DNA repair in normal human and xeroderma pigmentosum group A fibroblasts following treatment with various methanesulfonates and the demonstration of a long-patch (u.v.-like) repair component
Excision repair of DNA in normal and xeroderma pigmentosum complementation group A fibroblasts was examined following treatment with methyl-, ethyl-, and isopropyl methanesulfonate. Studies utilizing repair synthesis methods and inhibition with arabinofuranosyl cytosine revealed two distinct phases...
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| Veröffentlicht in: | Carcinogenesis (N.Y.); (United States) 1982, Vol.3 (1), p.7-14 |
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| creator | Snyder, Ronald D. Regan, James D. |
| description | Excision repair of DNA in normal and xeroderma pigmentosum complementation group A fibroblasts was examined following treatment with methyl-, ethyl-, and isopropyl methanesulfonate. Studies utilizing repair synthesis methods and inhibition with arabinofuranosyl cytosine revealed two distinct phases of repair; one commencing and terminating within the first 3–5 h after the treatment, and a second, much longer phase extending from 9–35 h post-treatment. Both phases of repair have a long-patch (u.v.-like) component, which establishes for the first time the existence of this mode of repair in response to alkane sulfonate damage. While xeroderma cells display somewhat fewer alkaline labile sites in their DNA following alkylation treatment than do their normal counterparts (consistent with earlier observations suggesting a deficiency in a glycosylase or apurinic endonuclease), we are unable to demonstrate a deficiency of these cells in either of the two phases of repair. |
| doi_str_mv | 10.1093/carcin/3.1.7 |
| format | Article |
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Studies utilizing repair synthesis methods and inhibition with arabinofuranosyl cytosine revealed two distinct phases of repair; one commencing and terminating within the first 3–5 h after the treatment, and a second, much longer phase extending from 9–35 h post-treatment. Both phases of repair have a long-patch (u.v.-like) component, which establishes for the first time the existence of this mode of repair in response to alkane sulfonate damage. 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POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. 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Studies utilizing repair synthesis methods and inhibition with arabinofuranosyl cytosine revealed two distinct phases of repair; one commencing and terminating within the first 3–5 h after the treatment, and a second, much longer phase extending from 9–35 h post-treatment. Both phases of repair have a long-patch (u.v.-like) component, which establishes for the first time the existence of this mode of repair in response to alkane sulfonate damage. While xeroderma cells display somewhat fewer alkaline labile sites in their DNA following alkylation treatment than do their normal counterparts (consistent with earlier observations suggesting a deficiency in a glycosylase or apurinic endonuclease), we are unable to demonstrate a deficiency of these cells in either of the two phases of repair.</description><subject>550400 - Genetics</subject><subject>560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)</subject><subject>ALKANES</subject><subject>Alkylating Agents - toxicity</subject><subject>ALKYLATION</subject><subject>ANIMAL CELLS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RECOVERY</subject><subject>BIOLOGICAL REPAIR</subject><subject>CHEMICAL REACTIONS</subject><subject>COMPARATIVE EVALUATIONS</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>Cytarabine - pharmacology</subject><subject>DECOMPOSITION</subject><subject>DISEASES</subject><subject>DNA</subject><subject>DNA Repair - drug effects</subject><subject>FIBROBLASTS</subject><subject>Fibroblasts - metabolism</subject><subject>GENETIC EFFECTS</subject><subject>Humans</subject><subject>HYDROCARBONS</subject><subject>Mesylates - toxicity</subject><subject>METHANE</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC SULFUR COMPOUNDS</subject><subject>PHOTOCHEMICAL REACTIONS</subject><subject>PHOTOLYSIS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RECOVERY</subject><subject>REPAIR</subject><subject>SKIN DISEASES</subject><subject>SOMATIC CELLS</subject><subject>SULFONATES</subject><subject>TIME DEPENDENCE</subject><subject>Ultraviolet Rays</subject><subject>XERODERMA PIGMENTOSUM</subject><subject>Xeroderma Pigmentosum - genetics</subject><subject>Xeroderma Pigmentosum - metabolism</subject><issn>0143-3334</issn><issn>1460-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUtv1DAUhSMEKkNhxxbJYlGBRFI7zjjJchgeRapAwCAhNtaNcz0xTexgO235c_w2MmTo6kr3fDr3cZLkKaMZozU_V-CVsec8Y1l5L1mxQtA0ZxW9n6woK3jKOS8eJo9C-EkpE3xdnyQnIi9rnher5M-bjxvicQTjibHEOj9AT7ppAEvAtuQWvWtxbpLR7Ae00YVpIHvvppFsiDaNd00PIQaiXd-7G2P3JHqEeGDJjYkduQZv3BTIgLEDi2HqtbMQMfwbEDskLQ7OhughGmeJ0wRI7-w-HSGqjryYsuss7c0Vvvy_qXLD6Ow84XHyQEMf8Mmxnibf3r3dbS_Sy0_vP2w3l6niVR5ToURZrFmlKNNUYwO1apFyaITQFQVELKoKlUBONacAQjRY0ragDde80ZSfJs8XXxeikUGZiKpTzlpUUZa0KoqqnKGzBRq9-zVhiHIwQWHfz1fPD5BsXVBW1vkMvlpA5V0IHrUcvRnA_5aMykOmcslUcsnkwffZ0XdqBmzv4GOIs54uugkRb-9k8FdSlLxcy4vvP2T1dffl867eytf8L0ByssI</recordid><startdate>1982</startdate><enddate>1982</enddate><creator>Snyder, Ronald D.