The effectiveness of tetanus toxin on sciatic nerve regeneration: A preliminary experimental study in rats
Object The purpose was to investigate the effects of local tetanus toxin (TeTx) application on sciatic nerve regeneration following a rat model of transection injury. Methods After both sciatic nerves were transected and repaired with three epineural sutures, 12 male Wistar albino rats were divided...
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| Veröffentlicht in: | Microsurgery 2014-07, Vol.34 (5), p.384-389 |
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| container_title | Microsurgery |
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| creator | Gunay, Huseyin Kucuk, Levent Erbas, Oytun Atamaz, Funda Calis Kucuk, Ulku Coskunol, Erhan |
| description | Object
The purpose was to investigate the effects of local tetanus toxin (TeTx) application on sciatic nerve regeneration following a rat model of transection injury.
Methods
After both sciatic nerves were transected and repaired with three epineural sutures, 12 male Wistar albino rats were divided into two groups. 0.25 ml (2.5 flocculation units) TeTx was injected into a piece of absorbable gelatin sponge in TeTx group. In controls, 0.25 ml saline injected. Assessments were performed by using climbing degrees, compound muscle action potentials (CMAPs) and histological parameters (axon number and axonal diameter) 12th week.
Results
CMAPs amplitudes were 11.6 ± 4.7 mV and 1.4 ± 1.3 mV in gastrocnemius and interdigital muscles in TeTx group (5.8 ± 2.4 mV and 0.2 ± 0.1 mV, P < 0.05). Climbing degrees were significantly different (61.6 ± 1.7 vs. 38.3 ± 2.6, P |
| doi_str_mv | 10.1002/micr.22249 |
| format | Article |
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The purpose was to investigate the effects of local tetanus toxin (TeTx) application on sciatic nerve regeneration following a rat model of transection injury.
Methods
After both sciatic nerves were transected and repaired with three epineural sutures, 12 male Wistar albino rats were divided into two groups. 0.25 ml (2.5 flocculation units) TeTx was injected into a piece of absorbable gelatin sponge in TeTx group. In controls, 0.25 ml saline injected. Assessments were performed by using climbing degrees, compound muscle action potentials (CMAPs) and histological parameters (axon number and axonal diameter) 12th week.
Results
CMAPs amplitudes were 11.6 ± 4.7 mV and 1.4 ± 1.3 mV in gastrocnemius and interdigital muscles in TeTx group (5.8 ± 2.4 mV and 0.2 ± 0.1 mV, P < 0.05). Climbing degrees were significantly different (61.6 ± 1.7 vs. 38.3 ± 2.6, P < 0.05). Total axon numbers were higher (1341.1 ± 57.3 vs. 877.5 ± 34.9, P < 0.05) and the mean axon diameter was smaller (4.2 ± 2.1 vs. 2.5 ± 1.9, P < 0.05) in the TeTx group.
Conclusion
This preliminary study firstly demonstrated the effectiveness of TeTx on nerve repair in experimental sciatic rat model based on functional, electromyographic and histological parameters. © 2014 Wiley Periodicals, Inc. Microsurgery 34:384–389, 2014.</description><identifier>ISSN: 0738-1085</identifier><identifier>EISSN: 1098-2752</identifier><identifier>DOI: 10.1002/micr.22249</identifier><identifier>PMID: 24665036</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; Electromyography ; Male ; Models, Animal ; Nerve Regeneration - drug effects ; Neurotoxins - pharmacology ; Rats, Wistar ; Tetanus Toxin - pharmacology</subject><ispartof>Microsurgery, 2014-07, Vol.34 (5), p.384-389</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4969-4e3254033da883b9ac9bca510e105215003654e142366802d5cdec7d1464bafe3</citedby><orcidid>0000-0003-1122-4459</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmicr.22249$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmicr.22249$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24665036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gunay, Huseyin</creatorcontrib><creatorcontrib>Kucuk, Levent</creatorcontrib><creatorcontrib>Erbas, Oytun</creatorcontrib><creatorcontrib>Atamaz, Funda Calis</creatorcontrib><creatorcontrib>Kucuk, Ulku</creatorcontrib><creatorcontrib>Coskunol, Erhan</creatorcontrib><title>The effectiveness of tetanus toxin on sciatic nerve regeneration: A preliminary experimental study in rats</title><title>Microsurgery</title><addtitle>Microsurgery</addtitle><description>Object
The purpose was to investigate the effects of local tetanus toxin (TeTx) application on sciatic nerve regeneration following a rat model of transection injury.
Methods
After both sciatic nerves were transected and repaired with three epineural sutures, 12 male Wistar albino rats were divided into two groups. 0.25 ml (2.5 flocculation units) TeTx was injected into a piece of absorbable gelatin sponge in TeTx group. In controls, 0.25 ml saline injected. Assessments were performed by using climbing degrees, compound muscle action potentials (CMAPs) and histological parameters (axon number and axonal diameter) 12th week.
Results
CMAPs amplitudes were 11.6 ± 4.7 mV and 1.4 ± 1.3 mV in gastrocnemius and interdigital muscles in TeTx group (5.8 ± 2.4 mV and 0.2 ± 0.1 mV, P < 0.05). Climbing degrees were significantly different (61.6 ± 1.7 vs. 38.3 ± 2.6, P < 0.05). Total axon numbers were higher (1341.1 ± 57.3 vs. 877.5 ± 34.9, P < 0.05) and the mean axon diameter was smaller (4.2 ± 2.1 vs. 2.5 ± 1.9, P < 0.05) in the TeTx group.
