Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils
•High-quality DNA is essential for DNA-based food safety and traceability methods.•A CTAB-based method was optimized and amplifiable DNA yield was improved.•qPCR was used in the development and optimization of a DNA extraction method.•A rapid and economic DNA extraction method was successfully devel...
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Veröffentlicht in: | Food chemistry 2014-09, Vol.158, p.374-383 |
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creator | Ramos-Gómez, Sonia Busto, María D. Perez-Mateos, Manuel Ortega, Natividad |
description | •High-quality DNA is essential for DNA-based food safety and traceability methods.•A CTAB-based method was optimized and amplifiable DNA yield was improved.•qPCR was used in the development and optimization of a DNA extraction method.•A rapid and economic DNA extraction method was successfully developed.•The method applicability was assayed in commercial vegetable oils and margarines.
This study describes the design of a suitable DNA isolation method from commercial vegetable oils for the application of DNA markers for food safety and traceability. Firstly, a comparative study was made of eight methods for the recovery of high quality DNA from olive, sunflower and palm oils, and a CTAB-based method was selected. In order to optimize this method, the effect of the organic compounds and several components in the lysis buffer and the lysis and precipitation time were evaluated. For the purpose of overcoming the limitations detected in spectrophotometric and PCR DNA yield evaluations, the performance of the extraction protocols during the optimization processes was evaluated using qPCR. The suggested DNA extraction optimized is less time consuming than other conventional DNA extraction methods, uses a reduced oil volume and is cheaper than available commercial kits. Additionally, the applicability of this method has been successfully assayed in ten commercial vegetable oils and derivatives. |
doi_str_mv | 10.1016/j.foodchem.2014.02.142 |
format | Article |
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This study describes the design of a suitable DNA isolation method from commercial vegetable oils for the application of DNA markers for food safety and traceability. Firstly, a comparative study was made of eight methods for the recovery of high quality DNA from olive, sunflower and palm oils, and a CTAB-based method was selected. In order to optimize this method, the effect of the organic compounds and several components in the lysis buffer and the lysis and precipitation time were evaluated. For the purpose of overcoming the limitations detected in spectrophotometric and PCR DNA yield evaluations, the performance of the extraction protocols during the optimization processes was evaluated using qPCR. The suggested DNA extraction optimized is less time consuming than other conventional DNA extraction methods, uses a reduced oil volume and is cheaper than available commercial kits. Additionally, the applicability of this method has been successfully assayed in ten commercial vegetable oils and derivatives.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2014.02.142</identifier><identifier>PMID: 24731357</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Commercial vegetable oil ; CTAB ; DNA detection ; DNA extraction ; Food toxicology ; Helianthus ; Medical sciences ; Nucleic Acid Amplification Techniques ; Olea ; Plant Oils - analysis ; qPCR ; Real-Time Polymerase Chain Reaction - methods ; Toxicology</subject><ispartof>Food chemistry, 2014-09, Vol.158, p.374-383</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-31dc3a907455c10c9b275eece6926dd6457d695e2d970e1e0e0e2022ac9152693</citedby><cites>FETCH-LOGICAL-c431t-31dc3a907455c10c9b275eece6926dd6457d695e2d970e1e0e0e2022ac9152693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodchem.2014.02.142$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28416264$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24731357$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramos-Gómez, Sonia</creatorcontrib><creatorcontrib>Busto, María D.</creatorcontrib><creatorcontrib>Perez-Mateos, Manuel</creatorcontrib><creatorcontrib>Ortega, Natividad</creatorcontrib><title>Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•High-quality DNA is essential for DNA-based food safety and traceability methods.•A CTAB-based method was optimized and amplifiable DNA yield was improved.•qPCR was used in the development and optimization of a DNA extraction method.•A rapid and economic DNA extraction method was successfully developed.•The method applicability was assayed in commercial vegetable oils and margarines.
