Intact human amniotic membrane differentiated towards the chondrogenic lineage
Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously...
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| Veröffentlicht in: | Cell and tissue banking 2014-06, Vol.15 (2), p.213-225 |
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| creator | Lindenmair, Andrea Nürnberger, Sylvia Stadler, Guido Meinl, Alexandra Hackl, Christa Eibl, Johann Gabriel, Christian Hennerbichler, Simone Redl, Heinz Wolbank, Susanne |
| description | Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hAM represents a natural, preformed sheet including highly potent stem cells. In the present approach for cartilage regeneration we have induced chondrogenesis in hAM in vitro. For this, hAM biopsies were cultured for up to 56 days under chondrogenic conditions. The induced hAM was characterized for remaining viability, glycosaminoglycan (GAG) accumulation using histochemical analysis, and a quantitative assay. Collagen I, II and X was immunohistochemically determined and cartilage-specific mRNA expression of (sex determining region Y-) box 9, cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), versican (CSPG2), COL1A1, COL9A2, melanoma inhibitory activity (MIA), and cartilage-linking protein 1 (CRTL1) analyzed by quantitative real-time polymerase chain reaction. Human AM was successfully induced to accumulate GAG, as demonstrated by Alcianblue staining and a significant (
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| doi_str_mv | 10.1007/s10561-014-9454-9 |
| format | Article |
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p
< 0.001) increase of GAG/viability under chondrogenic conditions peaking in a 29.9 ± 0.9-fold induction on day 56. Further, upon chondrogenic induction collagen II positive areas were identified within histological sections and cartilage-specific markers including COMP, AGC1, CSPG2, COL1A1, COL9A2, MIA, and CRTL1 were found upregulated at mRNA level. This is the first study, demonstrating that upon in vitro induction viable human amnion expresses cartilage-specific markers and accumulates GAGs within the biomatrix. This is a promising first step towards a potential use of living hAM for cartilage TE.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-014-9454-9</identifier><identifier>PMID: 24828570</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amnion - cytology ; Biomedical and Life Sciences ; Biomedicine ; Biopsy ; Cartilage - cytology ; Cell Biology ; Cell culture ; Cell Differentiation - physiology ; Cell Lineage - physiology ; Cells, Cultured ; Chondrogenesis - physiology ; Female ; Humans ; Life Sciences ; Membranes ; Original Paper ; Placenta - cytology ; Pregnancy ; Stem Cells - cytology ; Tissues ; Transplant Surgery</subject><ispartof>Cell and tissue banking, 2014-06, Vol.15 (2), p.213-225</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-71dc902d00be4c1215cf8ed13dea6d74a2f0c07dcea17d8c699f879784fe27883</citedby><cites>FETCH-LOGICAL-c405t-71dc902d00be4c1215cf8ed13dea6d74a2f0c07dcea17d8c699f879784fe27883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-014-9454-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-014-9454-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24828570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lindenmair, Andrea</creatorcontrib><creatorcontrib>Nürnberger, Sylvia</creatorcontrib><creatorcontrib>Stadler, Guido</creatorcontrib><creatorcontrib>Meinl, Alexandra</creatorcontrib><creatorcontrib>Hackl, Christa</creatorcontrib><creatorcontrib>Eibl, Johann</creatorcontrib><creatorcontrib>Gabriel, Christian</creatorcontrib><creatorcontrib>Hennerbichler, Simone</creatorcontrib><creatorcontrib>Redl, Heinz</creatorcontrib><creatorcontrib>Wolbank, Susanne</creatorcontrib><title>Intact human amniotic membrane differentiated towards the chondrogenic lineage</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><addtitle>Cell Tissue Bank</addtitle><description>Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hAM represents a natural, preformed sheet including highly potent stem cells. In the present approach for cartilage regeneration we have induced chondrogenesis in hAM in vitro. For this, hAM biopsies were cultured for up to 56 days under chondrogenic conditions. The induced hAM was characterized for remaining viability, glycosaminoglycan (GAG) accumulation using histochemical analysis, and a quantitative assay. Collagen I, II and X was immunohistochemically determined and cartilage-specific mRNA expression of (sex determining region Y-) box 9, cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), versican (CSPG2), COL1A1, COL9A2, melanoma inhibitory activity (MIA), and cartilage-linking protein 1 (CRTL1) analyzed by quantitative real-time polymerase chain reaction. Human AM was successfully induced to accumulate GAG, as demonstrated by Alcianblue staining and a significant (
p