</creator><creator>Regan, James D.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>OTOTI</scope></search><sort><creationdate>1982</creationdate><title>DNA repair in normal human and xeroderma pigmentosum group A fibroblasts following treatment with various methanesulfonates and the demonstration of a long-patch (u.v.-like) repair component</title><author>Snyder, Ronald D. ; Regan, James D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-6c674518c01f0feba9cde03ab66f80aeee488ec6e30f30aa66be70d40b3f3bf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>550400 - Genetics</topic><topic>560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)</topic><topic>ALKANES</topic><topic>Alkylating Agents - toxicity</topic><topic>ALKYLATION</topic><topic>ANIMAL CELLS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>CHEMICAL REACTIONS</topic><topic>COMPARATIVE EVALUATIONS</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>Cytarabine - pharmacology</topic><topic>DECOMPOSITION</topic><topic>DISEASES</topic><topic>DNA</topic><topic>DNA Repair - drug effects</topic><topic>FIBROBLASTS</topic><topic>Fibroblasts - metabolism</topic><topic>GENETIC EFFECTS</topic><topic>Humans</topic><topic>HYDROCARBONS</topic><topic>Mesylates - toxicity</topic><topic>METHANE</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC SULFUR COMPOUNDS</topic><topic>PHOTOCHEMICAL REACTIONS</topic><topic>PHOTOLYSIS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RECOVERY</topic><topic>REPAIR</topic><topic>SKIN DISEASES</topic><topic>SOMATIC CELLS</topic><topic>SULFONATES</topic><topic>TIME DEPENDENCE</topic><topic>Ultraviolet Rays</topic><topic>XERODERMA PIGMENTOSUM</topic><topic>Xeroderma Pigmentosum - genetics</topic><topic>Xeroderma Pigmentosum - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Snyder, Ronald D.</creatorcontrib><creatorcontrib>Regan, James D.</creatorcontrib><creatorcontrib>University of Tennessee, Oak Ridge Graduate School of Biomedical Science, Oak Ridge, TN</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV</collection><jtitle>Carcinogenesis (N.Y.); (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Snyder, Ronald D.</au><au>Regan, James D.</au><aucorp>University of Tennessee, Oak Ridge Graduate School of Biomedical Science, Oak Ridge, TN</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA repair in normal human and xeroderma pigmentosum group A fibroblasts following treatment with various methanesulfonates and the demonstration of a long-patch (u.v.-like) repair component</atitle><jtitle>Carcinogenesis (N.Y.); (United States)</jtitle><addtitle>Carcinogenesis</addtitle><date>1982</date><risdate>1982</risdate><volume>3</volume><issue>1</issue><spage>7</spage><epage>14</epage><pages>7-14</pages><issn>0143-3334</issn><eissn>1460-2180</eissn><abstract>Excision repair of DNA in normal and xeroderma pigmentosum complementation group A fibroblasts was examined following treatment with methyl-, ethyl-, and isopropyl methanesulfonate. Studies utilizing repair synthesis methods and inhibition with arabinofuranosyl cytosine revealed two distinct phases of repair; one commencing and terminating within the first 3–5 h after the treatment, and a second, much longer phase extending from 9–35 h post-treatment. Both phases of repair have a long-patch (u.v.-like) component, which establishes for the first time the existence of this mode of repair in response to alkane sulfonate damage. While xeroderma cells display somewhat fewer alkaline labile sites in their DNA following alkylation treatment than do their normal counterparts (consistent with earlier observations suggesting a deficiency in a glycosylase or apurinic endonuclease), we are unable to demonstrate a deficiency of these cells in either of the two phases of repair.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>6279324</pmid><doi>10.1093/carcin/3.1.7</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0143-3334 |
| ispartof | Carcinogenesis (N.Y.); (United States), 1982, Vol.3 (1), p.7-14 |
| issn | 0143-3334 1460-2180 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15401792 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | 550400 - Genetics 560301 - Chemicals Metabolism & Toxicology- Cells- (-1987) ALKANES Alkylating Agents - toxicity ALKYLATION ANIMAL CELLS BASIC BIOLOGICAL SCIENCES BIOLOGICAL EFFECTS BIOLOGICAL RECOVERY BIOLOGICAL REPAIR CHEMICAL REACTIONS COMPARATIVE EVALUATIONS CONNECTIVE TISSUE CELLS Cytarabine - pharmacology DECOMPOSITION DISEASES DNA DNA Repair - drug effects FIBROBLASTS Fibroblasts - metabolism GENETIC EFFECTS Humans HYDROCARBONS Mesylates - toxicity METHANE NUCLEIC ACIDS ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS PHOTOCHEMICAL REACTIONS PHOTOLYSIS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RECOVERY REPAIR SKIN DISEASES SOMATIC CELLS SULFONATES TIME DEPENDENCE Ultraviolet Rays XERODERMA PIGMENTOSUM Xeroderma Pigmentosum - genetics Xeroderma Pigmentosum - metabolism |
| title | DNA repair in normal human and xeroderma pigmentosum group A fibroblasts following treatment with various methanesulfonates and the demonstration of a long-patch (u.v.-like) repair component |
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