Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. T...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2014-05, Vol.50 (40), p.5313-5315
Hauptverfasser:	He, Hong-Zhang, Leung, Ka-Ho, Wang, Wei, Chan, Daniel Shiu-Hin, Leung, Chung-Hang, Ma, Dik-Lung
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Biological Assay Biosensing Techniques Coordination Complexes - chemistry DNA Probes - chemistry DNA, Single-Stranded - chemistry G-Quadruplexes Humans Inhibitors Iridium - chemistry Kinases Luminescence Luminescent Measurements Phosphorylation Polynucleotide 5'-Hydroxyl-Kinase - metabolism Polynucleotides Screening
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Zusammenfassung:	A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c3cc47444e