Characterization of Hg-phytochelatins complexes in vines (Vitis vinifera cv Malbec) as defense mechanism against metal stress
An approach to understand vines ( Vitis vinifera ) defense mechanism against heavy metal stress by isolation and determination of Hg-phytochelatins (PCs) complexes was performed. PCs are important molecules involved in the control of metal concentration in plants. PCs complex toxic metals through −S...
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| Veröffentlicht in: | Biometals 2014-06, Vol.27 (3), p.591-599 |
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| creator | Spisso, Adrian A. Cerutti, Soledad Silva, Fernanda Pacheco, Pablo H. Martinez, Luis D. |
| description | An approach to understand vines (
Vitis vinifera
) defense mechanism against heavy metal stress by isolation and determination of Hg-phytochelatins (PCs) complexes was performed. PCs are important molecules involved in the control of metal concentration in plants. PCs complex toxic metals through −SH groups and stores them inside cells vacuole avoiding any toxic effect of free metals in the cytosol. The Hg-PCs identification was achieved by determination of Hg and S as hetero-tagged atoms. A method involving two-dimensional chromatographic analysis coupled to atomic spectrometry and confirmation by tandem mass spectrometry is proposed. An approach involving size exclusion chromatography coupled to inductively coupled plasma mass spectrometry on roots, stems, and leaves extracts describing Hg distribution according to molecular weight and sulfur associations is proposed for the first time. Medium–low molecular weight Hg–S associations of 29–100 kDa were found, suggesting PCs presence. A second approach employing reversed-phase chromatography coupled to atomic fluorescence spectrometry analysis allowed the determination of Hg-PCs complexes within the mentioned fractions. Chromatograms showed Hg-PC
2
, Hg-PC
3
and Hg-PC
4
presence only in roots. Hg-PCs presence in roots was confirmed by ESI–MS/MS analysis. |
| doi_str_mv | 10.1007/s10534-014-9732-9 |
| format | Article |
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Vitis vinifera
) defense mechanism against heavy metal stress by isolation and determination of Hg-phytochelatins (PCs) complexes was performed. PCs are important molecules involved in the control of metal concentration in plants. PCs complex toxic metals through −SH groups and stores them inside cells vacuole avoiding any toxic effect of free metals in the cytosol. The Hg-PCs identification was achieved by determination of Hg and S as hetero-tagged atoms. A method involving two-dimensional chromatographic analysis coupled to atomic spectrometry and confirmation by tandem mass spectrometry is proposed. An approach involving size exclusion chromatography coupled to inductively coupled plasma mass spectrometry on roots, stems, and leaves extracts describing Hg distribution according to molecular weight and sulfur associations is proposed for the first time. Medium–low molecular weight Hg–S associations of 29–100 kDa were found, suggesting PCs presence. A second approach employing reversed-phase chromatography coupled to atomic fluorescence spectrometry analysis allowed the determination of Hg-PCs complexes within the mentioned fractions. Chromatograms showed Hg-PC
2
, Hg-PC
3
and Hg-PC
4
presence only in roots. Hg-PCs presence in roots was confirmed by ESI–MS/MS analysis.</description><identifier>ISSN: 0966-0844</identifier><identifier>EISSN: 1572-8773</identifier><identifier>DOI: 10.1007/s10534-014-9732-9</identifier><identifier>PMID: 24715273</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adaptation, Physiological ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Chromatography ; Chromatography, High Pressure Liquid ; Chromatography, Reverse-Phase ; Coordination Complexes - isolation & purification ; Coordination Complexes - metabolism ; Heavy metals ; Life Sciences ; Mass spectrometry ; Medicine/Public Health ; Mercury ; Mercury (metal) ; Mercury - isolation & purification ; Mercury - metabolism ; Metal concentrations ; Metals ; Microbiology ; Molecular weight ; Pharmacology/Toxicology ; Phytochelatins - isolation & purification ; Phytochelatins - metabolism ; Plant Leaves - metabolism ; Plant Physiology ; Plant resistance ; Plant Roots - metabolism ; Plant Stems - metabolism ; Psychological effects ; Roots ; Spectroscopy ; Stress, Physiological ; Sulfur ; Toxic ; Vitis - metabolism</subject><ispartof>Biometals, 2014-06, Vol.27 (3), p.591-599</ispartof><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-178ff1fac2cacb1eae07c5168c2009a2eba3f9bd8fbd193698b0c282883b40243</citedby><cites>FETCH-LOGICAL-c448t-178ff1fac2cacb1eae07c5168c2009a2eba3f9bd8fbd193698b0c282883b40243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10534-014-9732-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10534-014-9732-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24715273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spisso, Adrian A.</creatorcontrib><creatorcontrib>Cerutti, Soledad</creatorcontrib><creatorcontrib>Silva, Fernanda</creatorcontrib><creatorcontrib>Pacheco, Pablo H.</creatorcontrib><creatorcontrib>Martinez, Luis D.</creatorcontrib><title>Characterization of Hg-phytochelatins complexes in vines (Vitis vinifera cv Malbec) as defense mechanism against metal stress</title><title>Biometals</title><addtitle>Biometals</addtitle><addtitle>Biometals</addtitle><description>An approach to understand vines (
