Determination of polyamines by precolumn derivatization with 9-fluorenylmethyl chloroformate and reverse-phase high-performance liquid chromatography
A high-performance liquid chromatography (HPLC) method for the determination of picomole levels of polyamines (putrescine, spermidine, and spermine) is described. Amino groups in polyamines react with 9-fluorenylmethyl chloroformate (FMOC) to form stable and highly fluorescent derivatives which can...
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| Veröffentlicht in: | Journal of neuroscience methods 1989-07, Vol.29 (1), p.27-31 |
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| creator | Sabri, Mohammad I. Soiefer, Andrew I. Kisby, Glen E. Spencer, Peter S. |
| description | A high-performance liquid chromatography (HPLC) method for the determination of picomole levels of polyamines (putrescine, spermidine, and spermine) is described. Amino groups in polyamines react with 9-fluorenylmethyl chloroformate (FMOC) to form stable and highly fluorescent derivatives which can be separated and quantitatively estimated by HPLC in about 12 min. The mean relative elution times (
n = 14) for putrescine, spermidine and spermine are 4.21 ± 0.02, 10.09 ± 0.02 and 11.19 ± 0.04 min. respectively. The method has been applied to determine polyamine concentration in rat dorsal root ganglia (DRG) without interference with endogenous amino acids. Polyamine content of individual rat DRG has been calculated and the values are as follows: putrescine, 36.8 ± 2.01, spermidine, 1652 ± 131.0 and spermine 388.5 ± 38.4 pmol/DRG. Information on polyamine concentrations in DRG may be useful in understanding the mechanism of action of toxic chemicals on nervous system. |
| doi_str_mv | 10.1016/0165-0270(89)90105-2 |
| format | Article |
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n = 14) for putrescine, spermidine and spermine are 4.21 ± 0.02, 10.09 ± 0.02 and 11.19 ± 0.04 min. respectively. The method has been applied to determine polyamine concentration in rat dorsal root ganglia (DRG) without interference with endogenous amino acids. Polyamine content of individual rat DRG has been calculated and the values are as follows: putrescine, 36.8 ± 2.01, spermidine, 1652 ± 131.0 and spermine 388.5 ± 38.4 pmol/DRG. Information on polyamine concentrations in DRG may be useful in understanding the mechanism of action of toxic chemicals on nervous system.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/0165-0270(89)90105-2</identifier><identifier>PMID: 2761296</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>9-Fluorenylmethyl chloroformate ; Animals ; Biochemistry and metabolism ; Biogenic Polyamines - analysis ; Biological and medical sciences ; Central nervous system ; Chromatography, High Pressure Liquid - methods ; Dorsal root ganglion ; Fluorenes ; Fundamental and applied biological sciences. Psychology ; Ganglia, Spinal - analysis ; High-performance liquid chromatography ; Polyamine ; Putrescine - analysis ; Rats ; Rats, Inbred Strains ; Spermidine - analysis ; Spermine - analysis ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 1989-07, Vol.29 (1), p.27-31</ispartof><rights>1989</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-bb07fd050db6e4b41fd47fae216a581ba5f5b830a07384b2e5ac8a332941b3153</citedby><cites>FETCH-LOGICAL-c464t-bb07fd050db6e4b41fd47fae216a581ba5f5b830a07384b2e5ac8a332941b3153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-0270(89)90105-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19610486$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2761296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabri, Mohammad I.</creatorcontrib><creatorcontrib>Soiefer, Andrew I.</creatorcontrib><creatorcontrib>Kisby, Glen E.</creatorcontrib><creatorcontrib>Spencer, Peter S.</creatorcontrib><title>Determination of polyamines by precolumn derivatization with 9-fluorenylmethyl chloroformate and reverse-phase high-performance liquid chromatography</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>A high-performance liquid chromatography (HPLC) method for the determination of picomole levels of polyamines (putrescine, spermidine, and spermine) is described. Amino groups in polyamines react with 9-fluorenylmethyl chloroformate (FMOC) to form stable and highly fluorescent derivatives which can be separated and quantitatively estimated by HPLC in about 12 min. The mean relative elution times (
