Preparation and drug release mechanism of CTS-TAX-NP-MSCs drug delivery system
Targeting delivery of anticancer agents is a promising field in anticancer therapy. Inherent tumor-tropic and migratory properties of mesenchymal stem cells (MSCs) make them potential vehicles for targeting drug delivery systems for tumors. Although, MSCs have been successfully studied and discussed...
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| Veröffentlicht in: | International journal of pharmaceutics 2013-11, Vol.456 (1), p.186-194 |
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| container_title | International journal of pharmaceutics |
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| creator | Dai, Tian Yang, Enyun Sun, Yongjun Zhang, Linan Zhang, Li Shen, Ning Li, Shuo Liu, Lei Xie, Yinghua Wu, Shaomei Gao, Zibin |
| description | Targeting delivery of anticancer agents is a promising field in anticancer therapy. Inherent tumor-tropic and migratory properties of mesenchymal stem cells (MSCs) make them potential vehicles for targeting drug delivery systems for tumors. Although, MSCs have been successfully studied and discussed as a vehicle for cancer gene therapy, they have not yet been studied adequately as a potential vehicle for traditional chemical anticancer drugs. In this study, we have engineered MSCs as a potential targeting delivery vehicle for paclitaxel (TAX)-loaded nanoparticles (NPs). The size, surface charge, starving time of MSCs, incubating time and concentration of NPs could influence the efficiency of NPs uptake. In vitro release of TAX from CTS (chitosan)-TAX-NP-MSCs and the expression of P-glycoprotein demonstrated that release of TAX from MSCs might involve both passive diffusion and active transport. In vitro migration assays indicated that MSCs at passage number 3 have the highest migrating ability. Although, the migration ability of CTS-TAX-NP-MSCs could be inhibited by uptake of CTS-TAX-NPs, this ability could recover 6 days after the internalization. |
| doi_str_mv | 10.1016/j.ijpharm.2013.07.070 |
| format | Article |
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Inherent tumor-tropic and migratory properties of mesenchymal stem cells (MSCs) make them potential vehicles for targeting drug delivery systems for tumors. Although, MSCs have been successfully studied and discussed as a vehicle for cancer gene therapy, they have not yet been studied adequately as a potential vehicle for traditional chemical anticancer drugs. In this study, we have engineered MSCs as a potential targeting delivery vehicle for paclitaxel (TAX)-loaded nanoparticles (NPs). The size, surface charge, starving time of MSCs, incubating time and concentration of NPs could influence the efficiency of NPs uptake. In vitro release of TAX from CTS (chitosan)-TAX-NP-MSCs and the expression of P-glycoprotein demonstrated that release of TAX from MSCs might involve both passive diffusion and active transport. In vitro migration assays indicated that MSCs at passage number 3 have the highest migrating ability. Although, the migration ability of CTS-TAX-NP-MSCs could be inhibited by uptake of CTS-TAX-NPs, this ability could recover 6 days after the internalization.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2013.07.070</identifier><identifier>PMID: 23933442</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>active transport ; Animals ; antineoplastic agents ; Antineoplastic Agents, Phytogenic - administration & dosage ; Antineoplastic Agents, Phytogenic - chemistry ; ATP Binding Cassette Transporter, Sub-Family G, Member 2 ; ATP-Binding Cassette Transporters - metabolism ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; Cancer ; Cell Line, Tumor ; Cell Movement ; chitosan ; Chitosan - chemistry ; Drug Compounding ; Drug delivery ; Drug Delivery Systems ; gene therapy ; Humans ; Lactic Acid - chemistry ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - physiology ; Mice ; migratory behavior ; Multidrug Resistance-Associated Proteins - metabolism ; Nanoparticle ; nanoparticles ; Nanoparticles - chemistry ; neoplasms ; paclitaxel ; Paclitaxel - administration & dosage ; Paclitaxel - chemistry ; Polyglycolic Acid - chemistry ; Rats ; Rats, Sprague-Dawley ; stem cells ; Vehicle</subject><ispartof>International journal of pharmaceutics, 2013-11, Vol.456 (1), p.186-194</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-d6ca2c11e644361ba1813378cb09fb3275a07600bea97c56f64a7c59244538e73</citedby><cites>FETCH-LOGICAL-c558t-d6ca2c11e644361ba1813378cb09fb3275a07600bea97c56f64a7c59244538e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378517313006960$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23933442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dai, Tian</creatorcontrib><creatorcontrib>Yang, Enyun</creatorcontrib><creatorcontrib>Sun, Yongjun</creatorcontrib><creatorcontrib>Zhang, Linan</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Shen, Ning</creatorcontrib><creatorcontrib>Li, Shuo</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Xie, Yinghua</creatorcontrib><creatorcontrib>Wu, Shaomei</creatorcontrib><creatorcontrib>Gao, Zibin</creatorcontrib><title>Preparation and drug release mechanism of CTS-TAX-NP-MSCs drug delivery system</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>Targeting delivery of anticancer agents is a promising field in anticancer therapy. Inherent tumor-tropic and migratory properties of mesenchymal stem cells (MSCs) make them potential vehicles for targeting drug delivery systems for tumors. Although, MSCs have been successfully studied and discussed as a vehicle for cancer gene therapy, they have not yet been studied adequately as a potential vehicle for traditional chemical anticancer drugs. In this study, we have engineered MSCs as a potential targeting delivery vehicle for paclitaxel (TAX)-loaded nanoparticles (NPs). The size, surface charge, starving time of MSCs, incubating time and concentration of NPs could influence the efficiency of NPs uptake. In vitro release of TAX from CTS (chitosan)-TAX-NP-MSCs and the expression of P-glycoprotein demonstrated that release of TAX from MSCs might involve both passive diffusion and active transport. In vitro migration assays indicated that MSCs at passage number 3 have the highest migrating ability. Although, the migration ability of CTS-TAX-NP-MSCs could be inhibited by uptake of CTS-TAX-NPs, this ability could recover 6 days after the internalization.</description><subject>active transport</subject><subject>Animals</subject><subject>antineoplastic agents</subject><subject>Antineoplastic Agents, Phytogenic - administration & dosage</subject><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>ATP Binding Cassette Transporter, Sub-Family G, Member 2</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>Drug Compounding</subject><subject>Drug delivery</subject><subject>Drug Delivery Systems</subject><subject>gene therapy</subject><subject>Humans</subject><subject>Lactic Acid - chemistry</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mice</subject><subject>migratory behavior</subject><subject>Multidrug Resistance-Associated Proteins - metabolism</subject><subject>Nanoparticle</subject><subject>nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>neoplasms</subject><subject>paclitaxel</subject><subject>Paclitaxel - administration & dosage</subject><subject>Paclitaxel - chemistry</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>stem cells</subject><subject>Vehicle</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEQhkVJaZy0P6HtHntZd7Sjj_WpBJMmgTQN2IHehFY7m8jshyOtDf73UVin18DAe3lm5uVh7CuHOQeufm7mfrN9sqGbF8BxDjoNfGAzXmrMUWh1wmaAuswl13jKzmLcAIAqOH5ipwUuEIUoZuzuPtDWBjv6oc9sX2d12D1mgVqykbKO3JPtfeyyocmW61W-vviX393nf1bLOJE1tX5P4ZDFQxyp-8w-NraN9OWY5-zh9-V6eZ3f_r26WV7c5k7Kcsxr5WzhOCclBCpeWV5yTGVdBYumwkJLC1oBVGQX2knVKGFTLgohJJak8Zz9mO5uw_C8oziazkdHbWt7GnbRcCkEgASF76MCUXIhhEqonFAXhhgDNWYbfGfDwXAwr9bNxhytm1frBnQaSHvfji92VUf1_603zQn4PgGNHYx9DD6ah1W6IAF4gSXnifg1EZSs7T0FE52n3lHtA7nR1IN_p8QL7vucYA</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Dai, Tian</creator><creator>Yang, Enyun</creator><creator>Sun, Yongjun</creator><creator>Zhang, Linan</creator><creator>Zhang, Li</creator><creator>Shen, Ning</creator><creator>Li, Shuo</creator><creator>Liu, Lei</creator><creator>Xie, Yinghua</creator><creator>Wu, Shaomei</creator><creator>Gao, Zibin</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20131101</creationdate><title>Preparation and drug release mechanism of CTS-TAX-NP-MSCs drug delivery system</title><author>Dai, Tian ; Yang, Enyun ; Sun, Yongjun ; Zhang, Linan ; Zhang, Li ; Shen, Ning ; Li, Shuo ; Liu, Lei ; Xie, Yinghua ; Wu, Shaomei ; Gao, Zibin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-d6ca2c11e644361ba1813378cb09fb3275a07600bea97c56f64a7c59244538e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>active