< 0.001) increase of GAG/viability under chondrogenic conditions peaking in a 29.9 ± 0.9-fold induction on day 56. Further, upon chondrogenic induction collagen II positive areas were identified within histological sections and cartilage-specific markers including COMP, AGC1, CSPG2, COL1A1, COL9A2, MIA, and CRTL1 were found upregulated at mRNA level. This is the first study, demonstrating that upon in vitro induction viable human amnion expresses cartilage-specific markers and accumulates GAGs within the biomatrix. This is a promising first step towards a potential use of living hAM for cartilage TE.</description><subject>Amnion - cytology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biopsy</subject><subject>Cartilage - cytology</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage - physiology</subject><subject>Cells, Cultured</subject><subject>Chondrogenesis - physiology</subject><subject>Female</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Membranes</subject><subject>Original Paper</subject><subject>Placenta - cytology</subject><subject>Pregnancy</subject><subject>Stem Cells - cytology</subject><subject>Tissues</subject><subject>Transplant Surgery</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1rHSEUhiU05Kv9AdmUgW66meYcdUZdltA2gdBukrV49cy9E-44qTqU_Pt6uUkogUI2Kvi871Eexs4RviCAusgIXY8toGyN7OpywE6wU6LtNcp39Sy0aY0Q4pid5nwPwEFxccSOudRcdwpO2M_rWJwvzWaZXGzcFMe5jL6ZaFolF6kJ4zBQolhGVyg0Zf7jUshN2VDjN3MMaV5TrIHtGMmt6T07HNw204en_Yzdff92e3nV3vz6cX359ab1ErrSKgzeAA8AK5IeOXZ-0BRQBHJ9UNLxATyo4MmhCtr3xgxaGaXlQFxpLc7Y533vQ5p_L5SLncbsabutb56XbLGTwAUa_hZUSKm47LGin16h9_OSYv3IjhKG94CmUrinfJpzTjTYhzROLj1aBLvzYvdebPVid17sLvPxqXlZTRReEs8iKsD3QK5XcU3pn9H_bf0Ls5yXrQ</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Lindenmair, Andrea</creator><creator>Nürnberger, Sylvia</creator><creator>Stadler, Guido</creator><creator>Meinl, Alexandra</creator><creator>Hackl, Christa</creator><creator>Eibl, Johann</creator><creator>Gabriel, Christian</creator><creator>Hennerbichler, Simone</creator><creator>Redl, Heinz</creator><creator>Wolbank, Susanne</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20140601</creationdate><title>Intact human amniotic membrane differentiated towards the chondrogenic lineage</title><author>Lindenmair, Andrea ; Nürnberger, Sylvia ; Stadler, Guido ; Meinl, Alexandra ; Hackl, Christa ; Eibl, Johann ; Gabriel, Christian ; Hennerbichler, Simone ; Redl, Heinz ; Wolbank, Susanne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-71dc902d00be4c1215cf8ed13dea6d74a2f0c07dcea17d8c699f879784fe27883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amnion - cytology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biopsy</topic><topic>Cartilage - cytology</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Lineage - physiology</topic><topic>Cells, Cultured</topic><topic>Chondrogenesis - physiology</topic><topic>Female</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Membranes</topic><topic>Original Paper</topic><topic>Placenta - cytology</topic><topic>Pregnancy</topic><topic>Stem Cells - cytology</topic><topic>Tissues</topic><topic>Transplant Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lindenmair, Andrea</creatorcontrib><creatorcontrib>Nürnberger, Sylvia</creatorcontrib><creatorcontrib>Stadler, Guido</creatorcontrib><creatorcontrib>Meinl, Alexandra</creatorcontrib><creatorcontrib>Hackl, Christa</creatorcontrib><creatorcontrib>Eibl, Johann</creatorcontrib><creatorcontrib>Gabriel, Christian</creatorcontrib><creatorcontrib>Hennerbichler, Simone</creatorcontrib><creatorcontrib>Redl, Heinz</creatorcontrib><creatorcontrib>Wolbank, Susanne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cell and tissue banking</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lindenmair, Andrea</au><au>Nürnberger, Sylvia</au><au>Stadler, Guido</au><au>Meinl, Alexandra</au><au>Hackl, Christa</au><au>Eibl, Johann</au><au>Gabriel, Christian</au><au>Hennerbichler, Simone</au><au>Redl, Heinz</au><au>Wolbank, Susanne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intact human amniotic membrane differentiated towards the chondrogenic lineage</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Bank</stitle><addtitle>Cell Tissue Bank</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>15</volume><issue>2</issue><spage>213</spage><epage>225</epage><pages>213-225</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hAM represents a natural, preformed sheet including highly potent stem cells. In the present approach for cartilage regeneration we have induced chondrogenesis in hAM in vitro. For this, hAM biopsies were cultured for up to 56 days under chondrogenic conditions. The induced hAM was characterized for remaining viability, glycosaminoglycan (GAG) accumulation using histochemical analysis, and a quantitative assay. Collagen I, II and X was immunohistochemically determined and cartilage-specific mRNA expression of (sex determining region Y-) box 9, cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), versican (CSPG2), COL1A1, COL9A2, melanoma inhibitory activity (MIA), and cartilage-linking protein 1 (CRTL1) analyzed by quantitative real-time polymerase chain reaction. Human AM was successfully induced to accumulate GAG, as demonstrated by Alcianblue staining and a significant (
p
< 0.001) increase of GAG/viability under chondrogenic conditions peaking in a 29.9 ± 0.9-fold induction on day 56. Further, upon chondrogenic induction collagen II positive areas were identified within histological sections and cartilage-specific markers including COMP, AGC1, CSPG2, COL1A1, COL9A2, MIA, and CRTL1 were found upregulated at mRNA level. This is the first study, demonstrating that upon in vitro induction viable human amnion expresses cartilage-specific markers and accumulates GAGs within the biomatrix. This is a promising first step towards a potential use of living hAM for cartilage TE.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24828570</pmid><doi>10.1007/s10561-014-9454-9</doi><tpages>13</tpages></addata></record> |
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| subjects | Amnion - cytology Biomedical and Life Sciences Biomedicine Biopsy Cartilage - cytology Cell Biology Cell culture Cell Differentiation - physiology Cell Lineage - physiology Cells, Cultured Chondrogenesis - physiology Female Humans Life Sciences Membranes Original Paper Placenta - cytology Pregnancy Stem Cells - cytology Tissues Transplant Surgery |
| title | Intact human amniotic membrane differentiated towards the chondrogenic lineage |
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