This study describes the design of a suitable DNA isolation method from commercial vegetable oils for the application of DNA markers for food safety and traceability. Firstly, a comparative study was made of eight methods for the recovery of high quality DNA from olive, sunflower and palm oils, and a CTAB-based method was selected. In order to optimize this method, the effect of the organic compounds and several components in the lysis buffer and the lysis and precipitation time were evaluated. For the purpose of overcoming the limitations detected in spectrophotometric and PCR DNA yield evaluations, the performance of the extraction protocols during the optimization processes was evaluated using qPCR. The suggested DNA extraction optimized is less time consuming than other conventional DNA extraction methods, uses a reduced oil volume and is cheaper than available commercial kits. Additionally, the applicability of this method has been successfully assayed in ten commercial vegetable oils and derivatives.</description><subject>Biological and medical sciences</subject><subject>Commercial vegetable oil</subject><subject>CTAB</subject><subject>DNA detection</subject><subject>DNA extraction</subject><subject>Food toxicology</subject><subject>Helianthus</subject><subject>Medical sciences</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Olea</subject><subject>Plant Oils - analysis</subject><subject>qPCR</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Toxicology</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1vEzEQhi1ERdPCX6h8QeKyi7_WXt-o0kKRKlpVcLYce5Y6stfB3kTKv2ejpHAEzWEuzzszmgehK0paSqj8uG6HnL17htQyQkVLWEsFe4UWtFe8UUSx12hBOOmbngp5ji5qXRNCZrZ_g86ZUJzyTi1QuIEdxLxJME44D9jiBNNz9njKuIDLOyh7bEePbdrEMARnp5BHfPPtGq_2M2FjM4UE-HH5hIeSE3Y5JSgu2Ih38BMmu4qAc4j1LTobbKzw7tQv0Y_Pt9-Xd839w5evy-v7xglOp4ZT77jVRImuc5Q4vWKqA3AgNZPeS9EpL3UHzGtFgAKZixHGrNO0Y1LzS_ThOHdT8q8t1MmkUB3EaEfI22poJ7jWmmn2HyiVivGeiBmVR9SVXGuBwWxKSLbsDSXmYMSszYsRczBiCDOzkTl4ddqxXSXwf2IvCmbg_Qmw1dk4FDu6UP9yvaCSycMFn44czM_bBSimugCjAx9mUZPxOfzrlt-Eb6vD</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Ramos-Gómez, Sonia</creator><creator>Busto, María D.</creator><creator>Perez-Mateos, Manuel</creator><creator>Ortega, Natividad</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20140901</creationdate><title>Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils</title><author>Ramos-Gómez, Sonia ; Busto, María D. ; Perez-Mateos, Manuel ; Ortega, Natividad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-31dc3a907455c10c9b275eece6926dd6457d695e2d970e1e0e0e2022ac9152693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological and medical sciences</topic><topic>Commercial vegetable oil</topic><topic>CTAB</topic><topic>DNA detection</topic><topic>DNA extraction</topic><topic>Food toxicology</topic><topic>Helianthus</topic><topic>Medical sciences</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Olea</topic><topic>Plant Oils - analysis</topic><topic>qPCR</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramos-Gómez, Sonia</creatorcontrib><creatorcontrib>Busto, María D.</creatorcontrib><creatorcontrib>Perez-Mateos, Manuel</creatorcontrib><creatorcontrib>Ortega, Natividad</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramos-Gómez, Sonia</au><au>Busto, María D.</au><au>Perez-Mateos, Manuel</au><au>Ortega, Natividad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>158</volume><spage>374</spage><epage>383</epage><pages>374-383</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>•High-quality DNA is essential for DNA-based food safety and traceability methods.•A CTAB-based method was optimized and amplifiable DNA yield was improved.•qPCR was used in the development and optimization of a DNA extraction method.•A rapid and economic DNA extraction method was successfully developed.•The method applicability was assayed in commercial vegetable oils and margarines.
This study describes the design of a suitable DNA isolation method from commercial vegetable oils for the application of DNA markers for food safety and traceability. Firstly, a comparative study was made of eight methods for the recovery of high quality DNA from olive, sunflower and palm oils, and a CTAB-based method was selected. In order to optimize this method, the effect of the organic compounds and several components in the lysis buffer and the lysis and precipitation time were evaluated. For the purpose of overcoming the limitations detected in spectrophotometric and PCR DNA yield evaluations, the performance of the extraction protocols during the optimization processes was evaluated using qPCR. The suggested DNA extraction optimized is less time consuming than other conventional DNA extraction methods, uses a reduced oil volume and is cheaper than available commercial kits. Additionally, the applicability of this method has been successfully assayed in ten commercial vegetable oils and derivatives.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24731357</pmid><doi>10.1016/j.foodchem.2014.02.142</doi><tpages>10</tpages></addata></record> |
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subjects | Biological and medical sciences Commercial vegetable oil CTAB DNA detection DNA extraction Food toxicology Helianthus Medical sciences Nucleic Acid Amplification Techniques Olea Plant Oils - analysis qPCR Real-Time Polymerase Chain Reaction - methods Toxicology |
title | Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils |
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