Effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells
The effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells was studied by cytochemical methods. A 6-hr exposure to cadmium (10 μm) in a buffered salt solution resulted in the breakdown of actin filaments, particularly those associated with both the stress fibers and the lat...
Gespeichert in:
| Veröffentlicht in: | Toxicology and applied pharmacology 1989-11, Vol.101 (2), p.245-254 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 254 |
|---|---|
| container_issue | 2 |
| container_start_page | 245 |
| container_title | Toxicology and applied pharmacology |
| container_volume | 101 |
| creator | Mills, John W. Ferm, Vergil H. |
| description | The effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells was studied by cytochemical methods. A 6-hr exposure to cadmium (10 μm) in a buffered salt solution resulted in the breakdown of actin filaments, particularly those associated with both the stress fibers and the lateral membranes in areas of intercellular contact. Microtubules were not dramatically altered during this exposure period and cell viability, determined by trypan blue exclusion, was similar to controls. The effect of cadmium on actin was reversible if the cells were returned to culture medium. The results indicate that one possible mechanism of cadmium toxicity is via an effect on the organization of actin filaments. |
| doi_str_mv | 10.1016/0041-008X(89)90273-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15417618</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0041008X89902731</els_id><sourcerecordid>15417618</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-e7f5adb69fa52d81d1be50987b7aa2b59afdd48732de3637c3522ead907f7d843</originalsourceid><addsrcrecordid>eNp9kEtv1DAURi0EaqeFf0AlLxBqFwE7TvzYIKFpC4giWIDEzrqxryVXidPaCdL8e5LOaJZd3cV37usQ8pazD5xx-ZGxhleM6b-X2lwZVitR8Rdkw5mRFRNCvCSbI3JKzkq5Z4yZpuEn5KSWWtRcbsivmxDQTXQM1IEf4jzQMdHbCtwUE4Xk6RBdHqe5m3ssK_YDfEzVNeRuR7eQYkL6PfqEO-qw78tr8ipAX_DNoZ6TP7c3v7dfq7ufX75tP99VruFqqlCFFnwnTYC29pp73mHLjFadAqi71kDwvtFK1B6FFMqJtq4RvGEqKK8bcU7e7-c-5PFxxjLZIZb1Akg4zsXydtkjuV7AZg8ub5SSMdiHHAfIO8uZXUXa1ZJdLVlt7JNIy5e2i8P8uRvQH5sO5pb83SGH4qAPGZKL5YhJ2WgmV-zTHsPFxb-I2RYXMTn0MS_erR_j83f8Bz0Ojk4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15417618</pqid></control><display><type>article</type><title>Effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Mills, John W. ; Ferm, Vergil H.</creator><creatorcontrib>Mills, John W. ; Ferm, Vergil H.</creatorcontrib><description>The effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells was studied by cytochemical methods. A 6-hr exposure to cadmium (10 μm) in a buffered salt solution resulted in the breakdown of actin filaments, particularly those associated with both the stress fibers and the lateral membranes in areas of intercellular contact. Microtubules were not dramatically altered during this exposure period and cell viability, determined by trypan blue exclusion, was similar to controls. The effect of cadmium on actin was reversible if the cells were returned to culture medium. The results indicate that one possible mechanism of cadmium toxicity is via an effect on the organization of actin filaments.</description><identifier>ISSN: 0041-008X</identifier><identifier>EISSN: 1096-0333</identifier><identifier>DOI: 10.1016/0041-008X(89)90273-1</identifier><identifier>PMID: 2683216</identifier><identifier>CODEN: TXAPA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Actins - metabolism ; Animals ; Biological and medical sciences ; Cadmium - toxicity ; Cells, Cultured ; Chemical and industrial products toxicology. Toxic occupational diseases ; Cytochalasin B - pharmacology ; Dogs ; Histocytochemistry ; Kidney - drug effects ; Kidney - metabolism ; Kidney - ultrastructure ; Medical sciences ; Metals and various inorganic compounds ; Microscopy, Phase-Contrast ; Microtubules - drug effects ; Microtubules - metabolism ; Microtubules - ultrastructure ; Toxicology</subject><ispartof>Toxicology and applied pharmacology, 1989-11, Vol.101 (2), p.245-254</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e7f5adb69fa52d81d1be50987b7aa2b59afdd48732de3637c3522ead907f7d843</citedby><cites>FETCH-LOGICAL-c417t-e7f5adb69fa52d81d1be50987b7aa2b59afdd48732de3637c3522ead907f7d843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0041-008X(89)90273-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6648066$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2683216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mills, John W.</creatorcontrib><creatorcontrib>Ferm, Vergil H.</creatorcontrib><title>Effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells</title><title>Toxicology and applied pharmacology</title><addtitle>Toxicol Appl Pharmacol</addtitle><description>The effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells was studied by cytochemical methods. A 6-hr exposure to cadmium (10 μm) in a buffered salt solution resulted in the breakdown of actin filaments, particularly those associated with both the stress fibers and the lateral membranes in areas of intercellular contact. Microtubules were not dramatically altered during this exposure period and cell viability, determined by trypan blue exclusion, was similar to controls. The effect of cadmium on actin was reversible if the cells were returned to culture medium. The results indicate that one possible mechanism of cadmium toxicity is via an effect on the organization of actin filaments.