - diet therapy</topic><topic>Swine Diseases - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, M</creatorcontrib><creatorcontrib>Zhu, J</creatorcontrib><creatorcontrib>Yu, H</creatorcontrib><creatorcontrib>Yin, X</creatorcontrib><creatorcontrib>Sabour, P.M</creatorcontrib><creatorcontrib>Zhao, L</creatorcontrib><creatorcontrib>Chen, W</creatorcontrib><creatorcontrib>Gong, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, M</au><au>Zhu, J</au><au>Yu, H</au><au>Yin, X</au><au>Sabour, P.M</au><au>Zhao, L</au><au>Chen, W</au><au>Gong, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation into in vitro and in vivo models using intestinal epithelial IPEC‐J2 cells and Caenorhabditis elegans for selecting probiotic candidates to control porcine enterotoxigenic Escherichia coli</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2014-07</date><risdate>2014</risdate><volume>117</volume><issue>1</issue><spage>217</spage><epage>226</epage><pages>217-226</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>AIMS: To identify a fast, economic and reliable method for preselecting lactic acid‐producing bacterial (LAB) isolates to control enterotoxigenic Escherichia coli (ETEC). METHODS AND RESULTS: Two assays with porcine intestinal epithelial IPEC‐J2 cells or Caenorhabditis elegans for selecting effective probiotic candidates were compared. Both assays were based on measuring death of cells or worms caused by ETEC strain JG280. Six of 13 LAB isolates showed ≥50% protection in each assay, among which only four isolates (≥50% protection) were consistently selected by both assays. Isolate CL9 (Lactobacillus reuteri) was further studied. It reduced gene expression of estA, estB and elt in JG280 in both assays. Furthermore, the isolate protected IPEC‐J2 and C. elegans from cell and worm death caused by STa, STb or LT enterotoxin expressed in E. coli DH5α. CL9 also promoted host defensive responses by decreasing IL‐8 and increasing IL‐10 production in IPEC‐J2 cells and expression of antimicrobial peptide genes clec‐60, clec‐85 in C. elegans. CONCLUSIONS: Caenorhabditis elegans is useful for preselecting probiotic candidates to control ETEC after initial screening with IPEC‐J2 cells. SIGNIFICANCE AND IMPACT OF THE STUDY: A combination of IPEC‐J2 cell and C. elegans assays can improve the effectiveness in preselecting probiotic candidates.</abstract><cop>Oxford</cop><pub>Published for the Society for Applied Bacteriology by Blackwell Science</pub><pmid>24674595</pmid><doi>10.1111/jam.12505</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied microbiology, 2014-07, Vol.117 (1), p.217-226 |
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| subjects | Animals Antibiosis Antimicrobial Cationic Peptides - agonists Antimicrobial Cationic Peptides - biosynthesis antimicrobial peptides Biological and medical sciences Caenorhabditis elegans Caenorhabditis elegans - drug effects Caenorhabditis elegans - microbiology Cell Line death E coli enterotoxigenic Escherichia coli Enterotoxigenic Escherichia coli - drug effects Enterotoxigenic Escherichia coli - growth & development enterotoxins Enterotoxins - antagonists & inhibitors Enterotoxins - biosynthesis Epithelial Cells - drug effects Epithelial Cells - microbiology Escherichia coli Escherichia coli Infections - diet therapy Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Fundamental and applied biological sciences. Psychology Gene expression genes interleukin-10 Interleukin-10 - agonists Interleukin-10 - metabolism interleukin-8 Interleukin-8 - antagonists & inhibitors Interleukin-8 - metabolism Intestines - drug effects Intestines - microbiology IPEC‐J2 lactic acid‐producing bacteria Lactobacillus reuteri Lactobacillus reuteri - physiology Microbiology models for selection Models, Biological Nematodes Probiotics Probiotics - pharmacology screening Swine Swine Diseases - diet therapy Swine Diseases - microbiology |
| title | Investigation into in vitro and in vivo models using intestinal epithelial IPEC‐J2 cells and Caenorhabditis elegans for selecting probiotic candidates to control porcine enterotoxigenic Escherichia coli |
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