Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis
Background The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflam...
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| Veröffentlicht in: | Surgical endoscopy 2014-08, Vol.28 (8), p.2437-2442 |
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| creator | Krebs, Thomas Boettcher, Michael Schäfer, Hansjörg Eschenburg, Georg Wenke, Katharina Appl, Birgit Roth, Beate Andreas, Thomas Schmitz, Carla Fahje, Rebecca Jacobsen, Birthe Tiemann, Bastian Reinshagen, Konrad Hecher, Kurt Bergholz, Robert |
| description | Background
The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.
Methods
Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.
Results
In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.
Conclusions
Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation. |
| doi_str_mv | 10.1007/s00464-014-3494-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1542296311</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3357617901</sourcerecordid><originalsourceid>FETCH-LOGICAL-c442t-dd62a5a1fd0c62eafe1d94e53bea7adc31491ce5229ddfce1d6e0291ba1455283</originalsourceid><addsrcrecordid>eNp1kUFP4zAQhS20CLqFH8BlZWkvXAIex06bI6qARULiAmfLtccQlMTFTlD59zuhsEJIe7Ls971njx9jJyDOQIjFeRZCVaoQoIpS1arY7rEZqFIWUsLyB5uJuhSFXNTqkP3M-VkQXoM-YIeSbEsQixlrrseBN31obdfZoYk9t73nuN0kzHnaxsBvVitCuOUBB9tyQte8ix7bSaSzmF3cNI6YAdMr9u8xISb-aPOQSH1qcpOP2H6wbcbjj3XOHq4u71d_itu765vVxW3hlJJD4X0lrbYQvHCVRBsQfK1Ql2u0C-tdCTSDQy1l7X1wpFYoZA1rC0pruSzn7HSXu0nxZcQ8mK7JDtvW9hjHbEAr8lYlAKG_v6HPcUw9vW6ioFoqoTVRsKMczZITBrNJTWfTmwFhph7MrgdDPZipB7Mlz6-P5HHdof_n-Px4AuQOyCT1j5i-XP3f1L9pT5SK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1541684055</pqid></control><display><type>article</type><title>Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Krebs, Thomas ; Boettcher, Michael ; Schäfer, Hansjörg ; Eschenburg, Georg ; Wenke, Katharina ; Appl, Birgit ; Roth, Beate ; Andreas, Thomas ; Schmitz, Carla ; Fahje, Rebecca ; Jacobsen, Birthe ; Tiemann, Bastian ; Reinshagen, Konrad ; Hecher, Kurt ; Bergholz, Robert</creator><creatorcontrib>Krebs, Thomas ; Boettcher, Michael ; Schäfer, Hansjörg ; Eschenburg, Georg ; Wenke, Katharina ; Appl, Birgit ; Roth, Beate ; Andreas, Thomas ; Schmitz, Carla ; Fahje, Rebecca ; Jacobsen, Birthe ; Tiemann, Bastian ; Reinshagen, Konrad ; Hecher, Kurt ; Bergholz, Robert</creatorcontrib><description>Background
The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.
Methods
Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.
Results
In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.