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cadmium - toxicity</subject><subject>Cells, Cultured</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Cytochalasin B - pharmacology</subject><subject>Dogs</subject><subject>Histocytochemistry</subject><subject>Kidney - drug effects</subject><subject>Kidney - metabolism</subject><subject>Kidney - ultrastructure</subject><subject>Medical sciences</subject><subject>Metals and various inorganic compounds</subject><subject>Microscopy, Phase-Contrast</subject><subject>Microtubules - drug effects</subject><subject>Microtubules - metabolism</subject><subject>Microtubules - ultrastructure</subject><subject>Toxicology</subject><issn>0041-008X</issn><issn>1096-0333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1DAURi0EaqeFf0AlLxBqFwE7TvzYIKFpC4giWIDEzrqxryVXidPaCdL8e5LOaJZd3cV37usQ8pazD5xx-ZGxhleM6b-X2lwZVitR8Rdkw5mRFRNCvCSbI3JKzkq5Z4yZpuEn5KSWWtRcbsivmxDQTXQM1IEf4jzQMdHbCtwUE4Xk6RBdHqe5m3ssK_YDfEzVNeRuR7eQYkL6PfqEO-qw78tr8ipAX_DNoZ6TP7c3v7dfq7ufX75tP99VruFqqlCFFnwnTYC29pp73mHLjFadAqi71kDwvtFK1B6FFMqJtq4RvGEqKK8bcU7e7-c-5PFxxjLZIZb1Akg4zsXydtkjuV7AZg8ub5SSMdiHHAfIO8uZXUXa1ZJdLVlt7JNIy5e2i8P8uRvQH5sO5pb83SGH4qAPGZKL5YhJ2WgmV-zTHsPFxb-I2RYXMTn0MS_erR_j83f8Bz0Ojk4</recordid><startdate>19891101</startdate><enddate>19891101</enddate><creator>Mills, John W.</creator><creator>Ferm, Vergil H.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19891101</creationdate><title>Effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells</title><author>Mills, John W. ; Ferm, Vergil H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e7f5adb69fa52d81d1be50987b7aa2b59afdd48732de3637c3522ead907f7d843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cadmium - toxicity</topic><topic>Cells, Cultured</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Cytochalasin B - pharmacology</topic><topic>Dogs</topic><topic>Histocytochemistry</topic><topic>Kidney - drug effects</topic><topic>Kidney - metabolism</topic><topic>Kidney - ultrastructure</topic><topic>Medical sciences</topic><topic>Metals and various inorganic compounds</topic><topic>Microscopy, Phase-Contrast</topic><topic>Microtubules - drug effects</topic><topic>Microtubules - metabolism</topic><topic>Microtubules - ultrastructure</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mills, John W.</creatorcontrib><creatorcontrib>Ferm, Vergil H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology and applied pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mills, John W.</au><au>Ferm, Vergil H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells</atitle><jtitle>Toxicology and applied pharmacology</jtitle><addtitle>Toxicol Appl Pharmacol</addtitle><date>1989-11-01</date><risdate>1989</risdate><volume>101</volume><issue>2</issue><spage>245</spage><epage>254</epage><pages>245-254</pages><issn>0041-008X</issn><eissn>1096-0333</eissn><coden>TXAPA9</coden><abstract>The effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells was studied by cytochemical methods. A 6-hr exposure to cadmium (10 μm) in a buffered salt solution resulted in the breakdown of actin filaments, particularly those associated with both the stress fibers and the lateral membranes in areas of intercellular contact. Microtubules were not dramatically altered during this exposure period and cell viability, determined by trypan blue exclusion, was similar to controls. The effect of cadmium on actin was reversible if the cells were returned to culture medium. The results indicate that one possible mechanism of cadmium toxicity is via an effect on the organization of actin filaments.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2683216</pmid><doi>10.1016/0041-008X(89)90273-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-008X |
| ispartof | Toxicology and applied pharmacology, 1989-11, Vol.101 (2), p.245-254 |
| issn | 0041-008X 1096-0333 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15417618 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Actins - metabolism Animals Biological and medical sciences Cadmium - toxicity Cells, Cultured Chemical and industrial products toxicology. Toxic occupational diseases Cytochalasin B - pharmacology Dogs Histocytochemistry Kidney - drug effects Kidney - metabolism Kidney - ultrastructure Medical sciences Metals and various inorganic compounds Microscopy, Phase-Contrast Microtubules - drug effects Microtubules - metabolism Microtubules - ultrastructure Toxicology |
| title | Effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T01%3A53%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20cadmium%20on%20F-actin%20and%20microtubules%20of%20Madin-Darby%20Canine%20Kidney%20cells&rft.jtitle=Toxicology%20and%20applied%20pharmacology&rft.au=Mills,%20John%20W.&rft.date=1989-11-01&rft.volume=101&rft.issue=2&rft.spage=245&rft.epage=254&rft.pages=245-254&rft.issn=0041-008X&rft.eissn=1096-0333&rft.coden=TXAPA9&rft_id=info:doi/10.1016/0041-008X(89)90273-1&rft_dat=%3Cproquest_cross%3E15417618%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15417618&rft_id=info:pmid/2683216&rft_els_id=0041008X89902731&rfr_iscdi=true |