Optimization of SELEX: Comparison of different methods for monitoring the progress of in vitro selection of aptamers

•Aptamer selection is often hindered due to accompanying adverse effects.•Assays are described for measuring aptamer pool complexity and binding affinity.•Methods are compared using anti-streptavidin aptamer selection as model. Oligonucleotide aptamers are selected from libraries typically comprisin...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 2014-03, Vol.91, p.151-159
Hauptverfasser:	Mencin, Nina, Šmuc, Tina, Vraničar, Marko, Mavri, Jan, Hren, Matjaž, Galeša, Katja, Krkoč, Peter, Ulrich, Henning, Šolar, Borut
Format:	Artikel
Sprache:	eng
Schlagworte:	Aptamer Aptamer pool evolution Aptamers, Nucleotide - chemistry Evolution monitoring Oligonucleotides - chemistry Selection progress SELEX SELEX Aptamer Technique - methods Streptavidin - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!