Conclusions
Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation.</description><identifier>ISSN: 0930-2794</identifier><identifier>EISSN: 1432-2218</identifier><identifier>DOI: 10.1007/s00464-014-3494-x</identifier><identifier>PMID: 24648107</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Abdomen ; Abdominal Surgery ; Animals ; Cell Count ; Cesarean section ; Disease Models, Animal ; Female ; Fetoscopy ; Fetuses ; Gastroenterology ; Gastroschisis - pathology ; Gastroschisis - surgery ; Gynecology ; Hepatology ; Immunohistochemistry ; Inflammation - metabolism ; Inflammation - pathology ; Interstitial Cells of Cajal - metabolism ; Intervention ; Intestinal Mucosa - pathology ; Medicine ; Medicine & Public Health ; Mortality ; Motility ; Pregnancy ; Proctology ; Sheep ; Smooth muscle ; Surgery</subject><ispartof>Surgical endoscopy, 2014-08, Vol.28 (8), p.2437-2442</ispartof><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-dd62a5a1fd0c62eafe1d94e53bea7adc31491ce5229ddfce1d6e0291ba1455283</citedby><cites>FETCH-LOGICAL-c442t-dd62a5a1fd0c62eafe1d94e53bea7adc31491ce5229ddfce1d6e0291ba1455283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00464-014-3494-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00464-014-3494-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24648107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krebs, Thomas</creatorcontrib><creatorcontrib>Boettcher, Michael</creatorcontrib><creatorcontrib>Schäfer, Hansjörg</creatorcontrib><creatorcontrib>Eschenburg, Georg</creatorcontrib><creatorcontrib>Wenke, Katharina</creatorcontrib><creatorcontrib>Appl, Birgit</creatorcontrib><creatorcontrib>Roth, Beate</creatorcontrib><creatorcontrib>Andreas, Thomas</creatorcontrib><creatorcontrib>Schmitz, Carla</creatorcontrib><creatorcontrib>Fahje, Rebecca</creatorcontrib><creatorcontrib>Jacobsen, Birthe</creatorcontrib><creatorcontrib>Tiemann, Bastian</creatorcontrib><creatorcontrib>Reinshagen, Konrad</creatorcontrib><creatorcontrib>Hecher, Kurt</creatorcontrib><creatorcontrib>Bergholz, Robert</creatorcontrib><title>Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis</title><title>Surgical endoscopy</title><addtitle>Surg Endosc</addtitle><addtitle>Surg Endosc</addtitle><description>Background
The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.
Methods
Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.
Results
In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.
Conclusions
Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation.</description><subject>Abdomen</subject><subject>Abdominal Surgery</subject><subject>Animals</subject><subject>Cell Count</subject><subject>Cesarean section</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Fetoscopy</subject><subject>Fetuses</subject><subject>Gastroenterology</subject><subject>Gastroschisis - pathology</subject><subject>Gastroschisis - surgery</subject><subject>Gynecology</subject><subject>Hepatology</subject><subject>Immunohistochemistry</subject><subject>Inflammation - metabolism</subject><subject>Inflammation - pathology</subject><subject>Interstitial Cells of Cajal - metabolism</subject><subject>Intervention</subject><subject>Intestinal Mucosa - pathology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mortality</subject><subject>Motility</subject><subject>Pregnancy</subject><subject>Proctology</subject><subject>Sheep</subject><subject>Smooth muscle</subject><subject>Surgery</subject><issn>0930-2794</issn><issn>1432-2218</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kUFP4zAQhS20CLqFH8BlZWkvXAIex06bI6qARULiAmfLtccQlMTFTlD59zuhsEJIe7Ls971njx9jJyDOQIjFeRZCVaoQoIpS1arY7rEZqFIWUsLyB5uJuhSFXNTqkP3M-VkQXoM-YIeSbEsQixlrrseBN31obdfZoYk9t73nuN0kzHnaxsBvVitCuOUBB9tyQte8ix7bSaSzmF3cNI6YAdMr9u8xISb-aPOQSH1qcpOP2H6wbcbjj3XOHq4u71d_itu765vVxW3hlJJD4X0lrbYQvHCVRBsQfK1Ql2u0C-tdCTSDQy1l7X1wpFYoZA1rC0pruSzn7HSXu0nxZcQ8mK7JDtvW9hjHbEAr8lYlAKG_v6HPcUw9vW6ioFoqoTVRsKMczZITBrNJTWfTmwFhph7MrgdDPZipB7Mlz6-P5HHdof_n-Px4AuQOyCT1j5i-XP3f1L9pT5SK</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Krebs, Thomas</creator><creator>Boettcher, Michael</creator><creator>Schäfer, Hansjörg</creator><creator>Eschenburg, Georg</creator><creator>Wenke, Katharina</creator><creator>Appl, Birgit</creator><creator>Roth, Beate</creator><creator>Andreas, Thomas</creator><creator>Schmitz, Carla</creator><creator>Fahje, Rebecca</creator><creator>Jacobsen, Birthe</creator><creator>Tiemann, Bastian</creator><creator>Reinshagen, Konrad</creator><creator>Hecher, Kurt</creator><creator>Bergholz, Robert</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20140801</creationdate><title>Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis</title><author>Krebs, Thomas ; Boettcher, Michael ; Schäfer, Hansjörg ; Eschenburg, Georg ; Wenke, Katharina ; Appl, Birgit ; Roth, Beate ; Andreas, Thomas ; Schmitz, Carla ; Fahje, Rebecca ; Jacobsen, Birthe ; Tiemann, Bastian ; Reinshagen, Konrad ; Hecher, Kurt ; Bergholz, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-dd62a5a1fd0c62eafe1d94e53bea7adc31491ce5229ddfce1d6e0291ba1455283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Abdomen</topic><topic>Abdominal Surgery</topic><topic>Animals</topic><topic>Cell Count</topic><topic>Cesarean section</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Fetoscopy</topic><topic>Fetuses</topic><topic>Gastroenterology</topic><topic>Gastroschisis - pathology</topic><topic>Gastroschisis - surgery</topic><topic>Gynecology</topic><topic>Hepatology</topic><topic>Immunohistochemistry</topic><topic>Inflammation - metabolism</topic><topic>Inflammation - pathology</topic><topic>Interstitial Cells of Cajal - metabolism</topic><topic>Intervention</topic><topic>Intestinal Mucosa - pathology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mortality</topic><topic>Motility</topic><topic>Pregnancy</topic><topic>Proctology</topic><topic>Sheep</topic><topic>Smooth muscle</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krebs, Thomas</creatorcontrib><creatorcontrib>Boettcher, Michael</creatorcontrib><creatorcontrib>Schäfer, Hansjörg</creatorcontrib><creatorcontrib>Eschenburg, Georg</creatorcontrib><creatorcontrib>Wenke, Katharina</creatorcontrib><creatorcontrib>Appl, Birgit</creatorcontrib><creatorcontrib>Roth, Beate</creatorcontrib><creatorcontrib>Andreas, Thomas</creatorcontrib><creatorcontrib>Schmitz, Carla</creatorcontrib><creatorcontrib>Fahje, Rebecca</creatorcontrib><creatorcontrib>Jacobsen, Birthe</creatorcontrib><creatorcontrib>Tiemann, Bastian</creatorcontrib><creatorcontrib>Reinshagen, Konrad</creatorcontrib><creatorcontrib>Hecher, Kurt</creatorcontrib><creatorcontrib>Bergholz, Robert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Surgical endoscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krebs, Thomas</au><au>Boettcher, Michael</au><au>Schäfer, Hansjörg</au><au>Eschenburg, Georg</au><au>Wenke, Katharina</au><au>Appl, Birgit</au><au>Roth, Beate</au><au>Andreas, Thomas</au><au>Schmitz, Carla</au><au>Fahje, Rebecca</au><au>Jacobsen, Birthe</au><au>Tiemann, Bastian</au><au>Reinshagen, Konrad</au><au>Hecher, Kurt</au><au>Bergholz, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis</atitle><jtitle>Surgical endoscopy</jtitle><stitle>Surg Endosc</stitle><addtitle>Surg Endosc</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>28</volume><issue>8</issue><spage>2437</spage><epage>2442</epage><pages>2437-2442</pages><issn>0930-2794</issn><eissn>1432-2218</eissn><abstract>Background
The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.
Methods
Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.
Results
In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.
Conclusions
Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24648107</pmid><doi>10.1007/s00464-014-3494-x</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Abdomen Abdominal Surgery Animals Cell Count Cesarean section Disease Models, Animal Female Fetoscopy Fetuses Gastroenterology Gastroschisis - pathology Gastroschisis - surgery Gynecology Hepatology Immunohistochemistry Inflammation - metabolism Inflammation - pathology Interstitial Cells of Cajal - metabolism Intervention Intestinal Mucosa - pathology Medicine Medicine & Public Health Mortality Motility Pregnancy Proctology Sheep Smooth muscle Surgery |
| title | Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis |
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