Vitis vinifera
) defense mechanism against heavy metal stress by isolation and determination of Hg-phytochelatins (PCs) complexes was performed. PCs are important molecules involved in the control of metal concentration in plants. PCs complex toxic metals through −SH groups and stores them inside cells vacuole avoiding any toxic effect of free metals in the cytosol. The Hg-PCs identification was achieved by determination of Hg and S as hetero-tagged atoms. A method involving two-dimensional chromatographic analysis coupled to atomic spectrometry and confirmation by tandem mass spectrometry is proposed. An approach involving size exclusion chromatography coupled to inductively coupled plasma mass spectrometry on roots, stems, and leaves extracts describing Hg distribution according to molecular weight and sulfur associations is proposed for the first time. Medium–low molecular weight Hg–S associations of 29–100 kDa were found, suggesting PCs presence. A second approach employing reversed-phase chromatography coupled to atomic fluorescence spectrometry analysis allowed the determination of Hg-PCs complexes within the mentioned fractions. Chromatograms showed Hg-PC
2
, Hg-PC
3
and Hg-PC
4
presence only in roots. Hg-PCs presence in roots was confirmed by ESI–MS/MS analysis.</description><subject>Adaptation, Physiological</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Reverse-Phase</subject><subject>Coordination Complexes - isolation & purification</subject><subject>Coordination Complexes - metabolism</subject><subject>Heavy metals</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Medicine/Public Health</subject><subject>Mercury</subject><subject>Mercury (metal)</subject><subject>Mercury - isolation & purification</subject><subject>Mercury - metabolism</subject><subject>Metal concentrations</subject><subject>Metals</subject><subject>Microbiology</subject><subject>Molecular weight</subject><subject>Pharmacology/Toxicology</subject><subject>Phytochelatins - isolation & purification</subject><subject>Phytochelatins - metabolism</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Physiology</subject><subject>Plant resistance</subject><subject>Plant Roots - metabolism</subject><subject>Plant Stems - metabolism</subject><subject>Psychological effects</subject><subject>Roots</subject><subject>Spectroscopy</subject><subject>Stress, Physiological</subject><subject>Sulfur</subject><subject>Toxic</subject><subject>Vitis - 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Pablo H.</au><au>Martinez, Luis D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Hg-phytochelatins complexes in vines (Vitis vinifera cv Malbec) as defense mechanism against metal stress</atitle><jtitle>Biometals</jtitle><stitle>Biometals</stitle><addtitle>Biometals</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>27</volume><issue>3</issue><spage>591</spage><epage>599</epage><pages>591-599</pages><issn>0966-0844</issn><eissn>1572-8773</eissn><abstract>An approach to understand vines (
Vitis vinifera
) defense mechanism against heavy metal stress by isolation and determination of Hg-phytochelatins (PCs) complexes was performed. PCs are important molecules involved in the control of metal concentration in plants. PCs complex toxic metals through −SH groups and stores them inside cells vacuole avoiding any toxic effect of free metals in the cytosol. The Hg-PCs identification was achieved by determination of Hg and S as hetero-tagged atoms. A method involving two-dimensional chromatographic analysis coupled to atomic spectrometry and confirmation by tandem mass spectrometry is proposed. An approach involving size exclusion chromatography coupled to inductively coupled plasma mass spectrometry on roots, stems, and leaves extracts describing Hg distribution according to molecular weight and sulfur associations is proposed for the first time. Medium–low molecular weight Hg–S associations of 29–100 kDa were found, suggesting PCs presence. A second approach employing reversed-phase chromatography coupled to atomic fluorescence spectrometry analysis allowed the determination of Hg-PCs complexes within the mentioned fractions. Chromatograms showed Hg-PC
2
, Hg-PC
3
and Hg-PC
4
presence only in roots. Hg-PCs presence in roots was confirmed by ESI–MS/MS analysis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24715273</pmid><doi>10.1007/s10534-014-9732-9</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biometals, 2014-06, Vol.27 (3), p.591-599 |
| issn | 0966-0844 1572-8773 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Adaptation, Physiological Biochemistry Biomedical and Life Sciences Cell Biology Chromatography Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Coordination Complexes - isolation & purification Coordination Complexes - metabolism Heavy metals Life Sciences Mass spectrometry Medicine/Public Health Mercury Mercury (metal) Mercury - isolation & purification Mercury - metabolism Metal concentrations Metals Microbiology Molecular weight Pharmacology/Toxicology Phytochelatins - isolation & purification Phytochelatins - metabolism Plant Leaves - metabolism Plant Physiology Plant resistance Plant Roots - metabolism Plant Stems - metabolism Psychological effects Roots Spectroscopy Stress, Physiological Sulfur Toxic Vitis - metabolism |
| title | Characterization of Hg-phytochelatins complexes in vines (Vitis vinifera cv Malbec) as defense mechanism against metal stress |
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