SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide-antibody assays
Ag nanoparticles synthesized on porous silicon samples were studied and applied as substrates for surface‐enhanced Raman scattering (SERS). The metallic nanostructures prepared by immersion plating were characterized by UV–Vis reflectance spectroscopy and scanning electron microscopy. SERS activity...
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| Veröffentlicht in: | Journal of Raman spectroscopy 2012-06, Vol.43 (6), p.730-736 |
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| container_title | Journal of Raman spectroscopy |
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| creator | Virga, Alessandro Rivolo, Paola Descrovi, Emiliano Chiolerio, Alessandro Digregorio, Gabriella Frascella, Francesca Soster, Marco Bussolino, Federico Marchiò, Serena Geobaldo, Francesco Giorgis, Fabrizio |
| description | Ag nanoparticles synthesized on porous silicon samples were studied and applied as substrates for surface‐enhanced Raman scattering (SERS). The metallic nanostructures prepared by immersion plating were characterized by UV–Vis reflectance spectroscopy and scanning electron microscopy. SERS activity of the substrates was tested using Cyanine dye 1,3,3,1′,3′,3′‐esamethyl‐5,5′‐dimethoxyindodicarbocyanine iodide (Cy5‐OCH3) as a probe molecule. The Raman spectra obtained for different excitation wavelengths indicate amplifications ascribed to plasmonic resonances with an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays. Copyright © 2011 John Wiley & Sons, Ltd.
Ag nanoparticles synthesized on porous silicon samples were studied and applied as surface‐enhanced Raman scattering (SERS) substrates. SERS activity was tested using Cy5 dye verifying an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays. |
| doi_str_mv | 10.1002/jrs.3086 |
| format | Article |
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Ag nanoparticles synthesized on porous silicon samples were studied and applied as surface‐enhanced Raman scattering (SERS) substrates. SERS activity was tested using Cy5 dye verifying an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays.</description><identifier>ISSN: 0377-0486</identifier><identifier>EISSN: 1097-4555</identifier><identifier>DOI: 10.1002/jrs.3086</identifier><identifier>CODEN: JRSPAF</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Assaying ; cyanine dye ; Excitation ; Nanoparticles ; peptide-antibody assays ; Peptides ; Plasmonics ; Porous silicon ; Raman scattering ; SERS ; Silver</subject><ispartof>Journal of Raman spectroscopy, 2012-06, Vol.43 (6), p.730-736</ispartof><rights>Copyright © 2011 John Wiley & Sons, Ltd.</rights><rights>Copyright © 2012 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4306-1965d43633b4c6d2ec5ebd249c805bfc493ff17c37fda557913210d4b0e07c3d3</citedby><cites>FETCH-LOGICAL-c4306-1965d43633b4c6d2ec5ebd249c805bfc493ff17c37fda557913210d4b0e07c3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjrs.3086$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjrs.3086$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Virga, Alessandro</creatorcontrib><creatorcontrib>Rivolo, Paola</creatorcontrib><creatorcontrib>Descrovi, Emiliano</creatorcontrib><creatorcontrib>Chiolerio, Alessandro</creatorcontrib><creatorcontrib>Digregorio, Gabriella</creatorcontrib><creatorcontrib>Frascella, Francesca</creatorcontrib><creatorcontrib>Soster, Marco</creatorcontrib><creatorcontrib>Bussolino, Federico</creatorcontrib><creatorcontrib>Marchiò, Serena</creatorcontrib><creatorcontrib>Geobaldo, Francesco</creatorcontrib><creatorcontrib>Giorgis, Fabrizio</creatorcontrib><title>SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide-antibody assays</title><title>Journal of Raman spectroscopy</title><addtitle>J. Raman Spectrosc</addtitle><description>Ag nanoparticles synthesized on porous silicon samples were studied and applied as substrates for surface‐enhanced Raman scattering (SERS). The metallic nanostructures prepared by immersion plating were characterized by UV–Vis reflectance spectroscopy and scanning electron microscopy. SERS activity of the substrates was tested using Cyanine dye 1,3,3,1′,3′,3′‐esamethyl‐5,5′‐dimethoxyindodicarbocyanine iodide (Cy5‐OCH3) as a probe molecule. The Raman spectra obtained for different excitation wavelengths indicate amplifications ascribed to plasmonic resonances with an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays. Copyright © 2011 John Wiley & Sons, Ltd.
