Community structure and PAH ring-hydroxylating dioxygenase genes of a marine pyrene-degrading microbial consortium
Marine microbial consortium UBF, enriched from a beach polluted by the Prestige oil spill and highly efficient in degrading this heavy fuel, was subcultured in pyrene minimal medium. The pyrene-degrading subpopulation (UBF-Py) mineralized 31 % of pyrene without accumulation of partially oxidized int...
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| Veröffentlicht in: | Biodegradation (Dordrecht) 2014-07, Vol.25 (4), p.543-556 |
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| creator | Gallego, Sara Vila, Joaquim Tauler, Margalida Nieto, José María Breugelmans, Philip Springael, Dirk Grifoll, Magdalena |
| description | Marine microbial consortium UBF, enriched from a beach polluted by the Prestige oil spill and highly efficient in degrading this heavy fuel, was subcultured in pyrene minimal medium. The pyrene-degrading subpopulation (UBF-Py) mineralized 31 % of pyrene without accumulation of partially oxidized intermediates indicating the cooperation of different microbial components in substrate mineralization. The microbial community composition was characterized by culture dependent and PCR based methods (PCR-DGGE and clone libraries). Molecular analyses showed a highly stable community composed by
Alphaproteobacteria
(84 %,
Breoghania
,
Thalassospira
,
Paracoccus,
and
Martelella
) and
Actinobacteria
(16 %,
Gordonia
). The members of
Thalasosspira
and
Gordonia
were not recovered as pure cultures, but five additional strains, not detected in the molecular analysis, that classified within the genera
Novosphingobium, Sphingopyxis, Aurantimonas
(
Alphaproteobacteria
)
, Alcanivorax
(
Gammaproteobacteria
) and
Micrococcus
(
Actinobacteria
), were isolated. None of the isolates degraded pyrene or other PAHs in pure culture. PCR amplification of Gram-positive and Gram-negative dioxygenase genes did not produce results with any of the cultured strains. However, sequences related to the NidA3 pyrene dioxygenase present in mycobacterial strains were detected in UBF-Py consortium, suggesting the representative of
Gordonia
as the key pyrene degrader, which is consistent with a preeminent role of actinobacteria in pyrene removal in coastal environments affected by marine oil spills. |
| doi_str_mv | 10.1007/s10532-013-9680-z |
| format | Article |
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Alphaproteobacteria
(84 %,
Breoghania
,
Thalassospira
,
Paracoccus,
and
Martelella
) and
Actinobacteria
(16 %,
Gordonia
). The members of
Thalasosspira
and
Gordonia
were not recovered as pure cultures, but five additional strains, not detected in the molecular analysis, that classified within the genera
Novosphingobium, Sphingopyxis, Aurantimonas
(
Alphaproteobacteria
)
, Alcanivorax
(
Gammaproteobacteria
) and
Micrococcus
(
Actinobacteria
), were isolated. None of the isolates degraded pyrene or other PAHs in pure culture. PCR amplification of Gram-positive and Gram-negative dioxygenase genes did not produce results with any of the cultured strains. However, sequences related to the NidA3 pyrene dioxygenase present in mycobacterial strains were detected in UBF-Py consortium, suggesting the representative of
Gordonia
as the key pyrene degrader, which is consistent with a preeminent role of actinobacteria in pyrene removal in coastal environments affected by marine oil spills.</description><identifier>ISSN: 0923-9820</identifier><identifier>EISSN: 1572-9729</identifier><identifier>DOI: 10.1007/s10532-013-9680-z</identifier><identifier>PMID: 24356981</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Actinobacteria ; Alcanivorax ; Analysis ; Aquatic Pollution ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteria - metabolism ; Beaches ; Biodegradation ; Biodegradation of pollutants ; Biodegradation, Environmental ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Coastal environments ; Community composition ; Community structure ; Consortia ; Denaturing Gradient Gel Electrophoresis ; Dioxygenases - genetics ; Environment and pollution ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Bacterial ; Geochemistry ; Gordonia ; Heterotrophic Processes ; Hydroxylation ; Industrial applications and implications. Economical aspects ; Life Sciences ; Marine ; Microbial Consortia - genetics ; Microbiology ; Micrococcus ; Mineralization ; Molecular Sequence Data ; Mycobacterium ; Novosphingobium ; Oil pollution ; Oil spills ; Original Article ; Paracoccus ; Phenanthrenes - metabolism ; Phylogeny ; Polycyclic Aromatic Hydrocarbons - metabolism ; Pyrene ; Pyrenes - metabolism ; RNA, Ribosomal, 16S - genetics ; Seawater - microbiology ; Sequence Analysis, DNA ; Soil Science & Conservation ; Sphingopyxis ; Terrestrial Pollution ; Waste Management/Waste Technology ; Waste Water Technology ; Water Management ; Water Pollution Control</subject><ispartof>Biodegradation (Dordrecht), 2014-07, Vol.25 (4), p.543-556</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2014 Springer</rights><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-84d15f112074d2387bc7b6c57f34c95861162931ec455bfd6efe24833cad09f73</citedby><cites>FETCH-LOGICAL-c540t-84d15f112074d2387bc7b6c57f34c95861162931ec455bfd6efe24833cad09f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10532-013-9680-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10532-013-9680-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28609954$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24356981$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gallego, Sara</creatorcontrib><creatorcontrib>Vila, Joaquim</creatorcontrib><creatorcontrib>Tauler, Margalida</creatorcontrib><creatorcontrib>Nieto, José María</creatorcontrib><creatorcontrib>Breugelmans, Philip</creatorcontrib><creatorcontrib>Springael, Dirk</creatorcontrib><creatorcontrib>Grifoll, Magdalena</creatorcontrib><title>Community structure and PAH ring-hydroxylating dioxygenase genes of a marine pyrene-degrading microbial consortium</title><title>Biodegradation (Dordrecht)</title><addtitle>Biodegradation</addtitle><addtitle>Biodegradation</addtitle><description>Marine microbial consortium UBF, enriched from a beach polluted by the Prestige oil spill and highly efficient in degrading this heavy fuel, was subcultured in pyrene minimal medium. The pyrene-degrading subpopulation (UBF-Py) mineralized 31 % of pyrene without accumulation of partially oxidized intermediates indicating the cooperation of different microbial components in substrate mineralization. The microbial community composition was characterized by culture dependent and PCR based methods (PCR-DGGE and clone libraries). Molecular analyses showed a highly stable community composed by
Alphaproteobacteria
(84 %,
Breoghania
,
Thalassospira
,
Paracoccus,
and
Martelella
) and
Actinobacteria
(16 %,
Gordonia
). The members of
Thalasosspira
and
Gordonia
were not recovered as pure cultures, but five additional strains, not detected in the molecular analysis, that classified within the genera
Novosphingobium, Sphingopyxis, Aurantimonas
(
Alphaproteobacteria
)
, Alcanivorax
(
Gammaproteobacteria
) and
Micrococcus
(
Actinobacteria
), were isolated. None of the isolates degraded pyrene or other PAHs in pure culture. PCR amplification of Gram-positive and Gram-negative dioxygenase genes did not produce results with any of the cultured strains. However, sequences related to the NidA3 pyrene dioxygenase present in mycobacterial strains were detected in UBF-Py consortium, suggesting the representative of
Gordonia
as the key pyrene degrader, which is consistent with a preeminent role of actinobacteria in pyrene removal in coastal environments affected by marine oil spills.</description><subject>Actinobacteria</subject><subject>Alcanivorax</subject><subject>Analysis</subject><subject>Aquatic Pollution</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Beaches</subject><subject>Biodegradation</subject><subject>Biodegradation of pollutants</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Coastal environments</subject><subject>Community composition</subject><subject>Community structure</subject><subject>Consortia</subject><subject>Denaturing Gradient Gel Electrophoresis</subject><subject>Dioxygenases - genetics</subject><subject>Environment and pollution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Geochemistry</subject><subject>Gordonia</subject><subject>Heterotrophic Processes</subject><subject>Hydroxylation</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Life Sciences</subject><subject>Marine</subject><subject>Microbial Consortia - genetics</subject><subject>Microbiology</subject><subject>Micrococcus</subject><subject>Mineralization</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium</subject><subject>Novosphingobium</subject><subject>Oil