Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey
•C. krusei &K. marxianus as a consortium of mixed culture for SCP from cheese whey.•Cultivation at extreme condition (pH 3.5 & temperature 40°C) to avoid contamination.•Productivity & COD removal was improved in batch process with mixed culture.•Continuous process at 24h HRT to remove CO...
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| Veröffentlicht in: | Bioresource technology 2014-07, Vol.164, p.119-127 |
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| creator | Yadav, J.S.S. Bezawada, J. Ajila, C.M. Yan, S. Tyagi, R.D. Surampalli, R.Y. |
| description | •C. krusei &K. marxianus as a consortium of mixed culture for SCP from cheese whey.•Cultivation at extreme condition (pH 3.5 & temperature 40°C) to avoid contamination.•Productivity & COD removal was improved in batch process with mixed culture.•Continuous process at 24h HRT to remove COD & cultivate SCP, which was rich in lysine.•Productivity & COD removal could be improved by recovering residual soluble protein.
The study was conducted to evaluate the potential of mixed culture of Kluyveromyces marxianus and Candida krusei to enhance COD removal efficiency, minimize contamination at extreme conditions (high temperature 40°C and low pH 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (SCP) using whey as substrate. The batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher COD removal efficacy with 19% higher biomass yield and 33% increased productivity. The maximum COD removal 80.2% (including residual protein) was obtained at 24h HRT with biomass productivity of 0.17g/L/h; however, maximum biomass productivity of 0.38g/L/h and 34% COD removal were obtained at 6h HRT. The results showed that the mixed culture of acid resistance and thermo-tolerant yeasts was a potential way to produce SCP (animal feed) and simultaneous COD removal under extreme operating conditions. |
| doi_str_mv | 10.1016/j.biortech.2014.04.069 |
| format | Article |
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The study was conducted to evaluate the potential of mixed culture of Kluyveromyces marxianus and Candida krusei to enhance COD removal efficiency, minimize contamination at extreme conditions (high temperature 40°C and low pH 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (SCP) using whey as substrate. The batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher COD removal efficacy with 19% higher biomass yield and 33% increased productivity. The maximum COD removal 80.2% (including residual protein) was obtained at 24h HRT with biomass productivity of 0.17g/L/h; however, maximum biomass productivity of 0.38g/L/h and 34% COD removal were obtained at 6h HRT. The results showed that the mixed culture of acid resistance and thermo-tolerant yeasts was a potential way to produce SCP (animal feed) and simultaneous COD removal under extreme operating conditions.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2014.04.069</identifier><identifier>PMID: 24844166</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Amino Acids - analysis ; Batch Cell Culture Techniques - methods ; Biological and medical sciences ; Biological Oxygen Demand Analysis ; Biomass ; Biotechnology ; Candida ; Candida - growth & development ; Candida - metabolism ; Candida krusei ; Cheese whey ; Chemical Precipitation ; Continuous fermentation ; Culture ; Dietary Proteins - metabolism ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Kluyveromyces - growth & development ; Kluyveromyces - metabolism ; Kluyveromyces marxianus ; Lactose - metabolism ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Milk Proteins - chemistry ; Mixed culture ; Organic Chemicals - isolation & purification ; Productivity ; Proteins ; Whey ; Whey Proteins</subject><ispartof>Bioresource technology, 2014-07, Vol.164, p.119-127</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-6d1fc1a4827a6de48f9c1fb6ff9f8e82a9d7da8b2e4405e274871bbc3904c1a53</citedby><cites>FETCH-LOGICAL-c497t-6d1fc1a4827a6de48f9c1fb6ff9f8e82a9d7da8b2e4405e274871bbc3904c1a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852414006099$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28528345$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24844166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yadav, J.S.S.</creatorcontrib><creatorcontrib>Bezawada, J.</creatorcontrib><creatorcontrib>Ajila, C.M.</creatorcontrib><creatorcontrib>Yan, S.</creatorcontrib><creatorcontrib>Tyagi, R.D.</creatorcontrib><creatorcontrib>Surampalli, R.Y.</creatorcontrib><title>Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•C. krusei &K. marxianus as a consortium of mixed culture for SCP from cheese whey.•Cultivation at extreme condition (pH 3.5 & temperature 40°C) to avoid contamination.•Productivity & COD removal was improved in batch process with mixed culture.•Continuous process at 24h HRT to remove COD & cultivate SCP, which was rich in lysine.•Productivity & COD removal could be improved by recovering residual soluble protein.