n = 14) for putrescine, spermidine and spermine are 4.21 ± 0.02, 10.09 ± 0.02 and 11.19 ± 0.04 min. respectively. The method has been applied to determine polyamine concentration in rat dorsal root ganglia (DRG) without interference with endogenous amino acids. Polyamine content of individual rat DRG has been calculated and the values are as follows: putrescine, 36.8 ± 2.01, spermidine, 1652 ± 131.0 and spermine 388.5 ± 38.4 pmol/DRG. Information on polyamine concentrations in DRG may be useful in understanding the mechanism of action of toxic chemicals on nervous system.</description><subject>9-Fluorenylmethyl chloroformate</subject><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biogenic Polyamines - analysis</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dorsal root ganglion</subject><subject>Fluorenes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglia, Spinal - analysis</subject><subject>High-performance liquid chromatography</subject><subject>Polyamine</subject><subject>Putrescine - analysis</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Spermidine - analysis</subject><subject>Spermine - analysis</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2L1TAUhoMo453Rf6CQjaKL6kmbpulGkHH8gAE3Cu5C0p5MI2nTSdor9X_4f82dexl3LkIg53kP4XkJecbgDQMm3uZTF1A28Eq2r1tgUBflA7JjsikL0cgfD8nuHnlMzlP6CQC8BXFGzspGsLIVO_LnAy4YRzfpxYWJBkvn4DedHzBRs9E5Yhf8Ok60x-j2mfp9JH-5ZaBtYf0aIk6bH3EZNk-7wYcYbIijXpDqqacR9xgTFvOgE9LB3QzFjPGOmDqk3t2urs-5GHIk3EQ9D9sT8shqn_Dp6b4g3z9efbv8XFx__fTl8v110XHBl8IYaGwPNfRGIDec2Z43VmPJhK4lM7q2tZEVaGgqyU2Jte6krqqy5cxUrK4uyMvj3jmG2xXTokaXOvReTxjWpFjNQQoOGeRHsIshpYhWzdGNOm6KgTq0oQ6q1UG1kq26a0OVOfb8tH81I_b3oZP-PH9xmuvUaW9jVuLSv92tYMDlgXt35DDL2DuMKnUOs77e5X4W1Qf3_4_8BTQTqxU</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Sabri, Mohammad I.</creator><creator>Soiefer, Andrew I.</creator><creator>Kisby, Glen E.</creator><creator>Spencer, Peter S.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19890701</creationdate><title>Determination of polyamines by precolumn derivatization with 9-fluorenylmethyl chloroformate and reverse-phase high-performance liquid chromatography</title><author>Sabri, Mohammad I. ; Soiefer, Andrew I. ; Kisby, Glen E. ; Spencer, Peter S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-bb07fd050db6e4b41fd47fae216a581ba5f5b830a07384b2e5ac8a332941b3153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>9-Fluorenylmethyl chloroformate</topic><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biogenic Polyamines - analysis</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dorsal root ganglion</topic><topic>Fluorenes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglia, Spinal - analysis</topic><topic>High-performance liquid chromatography</topic><topic>Polyamine</topic><topic>Putrescine - analysis</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Spermidine - analysis</topic><topic>Spermine - analysis</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabri, Mohammad I.</creatorcontrib><creatorcontrib>Soiefer, Andrew I.</creatorcontrib><creatorcontrib>Kisby, Glen E.</creatorcontrib><creatorcontrib>Spencer, Peter S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabri, Mohammad I.</au><au>Soiefer, Andrew I.</au><au>Kisby, Glen E.</au><au>Spencer, Peter S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of polyamines by precolumn derivatization with 9-fluorenylmethyl chloroformate and reverse-phase high-performance liquid chromatography</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>29</volume><issue>1</issue><spage>27</spage><epage>31</epage><pages>27-31</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>A high-performance liquid chromatography (HPLC) method for the determination of picomole levels of polyamines (putrescine, spermidine, and spermine) is described. Amino groups in polyamines react with 9-fluorenylmethyl chloroformate (FMOC) to form stable and highly fluorescent derivatives which can be separated and quantitatively estimated by HPLC in about 12 min. The mean relative elution times (
n = 14) for putrescine, spermidine and spermine are 4.21 ± 0.02, 10.09 ± 0.02 and 11.19 ± 0.04 min. respectively. The method has been applied to determine polyamine concentration in rat dorsal root ganglia (DRG) without interference with endogenous amino acids. Polyamine content of individual rat DRG has been calculated and the values are as follows: putrescine, 36.8 ± 2.01, spermidine, 1652 ± 131.0 and spermine 388.5 ± 38.4 pmol/DRG. Information on polyamine concentrations in DRG may be useful in understanding the mechanism of action of toxic chemicals on nervous system.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2761296</pmid><doi>10.1016/0165-0270(89)90105-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 9-Fluorenylmethyl chloroformate Animals Biochemistry and metabolism Biogenic Polyamines - analysis Biological and medical sciences Central nervous system Chromatography, High Pressure Liquid - methods Dorsal root ganglion Fluorenes Fundamental and applied biological sciences. Psychology Ganglia, Spinal - analysis High-performance liquid chromatography Polyamine Putrescine - analysis Rats Rats, Inbred Strains Spermidine - analysis Spermine - analysis Vertebrates: nervous system and sense organs |
| title | Determination of polyamines by precolumn derivatization with 9-fluorenylmethyl chloroformate and reverse-phase high-performance liquid chromatography |
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