pollution</subject><subject>Oil spills</subject><subject>Original Article</subject><subject>Paracoccus</subject><subject>Phenanthrenes - metabolism</subject><subject>Phylogeny</subject><subject>Polycyclic Aromatic Hydrocarbons - metabolism</subject><subject>Pyrene</subject><subject>Pyrenes - metabolism</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Seawater - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Soil Science & Conservation</subject><subject>Sphingopyxis</subject><subject>Terrestrial Pollution</subject><subject>Waste Management/Waste Technology</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><issn>0923-9820</issn><issn>1572-9729</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc-L1TAQx4Mo7tvVP8CLBETw0nWSNE1zfDzUFRb0oOeQ5sczS9s8kxbs_vWm9LmKIEgOk5l8ZiYzX4ReELgmAOJtJsAZrYCwSjYtVPeP0I5wQSspqHyMdiBpeWkpXKDLnO8AQAqgT9EFrRlvZEt2KB3iMMxjmBacpzSbaU4O69Hiz_sbnMJ4rL4tNsUfS6-n4mEbyv3oRp0dLsZlHD3WeNCFdfi0pBKrrDsmbVd8CCbFLugemzjmmKYwD8_QE6_77J6f7RX6-v7dl8NNdfvpw8fD_rYyvIapamtLuCeEgqgtZa3ojOgaw4VntZG8bQhpqGTEmZrzztvGeUfrljGjLUgv2BV6s9U9pfh9dnlSQ8jG9b0eXZyzIqUNZaTl9D9QVoPkspEFffUXehfnNJZBVopKEJyRQl1v1FH3ToXRxylpU451ZSVxdD6U-F4QSmVDYS1LtoSyr5yT8-qUQtnqogioVWy1ia2K2GoVW92XnJfnr8zd4OxDxi91C_D6DOhsdO-THk3Iv7m2ASl5XTi6cfm0Su7SHzP9s_tPSX3B3Q</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Gallego, Sara</creator><creator>Vila, Joaquim</creator><creator>Tauler, Margalida</creator><creator>Nieto, José María</creator><creator>Breugelmans, Philip</creator><creator>Springael, Dirk</creator><creator>Grifoll, Magdalena</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>SOI</scope><scope>7X8</scope><scope>7QH</scope><scope>7QO</scope><scope>7TN</scope><scope>7TV</scope><scope>RC3</scope></search><sort><creationdate>20140701</creationdate><title>Community structure and PAH ring-hydroxylating dioxygenase genes of a marine pyrene-degrading microbial consortium</title><author>Gallego, Sara ; Vila, Joaquim ; Tauler, Margalida ; Nieto, José María ; Breugelmans, Philip ; Springael, Dirk ; Grifoll, Magdalena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-84d15f112074d2387bc7b6c57f34c95861162931ec455bfd6efe24833cad09f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Actinobacteria</topic><topic>Alcanivorax</topic><topic>Analysis</topic><topic>Aquatic Pollution</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - metabolism</topic><topic>Beaches</topic><topic>Biodegradation</topic><topic>Biodegradation of pollutants</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Coastal environments</topic><topic>Community composition</topic><topic>Community structure</topic><topic>Consortia</topic><topic>Denaturing Gradient Gel Electrophoresis</topic><topic>Dioxygenases - genetics</topic><topic>Environment and pollution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Geochemistry</topic><topic>Gordonia</topic><topic>Heterotrophic Processes</topic><topic>Hydroxylation</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Life Sciences</topic><topic>Marine</topic><topic>Microbial Consortia - genetics</topic><topic>Microbiology</topic><topic>Micrococcus</topic><topic>Mineralization</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium</topic><topic>Novosphingobium</topic><topic>Oil pollution</topic><topic>Oil spills</topic><topic>Original Article</topic><topic>Paracoccus</topic><topic>Phenanthrenes - metabolism</topic><topic>Phylogeny</topic><topic>Polycyclic Aromatic Hydrocarbons - metabolism</topic><topic>Pyrene</topic><topic>Pyrenes - metabolism</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Seawater - microbiology</topic><topic>Sequence Analysis, DNA</topic><topic>Soil Science & Conservation</topic><topic>Sphingopyxis</topic><topic>Terrestrial Pollution</topic><topic>Waste Management/Waste Technology</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallego, Sara</creatorcontrib><creatorcontrib>Vila, Joaquim</creatorcontrib><creatorcontrib>Tauler, Margalida</creatorcontrib><creatorcontrib>Nieto, José María</creatorcontrib><creatorcontrib>Breugelmans, Philip</creatorcontrib><creatorcontrib>Springael, Dirk</creatorcontrib><creatorcontrib>Grifoll, Magdalena</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biodegradation (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallego, Sara</au><au>Vila, Joaquim</au><au>Tauler, Margalida</au><au>Nieto, José