The study was conducted to evaluate the potential of mixed culture of Kluyveromyces marxianus and Candida krusei to enhance COD removal efficiency, minimize contamination at extreme conditions (high temperature 40°C and low pH 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (SCP) using whey as substrate. The batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher COD removal efficacy with 19% higher biomass yield and 33% increased productivity. The maximum COD removal 80.2% (including residual protein) was obtained at 24h HRT with biomass productivity of 0.17g/L/h; however, maximum biomass productivity of 0.38g/L/h and 34% COD removal were obtained at 6h HRT. The results showed that the mixed culture of acid resistance and thermo-tolerant yeasts was a potential way to produce SCP (animal feed) and simultaneous COD removal under extreme operating conditions.</description><subject>Amino Acids - analysis</subject><subject>Batch Cell Culture Techniques - methods</subject><subject>Biological and medical sciences</subject><subject>Biological Oxygen Demand Analysis</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>Candida</subject><subject>Candida - growth & development</subject><subject>Candida - metabolism</subject><subject>Candida krusei</subject><subject>Cheese whey</subject><subject>Chemical Precipitation</subject><subject>Continuous fermentation</subject><subject>Culture</subject><subject>Dietary Proteins - metabolism</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kluyveromyces - growth & development</subject><subject>Kluyveromyces - metabolism</subject><subject>Kluyveromyces marxianus</subject><subject>Lactose - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Milk Proteins - chemistry</subject><subject>Mixed culture</subject><subject>Organic Chemicals - isolation & purification</subject><subject>Productivity</subject><subject>Proteins</subject><subject>Whey</subject><subject>Whey Proteins</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCX6h8QeKSxXa8_riBVnxUFHGBs-XY49ZLEhc7WbpH_jkOu4UjSCPP5Xk978yL0CUla0qoeLVbdzHlCdztmhHK16SW0I_QiirZNkxL8RitiBakURvGz9B5KTtCSEsle4rOGFecUyFW6OeneA8eu7mf5gw4Bfyxnw97yGk4OCh4sPk-2nEu2I4eb-sTvcXf8lwg4pAyLnG86aFx0Pf4LqcJ4rh0P7sppvG3KuUbO0aH-2Q9zjCkve1xqBPwj1s4PENPgu0LPD_1C_T13dsv2w_N9ef3V9s3143jWk6N8DQ4arli0goPXAXtaOhECDooUMxqL71VHQPOyQaY5ErSrnOtJrzqNu0Fenn8t7r7PkOZzBDLYtuOkOZi6KYKCeFE_AfacqmZ1Kqi4oi6nErJEMxdjvVoB0OJWZIyO_OQlFmSMqSW0FV4eZoxdwP4P7KHaCrw4gTY4mwfsh1dLH-5mqtq-bLX6yMH9Xj7CNkUF2F04GMGNxmf4r-8_AJRDLfJ</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Yadav, J.S.S.</creator><creator>Bezawada, J.</creator><creator>Ajila, C.M.</creator><creator>Yan, S.</creator><creator>Tyagi, R.D.</creator><creator>Surampalli, R.Y.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20140701</creationdate><title>Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey</title><author>Yadav, J.S.S. ; Bezawada, J. ; Ajila, C.M. ; Yan, S. ; Tyagi, R.D. ; Surampalli, R.Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-6d1fc1a4827a6de48f9c1fb6ff9f8e82a9d7da8b2e4405e274871bbc3904c1a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amino Acids - analysis</topic><topic>Batch Cell Culture Techniques - methods</topic><topic>Biological and medical sciences</topic><topic>Biological Oxygen Demand Analysis</topic><topic>Biomass</topic><topic>Biotechnology</topic><topic>Candida</topic><topic>Candida - growth & development</topic><topic>Candida - metabolism</topic><topic>Candida krusei</topic><topic>Cheese whey</topic><topic>Chemical Precipitation</topic><topic>Continuous fermentation</topic><topic>Culture</topic><topic>Dietary Proteins - metabolism</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. 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The study was conducted to evaluate the potential of mixed culture of Kluyveromyces marxianus and Candida krusei to enhance COD removal efficiency, minimize contamination at extreme conditions (high temperature 40°C and low pH 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (SCP) using whey as substrate. The batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher COD removal efficacy with 19% higher biomass yield and 33% increased productivity. The maximum COD removal 80.2% (including residual protein) was obtained at 24h HRT with biomass productivity of 0.17g/L/h; however, maximum biomass productivity of 0.38g/L/h and 34% COD removal were obtained at 6h HRT. The results showed that the mixed culture of acid resistance and thermo-tolerant yeasts was a potential way to produce SCP (animal feed) and simultaneous COD removal under extreme operating conditions.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24844166</pmid><doi>10.1016/j.biortech.2014.04.069</doi><tpages>9</tpages></addata></record> |
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| ispartof | Bioresource technology, 2014-07, Vol.164, p.119-127 |
| issn | 0960-8524 1873-2976 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acids - analysis Batch Cell Culture Techniques - methods Biological and medical sciences Biological Oxygen Demand Analysis Biomass Biotechnology Candida Candida - growth & development Candida - metabolism Candida krusei Cheese whey Chemical Precipitation Continuous fermentation Culture Dietary Proteins - metabolism Fermentation Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Kluyveromyces - growth & development Kluyveromyces - metabolism Kluyveromyces marxianus Lactose - metabolism Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Milk Proteins - chemistry Mixed culture Organic Chemicals - isolation & purification Productivity Proteins Whey Whey Proteins |
| title | Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey |
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