Conclusion
This preliminary study firstly demonstrated the effectiveness of TeTx on nerve repair in experimental sciatic rat model based on functional, electromyographic and histological parameters. © 2014 Wiley Periodicals, Inc. Microsurgery 34:384–389, 2014.</description><subject>Animals</subject><subject>Electromyography</subject><subject>Male</subject><subject>Models, Animal</subject><subject>Nerve Regeneration - drug effects</subject><subject>Neurotoxins - pharmacology</subject><subject>Rats, Wistar</subject><subject>Tetanus Toxin - pharmacology</subject><issn>0738-1085</issn><issn>1098-2752</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1PGzEQhq2qCALl0h9QWeqllwV_7q57QxGESAGkCsTRcryzrdNdb2p7afLvcQhw4DSjmecdzcyL0FdKzigh7Lx3NpwxxoT6hCaUqLpglWSf0YRUvC4oqeUROo5xRQhRqlKH6IiJspSElxO0uv8DGNoWbHJP4CFGPLQ4QTJ-jDgNG-fx4HG0ziRnsYfwBDjA74yGXBn8T3yB1wE61ztvwhbDZg3B9eCT6XBMY7PFeURm4xd00JouwulrPEEPV5f30-ticTebTy8WhRWqVIUAzqQgnDemrvlSGauW1khKgBLJqCR5bymACsbLsiaskbYBWzVUlGJpWuAn6Md-7joM_0aISfcuWug642EYo6ZScFZLJURGv39AV8MYfN5uRzGpJFc0U99eqXHZQ6PX-b58qn77YgboHvjvOti-9ynRO3_0zh_94o--mU9_vWRZU-w1LibYvGtM-KvLildSP97O9Gw6u7pW9a3m_Bl-bJG_</recordid><startdate>201407</startdate><enddate>201407</enddate><creator>Gunay, Huseyin</creator><creator>Kucuk, Levent</creator><creator>Erbas, Oytun</creator><creator>Atamaz, Funda Calis</creator><creator>Kucuk, Ulku</creator><creator>Coskunol, Erhan</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1122-4459</orcidid></search><sort><creationdate>201407</creationdate><title>The effectiveness of tetanus toxin on sciatic nerve regeneration: A preliminary experimental study in rats</title><author>Gunay, Huseyin ; Kucuk, Levent ; Erbas, Oytun ; Atamaz, Funda Calis ; Kucuk, Ulku ; Coskunol, Erhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4969-4e3254033da883b9ac9bca510e105215003654e142366802d5cdec7d1464bafe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Electromyography</topic><topic>Male</topic><topic>Models, Animal</topic><topic>Nerve Regeneration - drug effects</topic><topic>Neurotoxins - pharmacology</topic><topic>Rats, Wistar</topic><topic>Tetanus Toxin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gunay, Huseyin</creatorcontrib><creatorcontrib>Kucuk, Levent</creatorcontrib><creatorcontrib>Erbas, Oytun</creatorcontrib><creatorcontrib>Atamaz, Funda Calis</creatorcontrib><creatorcontrib>Kucuk, Ulku</creatorcontrib><creatorcontrib>Coskunol, Erhan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microsurgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gunay, Huseyin</au><au>Kucuk, Levent</au><au>Erbas, Oytun</au><au>Atamaz, Funda Calis</au><au>Kucuk, Ulku</au><au>Coskunol, Erhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effectiveness of tetanus toxin on sciatic nerve regeneration: A preliminary experimental study in rats</atitle><jtitle>Microsurgery</jtitle><addtitle>Microsurgery</addtitle><date>2014-07</date><risdate>2014</risdate><volume>34</volume><issue>5</issue><spage>384</spage><epage>389</epage><pages>384-389</pages><issn>0738-1085</issn><eissn>1098-2752</eissn><abstract>Object
The purpose was to investigate the effects of local tetanus toxin (TeTx) application on sciatic nerve regeneration following a rat model of transection injury.
Methods
After both sciatic nerves were transected and repaired with three epineural sutures, 12 male Wistar albino rats were divided into two groups. 0.25 ml (2.5 flocculation units) TeTx was injected into a piece of absorbable gelatin sponge in TeTx group. In controls, 0.25 ml saline injected. Assessments were performed by using climbing degrees, compound muscle action potentials (CMAPs) and histological parameters (axon number and axonal diameter) 12th week.
Results
CMAPs amplitudes were 11.6 ± 4.7 mV and 1.4 ± 1.3 mV in gastrocnemius and interdigital muscles in TeTx group (5.8 ± 2.4 mV and 0.2 ± 0.1 mV, P < 0.05). Climbing degrees were significantly different (61.6 ± 1.7 vs. 38.3 ± 2.6, P < 0.05). Total axon numbers were higher (1341.1 ± 57.3 vs. 877.5 ± 34.9, P < 0.05) and the mean axon diameter was smaller (4.2 ± 2.1 vs. 2.5 ± 1.9, P < 0.05) in the TeTx group.
Conclusion
This preliminary study firstly demonstrated the effectiveness of TeTx on nerve repair in experimental sciatic rat model based on functional, electromyographic and histological parameters. © 2014 Wiley Periodicals, Inc. Microsurgery 34:384–389, 2014.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24665036</pmid><doi>10.1002/micr.22249</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-1122-4459</orcidid></addata></record> |
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| source | MEDLINE; Wiley Journals |
| subjects | Animals Electromyography Male Models, Animal Nerve Regeneration - drug effects Neurotoxins - pharmacology Rats, Wistar Tetanus Toxin - pharmacology |
| title | The effectiveness of tetanus toxin on sciatic nerve regeneration: A preliminary experimental study in rats |
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