Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deam inase. The chromatographic behaviour of both proteins w...
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| Veröffentlicht in: | Zeitschrift für Naturforschung C. A journal of biosciences 1989-08, Vol.44 (7), p.578-580 |
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| container_issue | 7 |
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| container_title | Zeitschrift für Naturforschung C. A journal of biosciences |
| container_volume | 44 |
| creator | Rossetti, M. V. Juknat, A. A. Batlle, A. M. del C. |
| description | A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase.
The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deam inase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deam inase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000. |
| doi_str_mv | 10.1515/znc-1989-7-807 |
| format | Article |
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The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deam inase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deam inase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000.</description><identifier>ISSN: 0939-5075</identifier><identifier>EISSN: 1865-7125</identifier><identifier>DOI: 10.1515/znc-1989-7-807</identifier><language>eng</language><publisher>Verlag der Zeitschrift für Naturforschung</publisher><subject>chromatography ; Euglena gracilis ; membrane proteins ; Particulate ; Porphobilinogen-Deaminase ; Porphyrins ; Soluble</subject><ispartof>Zeitschrift für Naturforschung C. A journal of biosciences, 1989-08, Vol.44 (7), p.578-580</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-f9471d66997577e0d5c7c2cd95e5fb23e58e31356be2896eaaba89cd0210a35b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Rossetti, M. V.</creatorcontrib><creatorcontrib>Juknat, A. A.</creatorcontrib><creatorcontrib>Batlle, A. M. del C.</creatorcontrib><title>Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis</title><title>Zeitschrift für Naturforschung C. A journal of biosciences</title><description>A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase.
The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deam inase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deam inase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000.</description><subject>chromatography</subject><subject>Euglena gracilis</subject><subject>membrane proteins</subject><subject>Particulate</subject><subject>Porphobilinogen-Deaminase</subject><subject>Porphyrins</subject><subject>Soluble</subject><issn>0939-5075</issn><issn>1865-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNp1kDtPwzAUhS0EEqWwMmdic7Hj3DhekFDLS6oE4jFbjnNTAq5d7ESo_HpSlZXp3OF8R7ofIeeczThwuPzxlnJVKSppxeQBmfCqBCp5DodkwpRQFJiEY3KS0gdjogQJE_L8EtxQO8yMb7InE_vODs70mD2FuHkPdec6H1bo6QLNuvMmYdbGsM4WJn7Suxi-fXYzrBx6k62isWM9nZKj1riEZ385JW-3N6_ze7p8vHuYXy-pFaXoaasKyZuyVEqClMgasNLmtlGA0Na5QKhQcAFljXmlSjSmNpWyDcs5MwJqMSUX-91NDF8Dpl6vu2TROeMxDElzKFjBi2IszvZFG0NKEVu9id3axK3mTO_U6VGd3qnTUo_qRuBqD3wb12NscBWH7XjojzBEP_70D1gUY4CsxC86u3eR</recordid><startdate>19890801</startdate><enddate>19890801</enddate><creator>Rossetti, M. V.</creator><creator>Juknat, A. A.</creator><creator>Batlle, A. M. del C.</creator><general>Verlag der Zeitschrift für Naturforschung</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>M7Z</scope><scope>M81</scope><scope>P64</scope></search><sort><creationdate>19890801</creationdate><title>Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis</title><author>Rossetti, M. V. ; Juknat, A. A. ; Batlle, A. M. del C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-f9471d66997577e0d5c7c2cd95e5fb23e58e31356be2896eaaba89cd0210a35b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>chromatography</topic><topic>Euglena gracilis</topic><topic>membrane proteins</topic><topic>Particulate</topic><topic>Porphobilinogen-Deaminase</topic><topic>Porphyrins</topic><topic>Soluble</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rossetti, M. V.</creatorcontrib><creatorcontrib>Juknat, A. A.</creatorcontrib><creatorcontrib>Batlle, A. M. del C.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Zeitschrift für Naturforschung C. A journal of biosciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rossetti, M. V.</au><au>Juknat, A. A.</au><au>Batlle, A. M. del C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis</atitle><jtitle>Zeitschrift für Naturforschung C. A journal of biosciences</jtitle><date>1989-08-01</date><risdate>1989</risdate><volume>44</volume><issue>7</issue><spage>578</spage><epage>580</epage><pages>578-580</pages><issn>0939-5075</issn><eissn>1865-7125</eissn><abstract>A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase.
The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deam inase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deam inase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000.</abstract><pub>Verlag der Zeitschrift für Naturforschung</pub><doi>10.1515/znc-1989-7-807</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0939-5075 1865-7125 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | chromatography Euglena gracilis membrane proteins Particulate Porphobilinogen-Deaminase Porphyrins Soluble |
| title | Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis |
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