Ag nanoparticles synthesized on porous silicon samples were studied and applied as surface‐enhanced Raman scattering (SERS) substrates. SERS activity was tested using Cy5 dye verifying an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays.</description><subject>Assaying</subject><subject>cyanine dye</subject><subject>Excitation</subject><subject>Nanoparticles</subject><subject>peptide-antibody assays</subject><subject>Peptides</subject><subject>Plasmonics</subject><subject>Porous silicon</subject><subject>Raman scattering</subject><subject>SERS</subject><subject>Silver</subject><issn>0377-0486</issn><issn>1097-4555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kE1rGzEQhkVJoY5T6E8Q9JLLpiNLWnlzM_lwWvIBcUqOQivNGrlraSOt2_rfd41LQgI5DQzP-zLzEPKFwQkDmHxbpXzCYVp-ICMGlSqElPKAjIArVYCYlp_IYc4rAKiqko1IWFzcL6ixvf-NdLakwYTYmdR722KmPtA15tjFFDeZZt96G8MpddjjkIiBxobGtDTBW7qOLdrNLmWCox12vXdYmND7OrotNTmbbT4iHxvTZvz8f47Jz8uLh7Or4vpu_v1sdl1YwaEsWFVKJ3jJeS1s6SZoJdZuIio7BVk3VlS8aZiyXDXOSKkqxicMnKgBYdg6PibH-94uxacN5l6vfbbYtibg8IpmUrCdGSUG9OsbdBU3KQzXDRRTAjhX_KXQpphzwkZ3ya9N2moGeideD-L1TvyAFnv0j29x-y6nf9wvXvM-9_j3mTfply4VV1I_3s71-fzx9ko-3GjJ_wF5S5QN</recordid><startdate>201206</startdate><enddate>201206</enddate><creator>Virga, Alessandro</creator><creator>Rivolo, Paola</creator><creator>Descrovi, Emiliano</creator><creator>Chiolerio, Alessandro</creator><creator>Digregorio, Gabriella</creator><creator>Frascella, Francesca</creator><creator>Soster, Marco</creator><creator>Bussolino, Federico</creator><creator>Marchiò, Serena</creator><creator>Geobaldo, Francesco</creator><creator>Giorgis, Fabrizio</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201206</creationdate><title>SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide-antibody assays</title><author>Virga, Alessandro ; Rivolo, Paola ; Descrovi, Emiliano ; Chiolerio, Alessandro ; Digregorio, Gabriella ; Frascella, Francesca ; Soster, Marco ; Bussolino, Federico ; Marchiò, Serena ; Geobaldo, Francesco ; Giorgis, Fabrizio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4306-1965d43633b4c6d2ec5ebd249c805bfc493ff17c37fda557913210d4b0e07c3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Assaying</topic><topic>cyanine dye</topic><topic>Excitation</topic><topic>Nanoparticles</topic><topic>peptide-antibody assays</topic><topic>Peptides</topic><topic>Plasmonics</topic><topic>Porous silicon</topic><topic>Raman scattering</topic><topic>SERS</topic><topic>Silver</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Virga, Alessandro</creatorcontrib><creatorcontrib>Rivolo, Paola</creatorcontrib><creatorcontrib>Descrovi, Emiliano</creatorcontrib><creatorcontrib>Chiolerio, Alessandro</creatorcontrib><creatorcontrib>Digregorio, Gabriella</creatorcontrib><creatorcontrib>Frascella, Francesca</creatorcontrib><creatorcontrib>Soster, Marco</creatorcontrib><creatorcontrib>Bussolino, Federico</creatorcontrib><creatorcontrib>Marchiò, Serena</creatorcontrib><creatorcontrib>Geobaldo, Francesco</creatorcontrib><creatorcontrib>Giorgis, Fabrizio</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of Raman spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Virga, Alessandro</au><au>Rivolo, Paola</au><au>Descrovi, Emiliano</au><au>Chiolerio, Alessandro</au><au>Digregorio, Gabriella</au><au>Frascella, Francesca</au><au>Soster, Marco</au><au>Bussolino, Federico</au><au>Marchiò, Serena</au><au>Geobaldo, Francesco</au><au>Giorgis, Fabrizio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide-antibody assays</atitle><jtitle>Journal of Raman spectroscopy</jtitle><addtitle>J. Raman Spectrosc</addtitle><date>2012-06</date><risdate>2012</risdate><volume>43</volume><issue>6</issue><spage>730</spage><epage>736</epage><pages>730-736</pages><issn>0377-0486</issn><eissn>1097-4555</eissn><coden>JRSPAF</coden><abstract>Ag nanoparticles synthesized on porous silicon samples were studied and applied as substrates for surface‐enhanced Raman scattering (SERS). The metallic nanostructures prepared by immersion plating were characterized by UV–Vis reflectance spectroscopy and scanning electron microscopy. SERS activity of the substrates was tested using Cyanine dye 1,3,3,1′,3′,3′‐esamethyl‐5,5′‐dimethoxyindodicarbocyanine iodide (Cy5‐OCH3) as a probe molecule. The Raman spectra obtained for different excitation wavelengths indicate amplifications ascribed to plasmonic resonances with an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays. Copyright © 2011 John Wiley & Sons, Ltd.
Ag nanoparticles synthesized on porous silicon samples were studied and applied as surface‐enhanced Raman scattering (SERS) substrates. SERS activity was tested using Cy5 dye verifying an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jrs.3086</doi><tpages>7</tpages></addata></record> |
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| subjects | Assaying cyanine dye Excitation Nanoparticles peptide-antibody assays Peptides Plasmonics Porous silicon Raman scattering SERS Silver |
| title | SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide-antibody assays |
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