Gene Ontology based housekeeping gene selection for RNA-seq normalization

•A novel transcriptomic data normalization method based on housekeeping genes.•The housekeeping genes are selected by GO distance and stability analysis.•Normalization results showed the proposed method outperformed traditional approaches.•A web-based online system is available for 12 model species....

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2014-06, Vol.67 (3), p.354-363
Hauptverfasser:	Chen, Chien-Ming, Lu, Yu-Lun, Sio, Chi-Pong, Wu, Guan-Chung, Tzou, Wen-Shyong, Pai, Tun-Wen
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Comparative genomics Differential gene expression Female Fishes - genetics Fishes - physiology Gene Expression Profiling Gene Ontology Genes, Essential Genomics - methods Housekeeping gene Humans Male RNA-seq Sequence Analysis, RNA - methods
Online-Zugang:	Volltext
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