Determination of polyamines by precolumn derivatization with 9-fluorenylmethyl chloroformate and reverse-phase high-performance liquid chromatography

A high-performance liquid chromatography (HPLC) method for the determination of picomole levels of polyamines (putrescine, spermidine, and spermine) is described. Amino groups in polyamines react with 9-fluorenylmethyl chloroformate (FMOC) to form stable and highly fluorescent derivatives which can...

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Veröffentlicht in:	Journal of neuroscience methods 1989-07, Vol.29 (1), p.27-31
Hauptverfasser:	Sabri, Mohammad I., Soiefer, Andrew I., Kisby, Glen E., Spencer, Peter S.
Format:	Artikel
Sprache:	eng
Schlagworte:	9-Fluorenylmethyl chloroformate Animals Biochemistry and metabolism Biogenic Polyamines - analysis Biological and medical sciences Central nervous system Chromatography, High Pressure Liquid - methods Dorsal root ganglion Fluorenes Fundamental and applied biological sciences. Psychology Ganglia, Spinal - analysis High-performance liquid chromatography Polyamine Putrescine - analysis Rats Rats, Inbred Strains Spermidine - analysis Spermine - analysis Vertebrates: nervous system and sense organs
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