transport</topic><topic>Animals</topic><topic>antineoplastic agents</topic><topic>Antineoplastic Agents, Phytogenic - administration & dosage</topic><topic>Antineoplastic Agents, Phytogenic - chemistry</topic><topic>ATP Binding Cassette Transporter, Sub-Family G, Member 2</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>Drug Compounding</topic><topic>Drug delivery</topic><topic>Drug Delivery Systems</topic><topic>gene therapy</topic><topic>Humans</topic><topic>Lactic Acid - chemistry</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mice</topic><topic>migratory behavior</topic><topic>Multidrug Resistance-Associated Proteins - metabolism</topic><topic>Nanoparticle</topic><topic>nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>neoplasms</topic><topic>paclitaxel</topic><topic>Paclitaxel - administration & dosage</topic><topic>Paclitaxel - chemistry</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>stem cells</topic><topic>Vehicle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dai, Tian</creatorcontrib><creatorcontrib>Yang, Enyun</creatorcontrib><creatorcontrib>Sun, Yongjun</creatorcontrib><creatorcontrib>Zhang, Linan</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Shen, Ning</creatorcontrib><creatorcontrib>Li, Shuo</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Xie, Yinghua</creatorcontrib><creatorcontrib>Wu, Shaomei</creatorcontrib><creatorcontrib>Gao, Zibin</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dai, Tian</au><au>Yang, Enyun</au><au>Sun, Yongjun</au><au>Zhang, Linan</au><au>Zhang, Li</au><au>Shen, Ning</au><au>Li, Shuo</au><au>Liu, Lei</au><au>Xie, Yinghua</au><au>Wu, Shaomei</au><au>Gao, Zibin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and drug release mechanism of CTS-TAX-NP-MSCs drug delivery system</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>456</volume><issue>1</issue><spage>186</spage><epage>194</epage><pages>186-194</pages><issn>0378-5173</issn><eissn>1873-3476</eissn><abstract>Targeting delivery of anticancer agents is a promising field in anticancer therapy. Inherent tumor-tropic and migratory properties of mesenchymal stem cells (MSCs) make them potential vehicles for targeting drug delivery systems for tumors. Although, MSCs have been successfully studied and discussed as a vehicle for cancer gene therapy, they have not yet been studied adequately as a potential vehicle for traditional chemical anticancer drugs. In this study, we have engineered MSCs as a potential targeting delivery vehicle for paclitaxel (TAX)-loaded nanoparticles (NPs). The size, surface charge, starving time of MSCs, incubating time and concentration of NPs could influence the efficiency of NPs uptake. In vitro release of TAX from CTS (chitosan)-TAX-NP-MSCs and the expression of P-glycoprotein demonstrated that release of TAX from MSCs might involve both passive diffusion and active transport. In vitro migration assays indicated that MSCs at passage number 3 have the highest migrating ability. Although, the migration ability of CTS-TAX-NP-MSCs could be inhibited by uptake of CTS-TAX-NPs, this ability could recover 6 days after the internalization.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23933442</pmid><doi>10.1016/j.ijpharm.2013.07.070</doi><tpages>9</tpages></addata></record> |
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| subjects | active transport Animals antineoplastic agents Antineoplastic Agents, Phytogenic - administration & dosage Antineoplastic Agents, Phytogenic - chemistry ATP Binding Cassette Transporter, Sub-Family G, Member 2 ATP-Binding Cassette Transporters - metabolism ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Cancer Cell Line, Tumor Cell Movement chitosan Chitosan - chemistry Drug Compounding Drug delivery Drug Delivery Systems gene therapy Humans Lactic Acid - chemistry Mesenchymal stem cells Mesenchymal Stromal Cells - physiology Mice migratory behavior Multidrug Resistance-Associated Proteins - metabolism Nanoparticle nanoparticles Nanoparticles - chemistry neoplasms paclitaxel Paclitaxel - administration & dosage Paclitaxel - chemistry Polyglycolic Acid - chemistry Rats Rats, Sprague-Dawley stem cells Vehicle |
| title | Preparation and drug release mechanism of CTS-TAX-NP-MSCs drug delivery system |
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