María</au><au>Breugelmans, Philip</au><au>Springael, Dirk</au><au>Grifoll, Magdalena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Community structure and PAH ring-hydroxylating dioxygenase genes of a marine pyrene-degrading microbial consortium</atitle><jtitle>Biodegradation (Dordrecht)</jtitle><stitle>Biodegradation</stitle><addtitle>Biodegradation</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>25</volume><issue>4</issue><spage>543</spage><epage>556</epage><pages>543-556</pages><issn>0923-9820</issn><eissn>1572-9729</eissn><abstract>Marine microbial consortium UBF, enriched from a beach polluted by the Prestige oil spill and highly efficient in degrading this heavy fuel, was subcultured in pyrene minimal medium. The pyrene-degrading subpopulation (UBF-Py) mineralized 31 % of pyrene without accumulation of partially oxidized intermediates indicating the cooperation of different microbial components in substrate mineralization. The microbial community composition was characterized by culture dependent and PCR based methods (PCR-DGGE and clone libraries). Molecular analyses showed a highly stable community composed by
Alphaproteobacteria
(84 %,
Breoghania
,
Thalassospira
,
Paracoccus,
and
Martelella
) and
Actinobacteria
(16 %,
Gordonia
). The members of
Thalasosspira
and
Gordonia
were not recovered as pure cultures, but five additional strains, not detected in the molecular analysis, that classified within the genera
Novosphingobium, Sphingopyxis, Aurantimonas
(
Alphaproteobacteria
)
, Alcanivorax
(
Gammaproteobacteria
) and
Micrococcus
(
Actinobacteria
), were isolated. None of the isolates degraded pyrene or other PAHs in pure culture. PCR amplification of Gram-positive and Gram-negative dioxygenase genes did not produce results with any of the cultured strains. However, sequences related to the NidA3 pyrene dioxygenase present in mycobacterial strains were detected in UBF-Py consortium, suggesting the representative of
Gordonia
as the key pyrene degrader, which is consistent with a preeminent role of actinobacteria in pyrene removal in coastal environments affected by marine oil spills.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24356981</pmid><doi>10.1007/s10532-013-9680-z</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0923-9820 |
| ispartof | Biodegradation (Dordrecht), 2014-07, Vol.25 (4), p.543-556 |
| issn | 0923-9820 1572-9729 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1540231852 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Actinobacteria Alcanivorax Analysis Aquatic Pollution Bacteria - genetics Bacteria - isolation & purification Bacteria - metabolism Beaches Biodegradation Biodegradation of pollutants Biodegradation, Environmental Biological and medical sciences Biomedical and Life Sciences Biotechnology Coastal environments Community composition Community structure Consortia Denaturing Gradient Gel Electrophoresis Dioxygenases - genetics Environment and pollution Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Geochemistry Gordonia Heterotrophic Processes Hydroxylation Industrial applications and implications. Economical aspects Life Sciences Marine Microbial Consortia - genetics Microbiology Micrococcus Mineralization Molecular Sequence Data Mycobacterium Novosphingobium Oil pollution Oil spills Original Article Paracoccus Phenanthrenes - metabolism Phylogeny Polycyclic Aromatic Hydrocarbons - metabolism Pyrene Pyrenes - metabolism RNA, Ribosomal, 16S - genetics Seawater - microbiology Sequence Analysis, DNA Soil Science & Conservation Sphingopyxis Terrestrial Pollution Waste Management/Waste Technology Waste Water Technology Water Management Water Pollution Control |
| title | Community structure and PAH ring-hydroxylating dioxygenase genes of a marine pyrene-degrading microbial consortium |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T09%3A45%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Community%20structure%20and%20PAH%20ring-hydroxylating%20dioxygenase%20genes%20of%20a%20marine%20pyrene-degrading%20microbial%20consortium&rft.jtitle=Biodegradation%20(Dordrecht)&rft.au=Gallego,%20Sara&rft.date=2014-07-01&rft.volume=25&rft.issue=4&rft.spage=543&rft.epage=556&rft.pages=543-556&rft.issn=0923-9820&rft.eissn=1572-9729&rft_id=info:doi/10.1007/s10532-013-9680-z&rft_dat=%3Cgale_proqu%3EA712296209%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1532907531&rft_id=info:pmid/24356981&rft_galeid=A712296209&rfr_iscdi=true |